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The map label for this gene is purN [H]

Identifier: 94311810

GI number: 94311810

Start: 3129369

End: 3130031

Strand: Reverse

Name: purN [H]

Synonym: Rmet_2878

Alternate gene names: 94311810

Gene position: 3130031-3129369 (Counterclockwise)

Preceding gene: 94311813

Following gene: 94311809

Centisome position: 79.68

GC content: 61.39

Gene sequence:

>663_bases
ATGAAGAATATTGTGATATTGATTTCCGGGCGTGGCTCCAACATGGAAGCGATCGTCCGGGCCTGCGCCGCCGAGAAATG
GCCGGCGCGCGTGGCAGCGGTACTGTCGAACCGCCCCGATGCTTCGGGCCTGCAGTTCGCCTCGCGCCACGGTATTGCCA
CCGGTGTGGTGGACCACAAGCAGTTTTCCGGACGTGAGAGTTTCGACGCCGCGATGCGGGACGCCATCGACGCCTACCAG
CCCGACCTCATCGTGCTGGCAGGCTTCATGCGGATCCTGACGCCTGGCTTCGTTGAGCATTACGCCGGTCGAATGCTCAA
CATCCATCCGTCGCTGCTGCCGAGCTTCCCCGGGTTGCATACGCACAAGCAGGCGTTGGAGGCGGGCGTCAAGCTGCATG
GCGCGACAGTGCATTTCGTGACGCCAGAACTCGATCATGGTCCGATCGTGTTGCAGGCGGCACTTGATGTTCTGCCTGGC
GATACACCGGAGTCGCTGGCCGATCGTTTGCTCGATAGCGAACATGTCATTTACCCGCGCGCCGTGCGCTGGTTCGTCGA
AGACCGTCTGCACGTGCAGGACGGTGTCGTTCAAGTAGTTCAACCGGCCGAGCCCCAATGGTTCATGGCCATCTCGCCCC
AAGCGGCAGGAAAACAGCCATGA

Upstream 100 bases:

>100_bases
GCGCGCCTGTTTTTGTTGCCCCATGTGCGTTGGCGGTAATTAAGTACCTAATGCGCATGGTTTGCAAGTGTTTGGACCAC
AGCTTGGTAGAATCGCGGCG

Downstream 100 bases:

>100_bases
GTCGCAATCCCGCAACTTCCCAAGACAATCGCCAGCGCTCGCGTCCGAGCAAAGGCAAGGCCAGCCCGATCCGCAAGTCG
CCCAACGCCCGGCAGCAGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHKQFSGRESFDAAMRDAIDAYQ
PDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLHTHKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPG
DTPESLADRLLDSEHVIYPRAVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP

Sequences:

>Translated_220_residues
MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHKQFSGRESFDAAMRDAIDAYQ
PDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLHTHKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPG
DTPESLADRLLDSEHVIYPRAVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP
>Mature_220_residues
MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHKQFSGRESFDAAMRDAIDAYQ
PDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLHTHKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPG
DTPESLADRLLDSEHVIYPRAVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=42.156862745098, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=42.156862745098, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=42.156862745098, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=53.3678756476684, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI1787483, Length=191, Percent_Identity=36.1256544502618, Blast_Score=114, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=37.4301675977654, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320616, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=47.3404255319149, Blast_Score=172, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23983; Mature: 23983

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHK
CCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCEECCCCCH
QFSGRESFDAAMRDAIDAYQPDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLH
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCCC
THKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPGDTPESLADRLLDSEHVIYPR
HHHHHHHCCCEECCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHH
AVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP
HHHHHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHK
CCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCEECCCCCH
QFSGRESFDAAMRDAIDAYQPDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLH
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCCC
THKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPGDTPESLADRLLDSEHVIYPR
HHHHHHHCCCEECCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHH
AVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP
HHHHHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]