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The map label for this gene is cysB [H]

Identifier: 94311750

GI number: 94311750

Start: 3065632

End: 3066573

Strand: Direct

Name: cysB [H]

Synonym: Rmet_2818

Alternate gene names: 94311750

Gene position: 3065632-3066573 (Clockwise)

Preceding gene: 94311742

Following gene: 94311751

Centisome position: 78.04

GC content: 60.3

Gene sequence:

>942_bases
ATGAACCTTCATCAATTCCGCTTTGTGCGTGAGGCTGTCCGTCAGAATTTCAACTTGACGGAGGCCGCAAAGGCGCTCTA
TACGTCACAACCCGGCGTTTCCAAGGCGATTATCGAACTGGAAGAGGAGCTAGGTGTCGATATCTTCACACGACACGGCA
AACGGATTCGCAGCCTGACCGAACCGGGGCGGCGCATCCTGACCTCGGTAGAGAAGATCCTGCAGGAAGTGGAAAGTCTG
AAGCGCGTGGGCAAGGACTACGCGGCACAGGATCAGGGCAACTTCACCATCGCCACCACCCATACCCAGGCCCGTTACGC
GCTGCCGCGCGTGATCAGCGAGTTCACCAAGCGCTACCCGAAGGTGCGGCTGTCGATCCAGCAGGGCAATCCGGCGCAGA
TCGCCGACATGATCTTGCATGACCAGGCCGATCTGGGCATCGCCACCGAGGGCATCTCGGGCGACAAGAGCCTGGTGTCG
CTGCCCGGGTACCAGTGGCAGCATATGGTGATTACGCCACCTGACCATCCGCTGCTGGAGAAAAAGCACCTGACGCTGGA
AGACCTGGCCACCTTTCCGCTGATCACCTATGACGTGAACTTTGCTGGCCGGCCCAAGATCGACAAGGCATTCGAGTTGC
GCCATCTCCAGCCGGACATCGTGCTGGAGGCCATCGACGCCGACGTCATCAAGACGTACGTCGAGGTTGGCCTGGGCGTC
GGCATCGTGGCCGGCCTTGCCTACGACCCCGAACGTGACCGCAACCTGCGCGGCATTCCGGCCGGCCACCTGTTTGGCAC
CAATGTCACCCACCTGGCCGTCAAGCAGGGGGCCTATCTACGCAGCTTCGTCTATACATTCATCGAGTTGTTCTCGCCGA
CCCTGAACCGCAAGCTGGTGGAACAGGCGATGTCGGGCGACCACGAAGCCTACGAACTCTGA

Upstream 100 bases:

>100_bases
TACGGGCAGGCCGAACGTTTAACAAAGTTCGAGAATCGTAATAAACTCGCCTCATACCTCAAACTAATAAGAAGTTGTTT
GTTTATACGGACGCAGTTAT

Downstream 100 bases:

>100_bases
GCATCGCTCGAAGCACCCTGCCCTATATATATGTCAGTCACTGATTCCCTCCCGACGATCCACTGGACCGAAGACGGCGC
CGAGCGCAGCGCGATGTGGC

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILTSVEKILQEVESL
KRVGKDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPAQIADMILHDQADLGIATEGISGDKSLVS
LPGYQWQHMVITPPDHPLLEKKHLTLEDLATFPLITYDVNFAGRPKIDKAFELRHLQPDIVLEAIDADVIKTYVEVGLGV
GIVAGLAYDPERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILTSVEKILQEVESL
KRVGKDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPAQIADMILHDQADLGIATEGISGDKSLVS
LPGYQWQHMVITPPDHPLLEKKHLTLEDLATFPLITYDVNFAGRPKIDKAFELRHLQPDIVLEAIDADVIKTYVEVGLGV
GIVAGLAYDPERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL
>Mature_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILTSVEKILQEVESL
KRVGKDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPAQIADMILHDQADLGIATEGISGDKSLVS
LPGYQWQHMVITPPDHPLLEKKHLTLEDLATFPLITYDVNFAGRPKIDKAFELRHLQPDIVLEAIDADVIKTYVEVGLGV
GIVAGLAYDPERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=45.2459016393443, Blast_Score=290, Evalue=7e-80,
Organism=Escherichia coli, GI1788296, Length=310, Percent_Identity=45.4838709677419, Blast_Score=283, Evalue=8e-78,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=33.8028169014084, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693105, Length=264, Percent_Identity=26.5151515151515, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788481, Length=248, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=27.9352226720648, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082132, Length=300, Percent_Identity=24.6666666666667, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789204, Length=235, Percent_Identity=26.3829787234043, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=23.3606557377049, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=22.65625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=29.5302013422819, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787879, Length=235, Percent_Identity=26.8085106382979, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=270, Percent_Identity=23.7037037037037, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=239, Percent_Identity=25.5230125523013, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081904, Length=270, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=239, Percent_Identity=26.3598326359833, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35170; Mature: 35170

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHH
EPGRRILTSVEKILQEVESLKRVGKDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGNPAQIADMILHDQADLGIATEGISGDKSLVSLPGYQWQHMVITPPDHPLLE
EEEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCC
KKHLTLEDLATFPLITYDVNFAGRPKIDKAFELRHLQPDIVLEAIDADVIKTYVEVGLGV
CCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH
GIVAGLAYDPERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
EQAMSGDHEAYEL
HHHHCCCCHHHCC
>Mature Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHH
EPGRRILTSVEKILQEVESLKRVGKDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGNPAQIADMILHDQADLGIATEGISGDKSLVSLPGYQWQHMVITPPDHPLLE
EEEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCC
KKHLTLEDLATFPLITYDVNFAGRPKIDKAFELRHLQPDIVLEAIDADVIKTYVEVGLGV
CCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH
GIVAGLAYDPERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
EQAMSGDHEAYEL
HHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]