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The map label for this gene is rpoE [H]

Identifier: 94311361

GI number: 94311361

Start: 2635319

End: 2635918

Strand: Reverse

Name: rpoE [H]

Synonym: Rmet_2425

Alternate gene names: 94311361

Gene position: 2635918-2635319 (Counterclockwise)

Preceding gene: 94311362

Following gene: 94311360

Centisome position: 67.1

GC content: 61.17

Gene sequence:

>600_bases
GTGAGTGAACGCGAAGCCGATCAGCTTCTAGTTGAACGCGTCCAGCAGGGCGACAAACGGGCCTTTGAGTTGCTGGTGAC
CAAATATCACCGCAAGATCATCCGCCTGATCTCCCGCCTTGTCCGAGACCCCGCCGAAGTGGAGGATGTGGCGCAAGATG
CCTTTATCAAGGCATATCGCGCGCTGCCCCAGTTCCGGGGCGAATCCGCTTTCTACACGTGGCTGTACCGGATCGCCGTC
AACACGGCCAAGAACTACCTGGCCACCCAGGGACGGCGGCCGGAAGCGTCCAGCGATATCGATGCCGAAGAGGCTGAAAC
TTTTGCGGACGGTGAGCAACTAAGGGATATCAATACGCCGGAGTCGATGCTCCACACGCGCCAGGTTGCCGATACGGTGA
ACCGCGCCATGGAGGCATTGCCGGAAGAATTACGCACCGCCATTACGCTGCGCGAGATCGAAGGCCTCAGCTATGAGGAG
ATCGCGGAGGCGATGGGGTGTCCGATCGGTACGGTGCGCTCGCGGATTTTCCGGGCGCGCGAAGCGATCGCCGAGAAGTT
GCGCCCGCTGCTGGGAACGGCAGAGGGCAAGCGGTGGTAG

Upstream 100 bases:

>100_bases
ATGCGCTCCGCGACACCCGAAATCGCCTGGATCGTAGGTAAAATACAACGCTTGTCTTGACGACGAAACCAAGAGATACA
CTGCAGCAGGTGAACTATTC

Downstream 100 bases:

>100_bases
GGGATGTCAGCAGGATGCTGGTGTCTCTCAGTTTGAATGTCGTGCAGCCAGCGTTGCAGCCTATTCCTGCATCGATAAAC
GACAAGCAGTAGTGACGGGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTPESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEE
IAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW

Sequences:

>Translated_199_residues
MSEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTPESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEE
IAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW
>Mature_198_residues
SEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVN
TAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTPESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEEI
AEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=62.82722513089, Blast_Score=243, Evalue=5e-66,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22755; Mature: 22624

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHCCCCH
ESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEEIAEAMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAEKLRPLLGTAEGKRW
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHCCCCH
ESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEEIAEAMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAEKLRPLLGTAEGKRW
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]