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The map label for this gene is fumA [H]

Identifier: 94311211

GI number: 94311211

Start: 2495497

End: 2497062

Strand: Direct

Name: fumA [H]

Synonym: Rmet_2273

Alternate gene names: 94311211

Gene position: 2495497-2497062 (Clockwise)

Preceding gene: 94311210

Following gene: 94311212

Centisome position: 63.53

GC content: 64.24

Gene sequence:

>1566_bases
ATGATTCCGGGCTGTGTTTCACCGCTGCCCATCCCCCACAAAATGACAGTCATCAAGCAAGAAGACCTCATCCAGAGCGT
CGCCGATTCCCTGCAGTACATCAGCTACTACCATCCGATGGACTACATCACCAGCCTGGGCCGCGCCTATGAGCTGGAAA
AGAGTCCGGCCGCCAAGGATGCGATCGCGCAGATCCTGACCAACAGCCGCATGTGCGCCGAAGGCAAGCGCCCGCTGTGC
CAGGACACCGGCATCGTGACGGTCTTCCTGAAGGTGGGCATGAACGTGCGCTGGGATGGCGCCACGATGAGCCTGGAAGA
CATGGTCAACGAAGGCGTGCGCCGCGCGTACAACGACGTGGACAACAAGCTGCGCGCCAGCGTGCTGGCCGATCCGGCCG
GCAAGCGCACCAATACGAAGGACAACACCCCGGCGGTGATCAACATGTCGATCGTTCCGGGCGATACGGTGGACGTGATC
GTCGCAGCCAAGGGCGGCGGTTCCGAAGCCAAGTCGAAGTTCGTGATGCTCAACCCGTCTGACTCGATCGTCGACTGGGT
GCTCAAGACTGTCCCGACCATGGGCGCCGGCTGGTGCCCGCCGGGCATGCTGGGCATCGGCATTGGCGGCACGGCTGAAA
AGGCCATGCTGCTGGCCAAGGAAGCCCTGATGGAGCCGATCGACATTCAGGACCTGCTCGCCCGCGGCCCGAGCAACCGC
GCCGAAGAACTGCGCATCGAGCTGTACGAGAAGGTCAACGCACTGGGCATCGGCGCGCAGGGCCTGGGCGGCCTGGCTAC
CGTGCTCGACGTGAAGGTGCTGGACTACCCGACCCACGCGGCCAACCTGCCTGTGGCCATGATCCCGAACTGCGCGGCCA
CCCGCCACGCGCACTTCACGCTGGACGGCAGCGGCGTCGCCAAGCTGGAAGCGCCGGACCTGTCGCAATGGCCGAAGGTC
GAGTGGGCACCGAACACCGAAACGTCGAAAAGCGTTGACCTGAACACGCTGACGCCGGAAGAAGTCGCCTCGTGGAAGCC
CGGCCAGACGCTGCTGCTGAACGGCAAGATGCTGACCGGCCGTGATGCCGCGCACAAGCGCATCGCCGACATGCTGGCCA
AGGGCGAGAAGCTGCCGATCGACTTCACGAACCGCGTGATCTACTACGTAGGCCCCGTGGATCCGGTACGTGACGAAGTG
GTTGGCCCGGCTGGCCCCACCACCGCCACCCGCATGGACAAGTTCACCGAGACGATGCTGTCGCAGACTGGCCTGATCGC
GATGATCGGCAAGGCCGAGCGTGGCCCCACCGCAATCGAATCGATCCAGAAGCACAAGTCGGCCTACCTGATGGCCGTGG
GTGGCGCTGCCTACCTCGTGTCGAAGGCGATCCGTGGCGCCAAGGTCCTCGCCTTCGAAGACCTCGGCATGGAAGCCATC
TACGAGTTCGACGTCAAGGACATGCCGGTGACGGTGGCCGTGGATAGCTCGGGCACGTCCGTCCACAAGACCGGCCCCGC
CGAGTGGCAGGCCAAGATCGGCAAGATTCCGGTCGCGACGGCCTAA

Upstream 100 bases:

>100_bases
GGACGGTTGCCCGCCACCCGGGTGACGCCTGCGGCCATCCCGCCACCAAACCTCGTGTCGACGGCTGTATGCAAAGCGGC
AGCCGTCGCCGCGACGGTGT

Downstream 100 bases:

>100_bases
ATCCGGCTACGTGTAGTACGCCCCTCTCCCGCTGCGTGGAAGAGGGGTTGGGGGAGAAGGTGCAGTAGTTCAAATCACGA
CCTGCCGTTGATGGAACAGC

Product: fumarate hydratase class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIPGCVSPLPIPHKMTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEKSPAAKDAIAQILTNSRMCAEGKRPLC
QDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVI
VAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLLARGPSNR
AEELRIELYEKVNALGIGAQGLGGLATVLDVKVLDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKV
EWAPNTETSKSVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEV
VGPAGPTTATRMDKFTETMLSQTGLIAMIGKAERGPTAIESIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAI
YEFDVKDMPVTVAVDSSGTSVHKTGPAEWQAKIGKIPVATA

Sequences:

>Translated_521_residues
MIPGCVSPLPIPHKMTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEKSPAAKDAIAQILTNSRMCAEGKRPLC
QDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVI
VAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLLARGPSNR
AEELRIELYEKVNALGIGAQGLGGLATVLDVKVLDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKV
EWAPNTETSKSVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEV
VGPAGPTTATRMDKFTETMLSQTGLIAMIGKAERGPTAIESIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAI
YEFDVKDMPVTVAVDSSGTSVHKTGPAEWQAKIGKIPVATA
>Mature_521_residues
MIPGCVSPLPIPHKMTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEKSPAAKDAIAQILTNSRMCAEGKRPLC
QDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDVDNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVI
VAAKGGGSEAKSKFVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLLARGPSNR
AEELRIELYEKVNALGIGAQGLGGLATVLDVKVLDYPTHAANLPVAMIPNCAATRHAHFTLDGSGVAKLEAPDLSQWPKV
EWAPNTETSKSVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEV
VGPAGPTTATRMDKFTETMLSQTGLIAMIGKAERGPTAIESIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAI
YEFDVKDMPVTVAVDSSGTSVHKTGPAEWQAKIGKIPVATA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=468, Percent_Identity=31.6239316239316, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=31.6239316239316, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789442, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.9329608938547, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55778; Mature: 55778

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPGCVSPLPIPHKMTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEKSPAAKD
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHH
AIAQILTNSRMCAEGKRPLCQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDV
HHHHHHHCCCHHHCCCCCCCCCCCCEEEEEHHCCCEEECCCCEEHHHHHHHHHHHHHHHH
DNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPS
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCC
DSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLLARGPSNR
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCH
AEELRIELYEKVNALGIGAQGLGGLATVLDVKVLDYPTHAANLPVAMIPNCAATRHAHFT
HHHHHHHHHHHHHHEECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEE
LDGSGVAKLEAPDLSQWPKVEWAPNTETSKSVDLNTLTPEEVASWKPGQTLLLNGKMLTG
ECCCCEEEECCCCCCCCCCCEECCCCCCCCCEECCCCCHHHHHCCCCCCEEEEECEEECC
RDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETML
CHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
SQTGLIAMIGKAERGPTAIESIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAI
HCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEE
YEFDVKDMPVTVAVDSSGTSVHKTGPAEWQAKIGKIPVATA
EEECCCCCCEEEEECCCCCEEECCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPGCVSPLPIPHKMTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEKSPAAKD
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHH
AIAQILTNSRMCAEGKRPLCQDTGIVTVFLKVGMNVRWDGATMSLEDMVNEGVRRAYNDV
HHHHHHHCCCHHHCCCCCCCCCCCCEEEEEHHCCCEEECCCCEEHHHHHHHHHHHHHHHH
DNKLRASVLADPAGKRTNTKDNTPAVINMSIVPGDTVDVIVAAKGGGSEAKSKFVMLNPS
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCC
DSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLLARGPSNR
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCH
AEELRIELYEKVNALGIGAQGLGGLATVLDVKVLDYPTHAANLPVAMIPNCAATRHAHFT
HHHHHHHHHHHHHHEECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEE
LDGSGVAKLEAPDLSQWPKVEWAPNTETSKSVDLNTLTPEEVASWKPGQTLLLNGKMLTG
ECCCCEEEECCCCCCCCCCCEECCCCCCCCCEECCCCCHHHHHCCCCCCEEEEECEEECC
RDAAHKRIADMLAKGEKLPIDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTETML
CHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
SQTGLIAMIGKAERGPTAIESIQKHKSAYLMAVGGAAYLVSKAIRGAKVLAFEDLGMEAI
HCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEECCCCCEE
YEFDVKDMPVTVAVDSSGTSVHKTGPAEWQAKIGKIPVATA
EEECCCCCCEEEEECCCCCEEECCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]