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The map label for this gene is cobD [H]

Identifier: 94311712

GI number: 94311712

Start: 3027370

End: 3028350

Strand: Direct

Name: cobD [H]

Synonym: Rmet_2780

Alternate gene names: 94311712

Gene position: 3027370-3028350 (Clockwise)

Preceding gene: 94311711

Following gene: 94311713

Centisome position: 77.07

GC content: 71.66

Gene sequence:

>981_bases
ATGATCATGCTGTCCTGGCCGGCCTGCGTGGCGGCTGCGATCGCCGGCGTTCTGCTGGATCGCTGGCTGGGCGAGCCGCG
CCGCTGGCATCCGCTGGTCGGCTTTGGCCGCTTGGCCACGGCCGTCGCCAATCGCCTCAATCGTCCCGAACGTGGCGCGC
TACGCCAGCGCGTGGCGGGCCTGATTGCGTGGGCGGTGGTCGTGCTCGTGCCGGCCGCCATCGCGGTGTGGCTGATCTCG
CTGGCGCAGGCCGTGCATCCCGCGCTGGCCTGGGTGCTCCATGCAGTGGCGCTCTATGCGGCGCTTGGCGCCCGCAGCCT
CTCTCAACACATCGCTCCGATCGCCGGCGCCCTGACACGCGGCGACCTGTACGCCGCGCGCCGGCTTACCGCATGCATCG
TCTCGCGTGACACCGAGGATGCCGACGCCGAGGCGCTGGCCCGCGCCGCGTGCGAATCGGCGCTGGAGAACGGCAACGAT
GCGATCTTCGGCGCGCTGTTCTGGTTCCTGATCGGCGGCGCGCCGGCCGTGATCGTGTTCCGTCTGGCCAATACGCTGGA
CGCGATGTGGGGCTATCGCACGCCACGCTGGCTGATGTTCGGCTGGGCGGCGGCACGCATCGACGACGTACTCAATTTCG
TTCCGGCGCGACTGACCGCGCTCTCCTATGCATCGCTCGGCGCAACGGCGCAGGCGCTGCGCTGCTGGCGCACCCAGGCA
CGCGCGTGGTCGAGCCCCAATGCCGGTCCGGTGATGGCGGCCGGCGCGGGCGCCGTGGGCGTGGCTTTGGGCGGCGGCGC
GCGCTATCACGGCGAGTGGGAAGAGCGACCGCCGCTCGGCGCCGGTGACGCGCCCGCGGCCATCCACATCCGCGCCTGTC
TTCGGCTGGTGCAGCGCGCGGTGTGGCTGTGGCTCGGTCTGGCGATGCTGAGCGTGCCGCTGGTCGACCTGCTCGCCGGA
CTGCTGGAGCGCACGCCATGA

Upstream 100 bases:

>100_bases
ACGAGCTTGGCCGCCTGAACCAGAAGCTCGCCGCGGCCGCCGATCGCGTGCGTCTGCTTGTGGCCGGCATTCCCGTCGCC
GTCAAGGACGCGCCCATCGC

Downstream 100 bases:

>100_bases
CCCACGCACCGATCCGCCACGGCGGCAACCTGCTCGCCGCCGTGCGCCGCTATGGCCGCCCGGCGGAAACGTGGCTCGAC
CTGTCGACCGGCATCAATCC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAGLIAWAVVVLVPAAIAVWLIS
LAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTRGDLYAARRLTACIVSRDTEDADAEALARAACESALENGND
AIFGALFWFLIGGAPAVIVFRLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQA
RAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRAVWLWLGLAMLSVPLVDLLAG
LLERTP

Sequences:

>Translated_326_residues
MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAGLIAWAVVVLVPAAIAVWLIS
LAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTRGDLYAARRLTACIVSRDTEDADAEALARAACESALENGND
AIFGALFWFLIGGAPAVIVFRLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQA
RAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRAVWLWLGLAMLSVPLVDLLAG
LLERTP
>Mature_326_residues
MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAGLIAWAVVVLVPAAIAVWLIS
LAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTRGDLYAARRLTACIVSRDTEDADAEALARAACESALENGND
AIFGALFWFLIGGAPAVIVFRLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQA
RAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRAVWLWLGLAMLSVPLVDLLAG
LLERTP

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34714; Mature: 34714

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAG
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIAWAVVVLVPAAIAVWLISLAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLYAARRLTACIVSRDTEDADAEALARAACESALENGNDAIFGALFWFLIGGAPAVIVF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRA
HCCCCCCCCCEEECCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VWLWLGLAMLSVPLVDLLAGLLERTP
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAG
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIAWAVVVLVPAAIAVWLISLAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLYAARRLTACIVSRDTEDADAEALARAACESALENGNDAIFGALFWFLIGGAPAVIVF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRA
HCCCCCCCCCEEECCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VWLWLGLAMLSVPLVDLLAGLLERTP
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]