BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is paaG1 [H]

Identifier: 94311539

GI number: 94311539

Start: 2830824

End: 2831615

Strand: Direct

Name: paaG1 [H]

Synonym: Rmet_2603

Alternate gene names: 94311539

Gene position: 2830824-2831615 (Clockwise)

Preceding gene: 94311538

Following gene: 94311540

Centisome position: 72.07

GC content: 65.4

Gene sequence:

>792_bases
ATGCAATTCGAAACCCTGTTGTACGAAGTGGTCGACGGCGTGGCCATCATCCGGCTAAACCGCCCGGATCGCATGAACAC
ACTCGGCGGCACCATGAAACCCGATCTCGCCCGCGCGTTCTTCGAGTTGGCCCGTGATGACACCAGCGTTCGCTGTGTAG
TGCTGACCGGCACCGGCGATAGAGCATTCTGCGCGGGCGCCGACATCAAGGAACGCGCCGGCGGCACGGCCCACCCCGCC
GACTACTACGTCACGCAGCAACGCACGCACGAGCTGTTCCGTGGCATCGAGGAATTCGAGAAACCGGTGATCGCCGCCAT
CAATGGCGTAGCGCTCGGCGGCGGCCTGGAACTGGCCCTGTGCGCCGACATCCGCCTGGCCGCCAGATCGGCACGATTCG
GCCTGCCAGAGATCAAGCTCGGCGTCATTCCCGCGGCGGGCGGCACGCAGCGCCTGCCGCGCGTGGTGGGTCCGGCCATC
GCCAAGGAACTGATCTTTACCAGCGAACTGATCAACGCCGACGCCGCGAAAGACATCCGCCTGGTGAACCGTGTGATCGA
CGACGACAAGCTGATGGATGCGGCACTGACGATGGCCAGGCGCATCGCCGCGCAACCACCGCTGGCCGTGCGCTTCGCCA
AGCGGTCGGTCAACCTGGGCATGCAGGGCTCGGTCGACATGGGCCTGCAGTTCGAATGCTACGGCGCGGCCATGGTGATG
GACAGCGAGGACAGGAAGGAAGGGATGCGCGCATTCGTCGAGAAGCGCGAGCCCCGGTTCGTCGGGCGCTAG

Upstream 100 bases:

>100_bases
GCGCACATCCTGGCAGCATCGCACTGACCGTGCGGATGCGCCTGCCGCGAGCACGGCTGGCGCATCCGTCGCGCACATCA
TTCCAGGCAAGGAGACAAGG

Downstream 100 bases:

>100_bases
TCCGCACGCGAACGGCATCGAAACACGGAACATCGAAAACTGGAGGAGTACGGAATGGAACTGCATCTGCAAGGCAAGGT
GGCATTGGTAACTGGAGGCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGDRAFCAGADIKERAGGTAHPA
DYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELALCADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAI
AKELIFTSELINADAAKDIRLVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVM
DSEDRKEGMRAFVEKREPRFVGR

Sequences:

>Translated_263_residues
MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGDRAFCAGADIKERAGGTAHPA
DYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELALCADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAI
AKELIFTSELINADAAKDIRLVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVM
DSEDRKEGMRAFVEKREPRFVGR
>Mature_263_residues
MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGDRAFCAGADIKERAGGTAHPA
DYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELALCADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAI
AKELIFTSELINADAAKDIRLVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVM
DSEDRKEGMRAFVEKREPRFVGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=40.5622489959839, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=41.2213740458015, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI150378541, Length=256, Percent_Identity=34.375, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=32.03125, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=37.8378378378378, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=34.7826086956522, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=28.4584980237154, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=29.6803652968037, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=29.6803652968037, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=29.6803652968037, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=163, Percent_Identity=30.0613496932515, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI37594471, Length=163, Percent_Identity=30.0613496932515, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI261878539, Length=109, Percent_Identity=38.5321100917431, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI62530384, Length=205, Percent_Identity=27.8048780487805, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=40.2390438247012, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=39.5437262357414, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI221142681, Length=254, Percent_Identity=37.4015748031496, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=32.7067669172932, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=32.6923076923077, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788682, Length=206, Percent_Identity=36.4077669902913, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=35.2331606217617, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=40.4761904761905, Blast_Score=177, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=41.2, Blast_Score=176, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=43.6123348017621, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560910, Length=250, Percent_Identity=30.8, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=29.2307692307692, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=27.3764258555133, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=232, Percent_Identity=34.051724137931, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=232, Percent_Identity=34.051724137931, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=232, Percent_Identity=34.051724137931, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=34.4632768361582, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=30.9090909090909, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=266, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=30.5164319248826, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=155, Percent_Identity=30.3225806451613, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=200, Percent_Identity=27.5, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=44.140625, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=40.3225806451613, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=40.3225806451613, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=30.2788844621514, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21357171, Length=214, Percent_Identity=31.7757009345794, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650670, Length=253, Percent_Identity=30.8300395256917, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571729, Length=144, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571730, Length=144, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=35.4838709677419, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583077, Length=186, Percent_Identity=35.4838709677419, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583079, Length=186, Percent_Identity=35.4838709677419, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=231, Percent_Identity=24.6753246753247, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=24.4094488188976, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28684; Mature: 28684

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGD
CCHHHHHHHHHCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCC
RAFCAGADIKERAGGTAHPADYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELAL
CEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCEEEE
CADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAIAKELIFTSELINADAAKDIR
EHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVM
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCCCCCCCCCCEEEEEECEEEEE
DSEDRKEGMRAFVEKREPRFVGR
CCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGD
CCHHHHHHHHHCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCC
RAFCAGADIKERAGGTAHPADYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELAL
CEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCEEEE
CADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAIAKELIFTSELINADAAKDIR
EHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVM
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCCCCCCCCCCEEEEEECEEEEE
DSEDRKEGMRAFVEKREPRFVGR
CCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]