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The map label for this gene is yjbJ [H]

Identifier: 94311456

GI number: 94311456

Start: 2737532

End: 2738374

Strand: Reverse

Name: yjbJ [H]

Synonym: Rmet_2520

Alternate gene names: 94311456

Gene position: 2738374-2737532 (Counterclockwise)

Preceding gene: 94311457

Following gene: 94311455

Centisome position: 69.71

GC content: 65.95

Gene sequence:

>843_bases
ATGAGGCAGGGCAGCAACAACATCGGACGATTCGATCGATTCCGCCGATCTGGGCGAATGGTGGCGCGGGTGGCAGCGCG
GGTGGCAGGAATGCTTGCCGCGCTGGCGTCGCTCTGCCTTGCCGCGCCTGCCCATGCGGAGCTCTGGGGCTTTATCGACG
CCGATGGCGTGGCGCACTTTGCCGATGCGAAGCTCGATGCGCGCTACAAGCTGTTCATGAAGGATGGCGGCAGCTTCGAT
TCGAGCAACATGCGCAATCTGCCCCGGGGTGGCCTGCCCGGCGGCAAGCTGGACCCCGAACGCGAGAAGCTGTACCGCTA
CGTGCTCAACCATCCGAACCTGGCGATGGTCGATCCGATCATCCAGAAGGCGGCTGCGGCGCAGAACGTGGACCCAGCAC
TGGTCAAGGCCGTGATGGCCGTGGAGAGCGGCTTCAATCCGACGGCGGTGTCGCCGAAGGGCGCGATCGGGCTGATGCAG
GTGATTCCGGACACGGGCGCGCGCTTCGGCATCGCCGCAGACAAGCGCGGGACCGTGGAGCAGAAACTGGCCGATCCACG
TCTGAACATCACCACCGGTGTGCGGTACCTGCGCTGGCTGATGGAGCTGTTCCCGAACAACCTGGAGCTGGTGCTGGCCG
CTTACAACGCCGGTGAGGGCGCCGTGCAACGCTACAACAACCAGATCCCGCCATATCCCGAGACGCAGCAGTATGTCGCC
ACGGTGCTGGAGTTCTATCGCCTGTACCAGCCGGGCGAGGCGTCCGAGGCGCGCGTGGTGCGGACCGGCATGGCGGCCGA
GCGGGTCAGGATGGTGATCGGCGGTCGCCGGCCCATGCCCTGA

Upstream 100 bases:

>100_bases
GATGAACATGCTGATGGGCAAGGGCGAGGCCTCGCAACGACGCAGCTGGCTGGAAGAGAAGGGCAACGAAGTGGAGGCCG
ACATCTGACGGCGATGCGCA

Downstream 100 bases:

>100_bases
CATGCGGGCGCCTGATGCCGCTTTCTGAGATGGATTTGCCACGCGGCCAGAGGTTGCGGCGGTATCGCCATCTACTTCCT
TTAGAATCCCACGCTGACTG

Product: putative lytic transglycosylase

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRQGSNNIGRFDRFRRSGRMVARVAARVAGMLAALASLCLAAPAHAELWGFIDADGVAHFADAKLDARYKLFMKDGGSFD
SSNMRNLPRGGLPGGKLDPEREKLYRYVLNHPNLAMVDPIIQKAAAAQNVDPALVKAVMAVESGFNPTAVSPKGAIGLMQ
VIPDTGARFGIAADKRGTVEQKLADPRLNITTGVRYLRWLMELFPNNLELVLAAYNAGEGAVQRYNNQIPPYPETQQYVA
TVLEFYRLYQPGEASEARVVRTGMAAERVRMVIGGRRPMP

Sequences:

>Translated_280_residues
MRQGSNNIGRFDRFRRSGRMVARVAARVAGMLAALASLCLAAPAHAELWGFIDADGVAHFADAKLDARYKLFMKDGGSFD
SSNMRNLPRGGLPGGKLDPEREKLYRYVLNHPNLAMVDPIIQKAAAAQNVDPALVKAVMAVESGFNPTAVSPKGAIGLMQ
VIPDTGARFGIAADKRGTVEQKLADPRLNITTGVRYLRWLMELFPNNLELVLAAYNAGEGAVQRYNNQIPPYPETQQYVA
TVLEFYRLYQPGEASEARVVRTGMAAERVRMVIGGRRPMP
>Mature_280_residues
MRQGSNNIGRFDRFRRSGRMVARVAARVAGMLAALASLCLAAPAHAELWGFIDADGVAHFADAKLDARYKLFMKDGGSFD
SSNMRNLPRGGLPGGKLDPEREKLYRYVLNHPNLAMVDPIIQKAAAAQNVDPALVKAVMAVESGFNPTAVSPKGAIGLMQ
VIPDTGARFGIAADKRGTVEQKLADPRLNITTGVRYLRWLMELFPNNLELVLAAYNAGEGAVQRYNNQIPPYPETQQYVA
TVLEFYRLYQPGEASEARVVRTGMAAERVRMVIGGRRPMP

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=123, Percent_Identity=38.2113821138211, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082441, Length=166, Percent_Identity=30.7228915662651, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87081855, Length=92, Percent_Identity=42.3913043478261, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 30660; Mature: 30660

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQGSNNIGRFDRFRRSGRMVARVAARVAGMLAALASLCLAAPAHAELWGFIDADGVAHF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHH
ADAKLDARYKLFMKDGGSFDSSNMRNLPRGGLPGGKLDPEREKLYRYVLNHPNLAMVDPI
HCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
IQKAAAAQNVDPALVKAVMAVESGFNPTAVSPKGAIGLMQVIPDTGARFGIAADKRGTVE
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHH
QKLADPRLNITTGVRYLRWLMELFPNNLELVLAAYNAGEGAVQRYNNQIPPYPETQQYVA
HHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
TVLEFYRLYQPGEASEARVVRTGMAAERVRMVIGGRRPMP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRQGSNNIGRFDRFRRSGRMVARVAARVAGMLAALASLCLAAPAHAELWGFIDADGVAHF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHH
ADAKLDARYKLFMKDGGSFDSSNMRNLPRGGLPGGKLDPEREKLYRYVLNHPNLAMVDPI
HCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
IQKAAAAQNVDPALVKAVMAVESGFNPTAVSPKGAIGLMQVIPDTGARFGIAADKRGTVE
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHH
QKLADPRLNITTGVRYLRWLMELFPNNLELVLAAYNAGEGAVQRYNNQIPPYPETQQYVA
HHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
TVLEFYRLYQPGEASEARVVRTGMAAERVRMVIGGRRPMP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]