Click here to switch to the map view.

The map label for this gene is ycdT [H]

Identifier: 94311111

GI number: 94311111

Start: 2378181

End: 2379431

Strand: Reverse

Name: ycdT [H]

Synonym: Rmet_2173

Alternate gene names: 94311111

Gene position: 2379431-2378181 (Counterclockwise)

Preceding gene: 335055584

Following gene: 94311110

Centisome position: 60.57

GC content: 65.39

Gene sequence:

>1251_bases
ATGTTTCAGTCGCAGGTGGTGGTGCTGATCGCAGGCTTGATAGCGCTGCAGATGGCGATCGTCTGTGCCGTGTTGCGGCG
CTCTGCGGGTATGGAGCGTAGCGGGCTCACGTCGTGGGCGCTCGGCGATCTGTTGGTGACCCTGGCCGTGGTCCTTCAGG
CCGCGCACGCATACCGGCCAGAGTGGCCGCTGGCGGTGCTCCCTGAGTTCGCCCTGACCGCGGGACTGTCGATGATGGTC
TACGGCACACGGCATTTCGCGGGCAAGCGTGGTCGTGGCGTGGTGCTTGGTGTGCTGAACCTCCCCGGGTTGGCCGCGCT
GACGATTTATGCGGTCGGCGGGCTTGCCGAAATGGGCCTGGGGCGTGTGCCGACCGGCACACTCGCCTGGCTCGGGCTCG
CCACGGCGTCGATCCAGACCCTGCTGCTACTGGAAATTGCCCGGATCACGGTTGCCCTGGTGCCGCTCAAGCGCGGCACG
GGTCGGCTGGCAGCACTCTCGTTATCTGTCCTGTCCCTTGGTTGCGCAGTACTGGCTGGCGCGGAGGCCTGGCCGATGTT
CGCCGCGCTCATGGACGGCCACGTGCAGCCGCTGGTTGATCGTCACGAAAGCATGACTGTCTGGTTCGTGTTCGGCGTGA
TGGGGCTGGCCGTCAGCTTCGCGCTGATGGCCCATGACCGTCTGCGCCAGATCATCGAACGTCGCGCGCGGCACGACGAC
CTAACCGATGTGCTATCTCGTGGCGCATTCTGGGAAGAACTCGAACTTGCCTGCAAGCAGGCGGAGCGCTCGCGCAAGCC
ACTTACCGTGGCGTTCATGGACCTGGACCACTTCAAGGCCATCAACGATCTCTATGGACACCTGGCTGGAGACAGCGTGC
TGCGCCATTTCGCGGGACTGCTGCGCAAGGTGGCGGCGCCGGACTCGGTGATCGGCCGTCTCGGCGGCGAGGAATTCGCG
ATCGTGATGCCTGCCACCGGACTGGAGACGGGCCGCGCCATCAGCGTCCGACTCAGCGCACTGGTGCGGGCGACACCGTG
CCCGTCCGAGCCAGACCCAATCGCCTACACGGTCAGCATCGGGATGGCGGAATGGCAGTTGGGCGAAAACGCCGACGCGG
TCATGCGTCGTTCGGATCGCGCGCTCTATGACGCCAAACAGATGGGCCGGAACTGCGTTTCCTCGCACGATGCGGAGGAG
TTCACCGGAATGCCGCGTCGCCCACGCGTGCGCGAACGCGAACCTTCGTAA

Upstream 100 bases:

>100_bases
ACCTGCGGAAAACAAAATATGACGGCTTGTCGCGGGCCGGAATCAGAAGGTGCGCCATTGCCGTGCGGAGCATGACGACA
CCTTTGGAACGGGAATTGAG

Downstream 100 bases:

>100_bases
AGCAGACGATGTGTCCTGTGCGCGGCATTCGTGCTGCGGTAGAATGCACGCCTTGCCGCAATGGCAAGGCCGCGCCGCCC
TTAGCTCAGTTGGATAGAGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRPEWPLAVLPEFALTAGLSMMV
YGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGLGRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGT
GRLAALSLSVLSLGCAVLAGAEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDD
LTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGLLRKVAAPDSVIGRLGGEEFA
IVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSIGMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEE
FTGMPRRPRVREREPS

Sequences:

>Translated_416_residues
MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRPEWPLAVLPEFALTAGLSMMV
YGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGLGRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGT
GRLAALSLSVLSLGCAVLAGAEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDD
LTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGLLRKVAAPDSVIGRLGGEEFA
IVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSIGMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEE
FTGMPRRPRVREREPS
>Mature_416_residues
MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRPEWPLAVLPEFALTAGLSMMV
YGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGLGRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGT
GRLAALSLSVLSLGCAVLAGAEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDD
LTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGLLRKVAAPDSVIGRLGGEEFA
IVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSIGMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEE
FTGMPRRPRVREREPS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=211, Percent_Identity=33.175355450237, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=32.972972972973, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=36.4161849710983, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=34.8314606741573, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=31.8181818181818, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=36.4779874213836, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786584, Length=185, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=33.5260115606936, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=29.9435028248588, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=234, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=31.2101910828025, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44922; Mature: 44922

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EWPLAVLPEFALTAGLSMMVYGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGL
CCCHHHHHHHHHHHCCHHEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
GRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGTGRLAALSLSVLSLGCAVLAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCC
AEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDD
CCHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGL
HHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKVAAPDSVIGRLGGEEFAIVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSI
HHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEE
GMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEEFTGMPRRPRVREREPS
CCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EWPLAVLPEFALTAGLSMMVYGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGL
CCCHHHHHHHHHHHCCHHEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
GRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGTGRLAALSLSVLSLGCAVLAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCC
AEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDD
CCHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGL
HHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKVAAPDSVIGRLGGEEFAIVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSI
HHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEE
GMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEEFTGMPRRPRVREREPS
CCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]