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The map label for this gene is yagI [H]

Identifier: 94311536

GI number: 94311536

Start: 2828218

End: 2828967

Strand: Reverse

Name: yagI [H]

Synonym: Rmet_2600

Alternate gene names: 94311536

Gene position: 2828967-2828218 (Counterclockwise)

Preceding gene: 94311542

Following gene: 94311535

Centisome position: 72.02

GC content: 67.33

Gene sequence:

>750_bases
ATGCCTAGCTTTGTTCCTGCTGCCGCGCGCAGCCTTGCCGTCTTCGAGGTGTTCTCGCGCGAGAAGCGCGAGCTCTCCAA
TTCGGAACTTGCGCGCCTGCTCGACCTGCCGGAGAGCAGTTGCTCGGACCTGCTGCATACGCTGACCGAGACGGGGTACC
TGATGCGTACCGCGCGCACGCGCCGCTTCTATCCCACGATGCGGCTCCATTCGATCTGCGAGGCCATTGCGGGCAACGAT
CCGCTGGCCGCCGTGGCGGCCGAGGCCACGGAGTTCCTCACTGGCAAGACGGGCGAGACCTCGCTATGCGGCCGGCTGGA
TGGCGCCTACGTGCGGGTGACTGGCTTCCAGGAAAGTCGCTACGAACTGCGTTACGTACTGCGCGTTGGCAAGCGCCTGG
CCTTGCACGCCACGGCGCTCGGCAAGGCGTTGCTGGCGGTGCTGCCGCCGGAAGACGCCGCGCGGCAGTTGCGCCTGCGC
GCGCTGCCCAAGCTGGGGCCGAAGACGGAAGTCAATCCGGTCGCGCTGGAAAAGCAGGTCGCCGAAGTGCGCGAGCGCGG
CTGGGCGTGGGTCGAGGACGAGGGAGGAGAAGGGGTGACGGCCATCGCGGTGGCCGGGCGGATCGGCGACGAGCCGTTCG
CGATCTCGTTGGCGGGCCCCACCGAACGATTCAATCGCCGCAAGGACGAGTACCTGGAGGTGATCGAGGCAGCGCGGGAT
CTGGTTTTCCCGACCGCCCAGAAGGATTAG

Upstream 100 bases:

>100_bases
TAGTCATGTCACCTGTCAAACACGGAAACGCGTACGAAATCTCGTAGACTGTGCGGTTTGGGCGGATGTTGCCCTTTCAA
TACGAGATTTCGGAGAACCC

Downstream 100 bases:

>100_bases
GCCGGCACATGGGACCGCTCCTGTGGCCGGCTTTGTTTCGACGGTGCCTGGCGATGATGCCGCTGGCGCCGGATGCATTT
CCCAACCGGACTATTACAGG

Product: regulatory protein, IclR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTARTRRFYPTMRLHSICEAIAGND
PLAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESRYELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLR
ALPKLGPKTEVNPVALEKQVAEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARD
LVFPTAQKD

Sequences:

>Translated_249_residues
MPSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTARTRRFYPTMRLHSICEAIAGND
PLAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESRYELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLR
ALPKLGPKTEVNPVALEKQVAEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARD
LVFPTAQKD
>Mature_248_residues
PSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTARTRRFYPTMRLHSICEAIAGNDP
LAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESRYELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLRA
LPKLGPKTEVNPVALEKQVAEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARDL
VFPTAQKD

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=205, Percent_Identity=28.2926829268293, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082359, Length=247, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788131, Length=240, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786715, Length=236, Percent_Identity=26.6949152542373, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 27427; Mature: 27295

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTART
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RRFYPTMRLHSICEAIAGNDPLAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESR
HHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCHHH
YELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLRALPKLGPKTEVNPVALEKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARD
HHHHHCCCEEEECCCCCCEEEEEEECCCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHH
LVFPTAQKD
HCCCCCCCC
>Mature Secondary Structure 
PSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTART
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RRFYPTMRLHSICEAIAGNDPLAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESR
HHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCHHH
YELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLRALPKLGPKTEVNPVALEKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARD
HHHHHCCCEEEECCCCCCEEEEEEECCCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHH
LVFPTAQKD
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]