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The map label for this gene is lpdA [H]

Identifier: 94311334

GI number: 94311334

Start: 2608581

End: 2609957

Strand: Direct

Name: lpdA [H]

Synonym: Rmet_2398

Alternate gene names: 94311334

Gene position: 2608581-2609957 (Clockwise)

Preceding gene: 94311333

Following gene: 94311335

Centisome position: 66.41

GC content: 67.39

Gene sequence:

>1377_bases
ATGCAGACAAGGAAAGTAGATGTCGCCATCATTGGCGCGGGAACCGCTGGCAGCACCGCATTCCATGCGCTGAAAGCGAC
AGGCAAACAGGTGGTTCTGATCGATCGGGGGCCATTGGGGACGACCTGCGCGCGCGTCGGATGCATGCCCTCGAAGGCGG
CCCTGCATGCCGGCATGCACTGGAAGGTCGCCAAGGCACTGGCAGCGAGCGCCACGCCATCGACCCAAGGGCCGCAGGCC
TTATGGTCCCACGCCCGCCAGACCAGGGATGCGCTGGCCGGCGCCGCCGCGGAGCGCACGCGCAAGGCGGCTGGCGAGAG
CCTGATCATGGCCGAAGCGCGCTTCGTCGCGCCGGGCATCTTGCAGGCAGGTGAACAACGGATCGAGGCCAGCGCCTTCA
TCGTGGCGACGGGCTCTCGCCCGGTGGTCCCCAAAGCGCTCGACGGCGTTGCCTCCCGCATCCTGACCACCGATACCCTG
TTCGACCTGGGCGTCCTGCCCGCACGGATGGGAATCCTGGGACTGGGCGCCATCGGGCTGGAACTCGGCCTGGCCCTGTC
TCGCTTGGATGTGCAGGTGGTCGCGGCAGACCAGAAAGACGCCATCGGCGGCATCCAGGACCCCGATGTCCTGGAGCGGG
CGATCGCCAGGTTCGAGACCGAGTTGCCGATGTGGTTGGGCGCCGCCGTCGAAGTCAGCCTTGAAGGCGACCAGGTGCGC
ATGCGCGCCGGCGAGCGCGACGCGCTCGTGGATCGGCTTCTCGTCGTGACGGGACGACAACCCAATACCGAGGCGCTCGA
TCTCGCCGCGGCCGGCATCTCCTTGGATCAGGCTGGCCGACCGAGCATCGACCCCGCGACCATGCAGGCCTGCGGGCCGT
CCCAGGTGCCGATTTTCTTCGCAGGCGATGTGCAGCCGGATCGCCCTCTGATGCACGAGGCCGCGGACGAGGGTCAGATG
GCAGCGCAAGCCGCACTGGCGAGCTTGCGCGGCGAAAGTTGGCCGGGCGCATCCCGGCGCGTGCCGATCACGATCCTGTT
CACCGACCCGGATGCGTGCGCAGTCGGCATGACCTACGAGGCGGCGGTGCAGGAAGGCGCGGTCGTCGGCACCGCGGAAG
GCAGCGGCAACGGGCGCTCCAAGATTCTTGGTGCTCCGGAAAACCTGCTGCACATCTATGCCGACCCCGGCTCAGGCGCC
TTGCTCGGTGCCAGCATGCTCCTGACGCAAGGCGAGCACCTGGCGCACCTGATCGCTTGGGCGATCCAGGCGAAGCAGAC
CGTCAACGATCTGCTGGCCATGCCGTACTACCACCCCTCCATCGAGGAGATGCTGCAAAGCGCCTTGAAATCCGCATCCC
AGCAGATGCGCCCGTGA

Upstream 100 bases:

>100_bases
GTTTTCGATCACCTGCGTGGCCGTGGCGGCCCTCATGCTCATGCGCAATGTTGGCTAGAGGTCCGATGTTCGATTAATTG
ATTGTGGACGGGAAAATTCA

Downstream 100 bases:

>100_bases
ACAACCCTTCTGACTGCACCACCGACCGACCATGACCCAAGACACCCAGCGCCCCACATTGAAAGTGATCCTCCACGCAC
CGACGGCAGAAGCCTTTGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMHWKVAKALAASATPSTQGPQA
LWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGILQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTL
FDLGVLPARMGILGLGAIGLELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVR
MRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFFAGDVQPDRPLMHEAADEGQM
AAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYEAAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGA
LLGASMLLTQGEHLAHLIAWAIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP

Sequences:

>Translated_458_residues
MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMHWKVAKALAASATPSTQGPQA
LWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGILQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTL
FDLGVLPARMGILGLGAIGLELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVR
MRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFFAGDVQPDRPLMHEAADEGQM
AAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYEAAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGA
LLGASMLLTQGEHLAHLIAWAIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP
>Mature_458_residues
MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMHWKVAKALAASATPSTQGPQA
LWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGILQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTL
FDLGVLPARMGILGLGAIGLELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVR
MRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFFAGDVQPDRPLMHEAADEGQM
AAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYEAAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGA
LLGASMLLTQGEHLAHLIAWAIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=26.4270613107822, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI50301238, Length=361, Percent_Identity=25.7617728531856, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=27.6086956521739, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082354, Length=481, Percent_Identity=23.9085239085239, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=24.1228070175439, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789915, Length=264, Percent_Identity=26.8939393939394, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=27.6729559748428, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=349, Percent_Identity=24.0687679083095, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71983419, Length=349, Percent_Identity=24.0687679083095, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=25.979381443299, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=27.2340425531915, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47692; Mature: 47692

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMH
CCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHCCCH
WKVAKALAASATPSTQGPQALWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHH
LQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTLFDLGVLPARMGILGLGAIGL
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVR
HHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCCEEE
MRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFF
ECCCCHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEE
AGDVQPDRPLMHEAADEGQMAAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYE
ECCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEECCCHH
AAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGALLGASMLLTQGEHLAHLIAW
HHHHCCCEEEECCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
AIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMH
CCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHCCCH
WKVAKALAASATPSTQGPQALWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHH
LQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTLFDLGVLPARMGILGLGAIGL
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVR
HHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCCEEE
MRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFF
ECCCCHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEE
AGDVQPDRPLMHEAADEGQMAAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYE
ECCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEECCCHH
AAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGALLGASMLLTQGEHLAHLIAW
HHHHCCCEEEECCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
AIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11016950 [H]