Click here to switch to the map view.

The map label for this gene is ascD [H]

Identifier: 94311793

GI number: 94311793

Start: 3110503

End: 3111558

Strand: Direct

Name: ascD [H]

Synonym: Rmet_2861

Alternate gene names: 94311793

Gene position: 3110503-3111558 (Clockwise)

Preceding gene: 94311781

Following gene: 94311794

Centisome position: 79.19

GC content: 66.0

Gene sequence:

>1056_bases
ATGGCGTATCAAGTCACCGTAATGCCCAGCGGCCACAAGTTCGAAGTGGCCACCGATGAAACCATCCTTGGCGCGGCCCT
GCGCCACAGCATTGGATTGCCCTACGGGTGCAAGAACGGTGCGTGTGGCTCCTGCAAGGGCCGCGTGCTCGAAGGCACGA
TCCACCAGGGCGACCACGCCGCCGCGGCCCTGACCGCCCAGGAAAAAACGGAAGGCCGCGCGCTGTTCTGCTGCGCCAAC
GCCACTTCCGACATTACGATCGAATGCCGCGAGGTGCATGGCGCCGGCGATATTCCGATCAAAAAGATCCCCTGCCGCGT
GGCCTCGGTGGAGCGCCTGGCCGACGACGTCATCGCCATCAAGCTGCAACTGCCCGCCACCGAGCGCATGCAGTACCTGG
CCGGCCAGTACGTGGAATTCCTGCTGCGTGACGGCAAGCGCCGCAGCTACTCGATCGCCACGCCGCCGCACGAGGAAGGC
CCGATCGAACTGCATATCCGCCACATGCCTGGCGGCGCGTTCACCGACTACGTATTCGGCGCCAAGGAAGGCGCGCCGGC
CATGAAGGAGCGCGACATCCTGCGCTTCGAAGGCCCGCTCGGCAGCTTCTTCCTGCGCGAGGAATCGGACAAGCCGATCA
TCCTGCTGGCCTCCGGCACCGGCTTCGCCCCGATCAAGGCGATTGTCGAACATGCCGCGTACACGGGCATCACCCGTCCG
ATGACGCTGTACTGGGGCGGACGCCGTCCGCGCGACCTGTACATGCACGCGCTGTGCGAACAGTGGGCGCGCGACCTCCC
GAACTTCAAGTACGTGCCGGTGGTATCCGACGCGCAGGCTGACGACAACTGGACCGGCCGCACCGGCTTCGTTCACCAGG
CGGTGATGGCCGACCATCCGGACCTGTCCGGCCACGAGGTCTACGCCTGCGGCGCGCCCGTGATGGTGAACGCCGCGCGC
GGCGACTTCTCGGCGCAATGCAAGCTGCACGAGGACGCGTTCTTCGCCGACTCGTTCACGTCAGAAGCAGACCTGCACAA
GGGTGGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GCAACCGACGATCAGCAGGCGCGGACGACCCAGGCGCCGTGCGCCCCGTGGGGAAGACAATGCCGTTCGCGGCGACAGAA
TTTGGCTCATAGATCCGATT

Downstream 100 bases:

>100_bases
GGGCATTGCCCCCAGAACGCCACTCGCCGTTCCGCTGACACAACAATGGCACCCGGGCCTGCCAAATTTCGCTTTGACAC
CCGGGCCCATGCGTCTTATA

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAYQVTVMPSGHKFEVATDETILGAALRHSIGLPYGCKNGACGSCKGRVLEGTIHQGDHAAAALTAQEKTEGRALFCCAN
ATSDITIECREVHGAGDIPIKKIPCRVASVERLADDVIAIKLQLPATERMQYLAGQYVEFLLRDGKRRSYSIATPPHEEG
PIELHIRHMPGGAFTDYVFGAKEGAPAMKERDILRFEGPLGSFFLREESDKPIILLASGTGFAPIKAIVEHAAYTGITRP
MTLYWGGRRPRDLYMHALCEQWARDLPNFKYVPVVSDAQADDNWTGRTGFVHQAVMADHPDLSGHEVYACGAPVMVNAAR
GDFSAQCKLHEDAFFADSFTSEADLHKGGAA

Sequences:

>Translated_351_residues
MAYQVTVMPSGHKFEVATDETILGAALRHSIGLPYGCKNGACGSCKGRVLEGTIHQGDHAAAALTAQEKTEGRALFCCAN
ATSDITIECREVHGAGDIPIKKIPCRVASVERLADDVIAIKLQLPATERMQYLAGQYVEFLLRDGKRRSYSIATPPHEEG
PIELHIRHMPGGAFTDYVFGAKEGAPAMKERDILRFEGPLGSFFLREESDKPIILLASGTGFAPIKAIVEHAAYTGITRP
MTLYWGGRRPRDLYMHALCEQWARDLPNFKYVPVVSDAQADDNWTGRTGFVHQAVMADHPDLSGHEVYACGAPVMVNAAR
GDFSAQCKLHEDAFFADSFTSEADLHKGGAA
>Mature_350_residues
AYQVTVMPSGHKFEVATDETILGAALRHSIGLPYGCKNGACGSCKGRVLEGTIHQGDHAAAALTAQEKTEGRALFCCANA
TSDITIECREVHGAGDIPIKKIPCRVASVERLADDVIAIKLQLPATERMQYLAGQYVEFLLRDGKRRSYSIATPPHEEGP
IELHIRHMPGGAFTDYVFGAKEGAPAMKERDILRFEGPLGSFFLREESDKPIILLASGTGFAPIKAIVEHAAYTGITRPM
TLYWGGRRPRDLYMHALCEQWARDLPNFKYVPVVSDAQADDNWTGRTGFVHQAVMADHPDLSGHEVYACGAPVMVNAARG
DFSAQCKLHEDAFFADSFTSEADLHKGGAA

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=242, Percent_Identity=34.7107438016529, Blast_Score=132, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322699, Length=257, Percent_Identity=23.7354085603113, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 38205; Mature: 38074

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQVTVMPSGHKFEVATDETILGAALRHSIGLPYGCKNGACGSCKGRVLEGTIHQGDHA
CEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
AAALTAQEKTEGRALFCCANATSDITIECREVHGAGDIPIKKIPCRVASVERLADDVIAI
EEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCHHHCCCHHHHHHHHCCCEEEE
KLQLPATERMQYLAGQYVEFLLRDGKRRSYSIATPPHEEGPIELHIRHMPGGAFTDYVFG
EEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHEEC
AKEGAPAMKERDILRFEGPLGSFFLREESDKPIILLASGTGFAPIKAIVEHAAYTGITRP
CCCCCCCHHHCCEEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
MTLYWGGRRPRDLYMHALCEQWARDLPNFKYVPVVSDAQADDNWTGRTGFVHQAVMADHP
EEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
DLSGHEVYACGAPVMVNAARGDFSAQCKLHEDAFFADSFTSEADLHKGGAA
CCCCCEEEEECCCEEEEECCCCCEEEEEECCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYQVTVMPSGHKFEVATDETILGAALRHSIGLPYGCKNGACGSCKGRVLEGTIHQGDHA
EEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
AAALTAQEKTEGRALFCCANATSDITIECREVHGAGDIPIKKIPCRVASVERLADDVIAI
EEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCHHHCCCHHHHHHHHCCCEEEE
KLQLPATERMQYLAGQYVEFLLRDGKRRSYSIATPPHEEGPIELHIRHMPGGAFTDYVFG
EEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHEEC
AKEGAPAMKERDILRFEGPLGSFFLREESDKPIILLASGTGFAPIKAIVEHAAYTGITRP
CCCCCCCHHHCCEEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
MTLYWGGRRPRDLYMHALCEQWARDLPNFKYVPVVSDAQADDNWTGRTGFVHQAVMADHP
EEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
DLSGHEVYACGAPVMVNAARGDFSAQCKLHEDAFFADSFTSEADLHKGGAA
CCCCCEEEEECCCEEEEECCCCCEEEEEECCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]