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The map label for this gene is bclA [H]

Identifier: 94311136

GI number: 94311136

Start: 2407206

End: 2408867

Strand: Reverse

Name: bclA [H]

Synonym: Rmet_2198

Alternate gene names: 94311136

Gene position: 2408867-2407206 (Counterclockwise)

Preceding gene: 94311137

Following gene: 94311135

Centisome position: 61.32

GC content: 66.43

Gene sequence:

>1662_bases
ATGGCCGCCCCCCAACCAGCCACCGCTCATGTTGATACCTTCGCACGGGACAGGTTGCCGCCGCCGCAGACGTGGCCGGT
GTTCCAGTTCAATGACGACACGCGCTACCCCGATCGCCTCAATGCGGCAGTGGAGCTTGTGGACCGACACGTGGCGGCCG
GCAACGGTGGCCGCGTGGCGGTGCGCCATGAGCGCGCGGGCCGTATCGAGACCGTGACCTATGCCGAGCTGGCGGCGCTT
GTGGATCGCATCGCGCATGTGTTGGTCGAGGACATGGGGCTGGTGCCGGGCAATCGCGTGCTGCTGCGCGGCCCGAACAA
CCTGATGATGGCGGCGAGCTGGCTTGCCACGCTCAAGGCAGGACTGATCGCCGTGCCGACGATGCCGCTGCTGCGCGCCA
AGGAGCTCAAGCAGATCATCGACAAGGCGCAGGTGACGGCTGCACTCTGCGATGCACGTCTGCGCGACGAGCTGGACGCG
AACCGGCGTGCCGACGGCGAATACTTTTGTCCGACGCTCGAACGCGTGCGCTACTTCCACGGCGAGGGTGCCGATGCGCT
CGAAACCGCGATGGCGGGCAAGCCCGACACGTTCACCGCGTGCGACACGGCCGCTGATGACATCTGCCTGATCGCGTTCA
CGAGTGGTACCACGGGGCAACCCAAGGGCACGATGCATTTCCACCGCGATGTGCTGGCGATGTGCGACCTGTTCTCGCGC
CACGTGCTCAAGCCCACGCCGGACGACGTCTTCTGTGGCACGCCGCCAATCGCATTCACATTCGGGCTGGGCGGGATGCT
CTGCTTCCCGCTGCGCATTGGCGCCAGTACCGCGCTGTCGGAAAGGCTTACGCCGGAACTACTGCTCAAGCTGATCCAGG
ACCATCGCGCGACGATCGTTTTCACCGCGCCGACGTTCTATCGGCAGATGGCGGCGCTGGTAAAGAACTTCGATTTGTCG
AGCCTGAAGAAGAGCGTGTCTGCTGGCGAGGCGCTGCCCGATGCCACGCGGCAGGCCTGGAAGGCAGCCACTGGCATCGA
GATGACCGACGGCCTCGGCGGCACGGAGATGATGCATATCTTCGTTTCCAGTGCTGGAGCCGACGTGCGCCCGGGCGCAG
TGGGTCGCGTGGTACCGGGGTACGAAGCCCGTATCGTCGACGAGAACATGCAGCCGGTGCCGCCGGGCACGGTAGGCAAG
CTCGCGGTGCGCGGGCCCACGGGCTGCCGCTATCTGGACGATCCGCGCCAGGAGGCATATGTGCGCGACGGGTGGAATCT
GCCCGGCGACACATTCATGGCCGACGCCGATGGCTACTATTTCTATCAGGCGCGCTCGGACGACATGATCATCTCGGCCG
GGTACAACATCGCCGGGCCCGAGGTCGAGGGATCGCTGATGCGGCATGAGTGGGTGGCTGAATGCGGGGTGGTCGGCGCG
CCGGACGCGGAGCGTGGCCAGGTCGTGACGGCGTACGTGGTCCTGCGTCCCGGCGTGGAGCCCTCCGACGCCACGCGGGC
GGCGCTACAAGACTATGTCAAGCGCGAGATCGCCCCGTACAAGTATCCGCGTCGCATCGAGTTCGTGGCGGCGCTGCCGC
GTACCGAGACGGGCAAGCTGCAGCGCTTCCGCCTGCGGCAGATGGCGGCAGAGGGCGCGTAG

Upstream 100 bases:

>100_bases
TCGCTCGCTGCGGATCTACGAAGGCGCCACTGAAGTGCAGAAGCTGATCATTGCGCGCGAGACGCTGGCAGCCCGATAGA
ACACGAGACAGGAGACAAGT

Downstream 100 bases:

>100_bases
CCATGAGCGACGTGTTCACCAATCAGGTGCGTGTGCGTTTCAAGCATTGCGATACCGCCGGCATCGTTTTCTACCCGCGC
TATTTCGAGATGCTGAACGA

Product: acid-CoA ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MAAPQPATAHVDTFARDRLPPPQTWPVFQFNDDTRYPDRLNAAVELVDRHVAAGNGGRVAVRHERAGRIETVTYAELAAL
VDRIAHVLVEDMGLVPGNRVLLRGPNNLMMAASWLATLKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDARLRDELDA
NRRADGEYFCPTLERVRYFHGEGADALETAMAGKPDTFTACDTAADDICLIAFTSGTTGQPKGTMHFHRDVLAMCDLFSR
HVLKPTPDDVFCGTPPIAFTFGLGGMLCFPLRIGASTALSERLTPELLLKLIQDHRATIVFTAPTFYRQMAALVKNFDLS
SLKKSVSAGEALPDATRQAWKAATGIEMTDGLGGTEMMHIFVSSAGADVRPGAVGRVVPGYEARIVDENMQPVPPGTVGK
LAVRGPTGCRYLDDPRQEAYVRDGWNLPGDTFMADADGYYFYQARSDDMIISAGYNIAGPEVEGSLMRHEWVAECGVVGA
PDAERGQVVTAYVVLRPGVEPSDATRAALQDYVKREIAPYKYPRRIEFVAALPRTETGKLQRFRLRQMAAEGA

Sequences:

>Translated_553_residues
MAAPQPATAHVDTFARDRLPPPQTWPVFQFNDDTRYPDRLNAAVELVDRHVAAGNGGRVAVRHERAGRIETVTYAELAAL
VDRIAHVLVEDMGLVPGNRVLLRGPNNLMMAASWLATLKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDARLRDELDA
NRRADGEYFCPTLERVRYFHGEGADALETAMAGKPDTFTACDTAADDICLIAFTSGTTGQPKGTMHFHRDVLAMCDLFSR
HVLKPTPDDVFCGTPPIAFTFGLGGMLCFPLRIGASTALSERLTPELLLKLIQDHRATIVFTAPTFYRQMAALVKNFDLS
SLKKSVSAGEALPDATRQAWKAATGIEMTDGLGGTEMMHIFVSSAGADVRPGAVGRVVPGYEARIVDENMQPVPPGTVGK
LAVRGPTGCRYLDDPRQEAYVRDGWNLPGDTFMADADGYYFYQARSDDMIISAGYNIAGPEVEGSLMRHEWVAECGVVGA
PDAERGQVVTAYVVLRPGVEPSDATRAALQDYVKREIAPYKYPRRIEFVAALPRTETGKLQRFRLRQMAAEGA
>Mature_552_residues
AAPQPATAHVDTFARDRLPPPQTWPVFQFNDDTRYPDRLNAAVELVDRHVAAGNGGRVAVRHERAGRIETVTYAELAALV
DRIAHVLVEDMGLVPGNRVLLRGPNNLMMAASWLATLKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDARLRDELDAN
RRADGEYFCPTLERVRYFHGEGADALETAMAGKPDTFTACDTAADDICLIAFTSGTTGQPKGTMHFHRDVLAMCDLFSRH
VLKPTPDDVFCGTPPIAFTFGLGGMLCFPLRIGASTALSERLTPELLLKLIQDHRATIVFTAPTFYRQMAALVKNFDLSS
LKKSVSAGEALPDATRQAWKAATGIEMTDGLGGTEMMHIFVSSAGADVRPGAVGRVVPGYEARIVDENMQPVPPGTVGKL
AVRGPTGCRYLDDPRQEAYVRDGWNLPGDTFMADADGYYFYQARSDDMIISAGYNIAGPEVEGSLMRHEWVAECGVVGAP
DAERGQVVTAYVVLRPGVEPSDATRAALQDYVKREIAPYKYPRRIEFVAALPRTETGKLQRFRLRQMAAEGA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=31.588785046729, Blast_Score=246, Evalue=5e-65,
Organism=Homo sapiens, GI38505220, Length=494, Percent_Identity=30.9716599190283, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI122937307, Length=498, Percent_Identity=30.3212851405622, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI58082049, Length=491, Percent_Identity=29.938900203666, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI157311624, Length=491, Percent_Identity=28.9205702647658, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI157311622, Length=491, Percent_Identity=28.9205702647658, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI115511026, Length=543, Percent_Identity=27.9926335174954, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=26.7441860465116, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=26.7441860465116, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI156151445, Length=522, Percent_Identity=27.7777777777778, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI13375727, Length=556, Percent_Identity=27.158273381295, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI28416953, Length=551, Percent_Identity=26.8602540834846, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI8923896, Length=573, Percent_Identity=24.956369982548, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI42544134, Length=364, Percent_Identity=26.6483516483516, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI253970489, Length=369, Percent_Identity=28.1842818428184, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=25.0608272506083, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=28.1021897810219, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788107, Length=551, Percent_Identity=27.7676950998185, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI145693145, Length=488, Percent_Identity=28.6885245901639, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=25.9405940594059, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1786810, Length=497, Percent_Identity=25.3521126760563, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789201, Length=354, Percent_Identity=26.8361581920904, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1786529, Length=381, Percent_Identity=29.9212598425197, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786801, Length=511, Percent_Identity=24.4618395303327, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788595, Length=507, Percent_Identity=26.0355029585799, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=25.2788104089219, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=518, Percent_Identity=27.2200772200772, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=479, Percent_Identity=26.3048016701461, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=507, Percent_Identity=24.65483234714, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=365, Percent_Identity=29.8630136986301, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=365, Percent_Identity=29.8630136986301, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=365, Percent_Identity=29.8630136986301, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=504, Percent_Identity=26.1904761904762, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=483, Percent_Identity=24.8447204968944, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=27.1739130434783, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=349, Percent_Identity=28.6532951289398, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71983001, Length=565, Percent_Identity=24.7787610619469, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=565, Percent_Identity=24.7787610619469, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=361, Percent_Identity=25.207756232687, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323182, Length=573, Percent_Identity=28.6212914485166, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319264, Length=587, Percent_Identity=27.2572402044293, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319699, Length=450, Percent_Identity=26.8888888888889, Blast_Score=123, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24581924, Length=487, Percent_Identity=28.3367556468172, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21355181, Length=482, Percent_Identity=23.6514522821577, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24667955, Length=569, Percent_Identity=25.1318101933216, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.8484848484848, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI62472339, Length=537, Percent_Identity=24.9534450651769, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24667959, Length=367, Percent_Identity=26.9754768392371, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355225, Length=557, Percent_Identity=22.8007181328546, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18859661, Length=364, Percent_Identity=26.0989010989011, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648260, Length=496, Percent_Identity=25.2016129032258, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=23.3663366336634, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356441, Length=349, Percent_Identity=26.3610315186246, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653035, Length=495, Percent_Identity=23.6363636363636, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648257, Length=343, Percent_Identity=26.2390670553936, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20130357, Length=353, Percent_Identity=23.7960339943343, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 60308; Mature: 60176

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPQPATAHVDTFARDRLPPPQTWPVFQFNDDTRYPDRLNAAVELVDRHVAAGNGGRVA
CCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
VRHERAGRIETVTYAELAALVDRIAHVLVEDMGLVPGNRVLLRGPNNLMMAASWLATLKA
EEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHC
GLIAVPTMPLLRAKELKQIIDKAQVTAALCDARLRDELDANRRADGEYFCPTLERVRYFH
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHC
GEGADALETAMAGKPDTFTACDTAADDICLIAFTSGTTGQPKGTMHFHRDVLAMCDLFSR
CCCHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
HVLKPTPDDVFCGTPPIAFTFGLGGMLCFPLRIGASTALSERLTPELLLKLIQDHRATIV
HCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHCCHHHHHHCCCHHHHHHHHHCCCEEEE
FTAPTFYRQMAALVKNFDLSSLKKSVSAGEALPDATRQAWKAATGIEMTDGLGGTEMMHI
EECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHEEEE
FVSSAGADVRPGAVGRVVPGYEARIVDENMQPVPPGTVGKLAVRGPTGCRYLDDPRQEAY
EEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
VRDGWNLPGDTFMADADGYYFYQARSDDMIISAGYNIAGPEVEGSLMRHEWVAECGVVGA
HHCCCCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
PDAERGQVVTAYVVLRPGVEPSDATRAALQDYVKREIAPYKYPRRIEFVAALPRTETGKL
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHH
QRFRLRQMAAEGA
HHHHHHHHHHCCC
>Mature Secondary Structure 
AAPQPATAHVDTFARDRLPPPQTWPVFQFNDDTRYPDRLNAAVELVDRHVAAGNGGRVA
CCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
VRHERAGRIETVTYAELAALVDRIAHVLVEDMGLVPGNRVLLRGPNNLMMAASWLATLKA
EEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHC
GLIAVPTMPLLRAKELKQIIDKAQVTAALCDARLRDELDANRRADGEYFCPTLERVRYFH
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHC
GEGADALETAMAGKPDTFTACDTAADDICLIAFTSGTTGQPKGTMHFHRDVLAMCDLFSR
CCCHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
HVLKPTPDDVFCGTPPIAFTFGLGGMLCFPLRIGASTALSERLTPELLLKLIQDHRATIV
HCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHCCHHHHHHCCCHHHHHHHHHCCCEEEE
FTAPTFYRQMAALVKNFDLSSLKKSVSAGEALPDATRQAWKAATGIEMTDGLGGTEMMHI
EECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHEEEE
FVSSAGADVRPGAVGRVVPGYEARIVDENMQPVPPGTVGKLAVRGPTGCRYLDDPRQEAY
EEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
VRDGWNLPGDTFMADADGYYFYQARSDDMIISAGYNIAGPEVEGSLMRHEWVAECGVVGA
HHCCCCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
PDAERGQVVTAYVVLRPGVEPSDATRAALQDYVKREIAPYKYPRRIEFVAALPRTETGKL
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHH
QRFRLRQMAAEGA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]