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The map label for this gene is btuF [H]

Identifier: 94311714

GI number: 94311714

Start: 3029385

End: 3030287

Strand: Direct

Name: btuF [H]

Synonym: Rmet_2782

Alternate gene names: 94311714

Gene position: 3029385-3030287 (Clockwise)

Preceding gene: 94311713

Following gene: 94311722

Centisome position: 77.12

GC content: 66.89

Gene sequence:

>903_bases
ATGAAAGCCTTCTACGTAGCGAGTGCAGCAAGTGCCCTGCTGATTGCCGCCATGCCGGCGCATGCCGCGGTCTCCGTGGT
CGACGATGCGGGCGAGACCGTCACGCTGGCGCAGCCGGCACGCCGCATCGTCTCTCTGGCGCCGCACGTGACCGAACTGA
TCTTCGCAGCGGGCGGCGGCGACCGCATCGTCGGCACAGTCAGCTACAGCGACTACCCGCCGCAGGCGCGCGATATTCCG
CGCGTGGGTGACAACAAGGCGCTCGATCTCGAACGGATCGCCGCGCTCAAACCCGACCTCATCGTGGTCTGGCGCCACGG
TAACGCGCAGAAGCAGACCGACCGCCTGCGCGCGCTCGGCATGCCGCTGTTCTTCAGCGAGCCACGCAAGCTCGATGGCA
TCGCCGACAATCTGGAAAAGCTCGGCACGCTGATGGGCACCACGCCCGTGGCCCACCGCGCCGCTACCGATTTCCGCCAG
CAGGTAAGCACGCTGCGCAAAACCTACGCGGGCCGCCCGCCCGTGACCGTGTTCTATCAGGTCTGGCAGCAGCCGCTGAT
GACGCTGAACGGCGAGCACATGGTCAGCGACCTGCTCGCGCTATGCGGCGGTCGCAACCTGTTCGGGAAGGAAGCGGCAC
TGGTGCCAACGGTCTCGGTCGAAGCCGTGGTCGCGGGCAACCCCGAGGTCATGCTGACCGCCGGTATGGGCGCCACGCGA
TCCGACAAGCCGCTCGCGGACTTTGCGATGTGGGAGAAGTGGAAGCAGGTTACGGCCGTGGCACGCAACAACCTGTTCGT
GATTGACGGCGATCTGGTCAATCGCGCTGGCCCGCGCGTGGCCCAGGGCGCGGCGGAGATTTGCAAGGACCTGGACGTGG
CGAGGTCACGGCGGCCAGGCTGA

Upstream 100 bases:

>100_bases
GCGCTTTGGCTTGCCACCTAACGACGACGCGGCATGGCAACGGCTGGATGCCGCGCTTGGCCACGCACGCCACGCACTCC
CTTCCCTGACCTGAATTCCG

Downstream 100 bases:

>100_bases
TGCTGGCGCGCTACGCCTTGGCGCTCCGGTTCCACCACCCCAGTCGGGCCTGCCGCTTCCCCAGGTGGAAGTGACAGGTC
GCCCCGAACTCAAGCTCCCA

Product: vitamin B12 ABC transporter periplasmic-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGGDRIVGTVSYSDYPPQARDIP
RVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGMPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQ
QVSTLRKTYAGRPPVTVFYQVWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATR
SDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG

Sequences:

>Translated_300_residues
MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGGDRIVGTVSYSDYPPQARDIP
RVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGMPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQ
QVSTLRKTYAGRPPVTVFYQVWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATR
SDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG
>Mature_300_residues
MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGGDRIVGTVSYSDYPPQARDIP
RVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGMPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQ
QVSTLRKTYAGRPPVTVFYQVWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATR
SDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=34.5238095238095, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32262; Mature: 32262

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGG
CCEEEEHHHHHHHHHHHCCCHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCC
DRIVGTVSYSDYPPQARDIPRVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALG
CEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
MPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQQVSTLRKTYAGRPPVTVFYQ
CCCEECCCCHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECEEEEEEEECCCCEEEEECCCCCC
SDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGG
CCEEEEHHHHHHHHHHHCCCHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCC
DRIVGTVSYSDYPPQARDIPRVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALG
CEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
MPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQQVSTLRKTYAGRPPVTVFYQ
CCCEECCCCHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECEEEEEEEECCCCEEEEECCCCCC
SDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA