Click here to switch to the map view.

The map label for this gene is mrcA1 [H]

Identifier: 94311090

GI number: 94311090

Start: 2348481

End: 2350802

Strand: Direct

Name: mrcA1 [H]

Synonym: Rmet_2152

Alternate gene names: 94311090

Gene position: 2348481-2350802 (Clockwise)

Preceding gene: 94311089

Following gene: 94311091

Centisome position: 59.79

GC content: 64.17

Gene sequence:

>2322_bases
ATGCCAACAGAGTCAAAGCCGGAAACCACCAACACTCCCGGACGGGCCCGGATTGCAACCTGCCTGGGCGCGCTTGCCGG
TCTCCTTATCGCGCTGGGAATGGCCTTTGGCGCCTGGCACGTCTATCAGGCATGGCTGTCGCTCCCGGACGTCAATAGCC
TTGCCGCGTTCAAGCCAGACAGGCCGCTGCGCATTTATTCTCAGGATGGCATCCTCCTGGCCGAGTATGGCGACGAGCGC
CGGGAGATCGTCCCGCTTTCCAGAATTCCGATCGTCGTGCAGCAGAGCCTGCTGGCGATCGAAGATGCGCGGTTCTACGA
ACACGGTGGCGTGGACTTCTCGGGATTGTTCCGCGCTGCATGGGCCAACTTCGCATCGGGCCGGCACGCGCAGGGGGCCA
GCACCATCACCATGCAGGTAGCGCGCCGCCTGTACCTGTCCAGCGAGAAGACTTACGACCGGAAGCTGCGCGAAGTTCTT
CTGGCCTTCAAGCTGGAGCAGCAGTTCAGCAAGCAACGGCTGCTTGAGGTCTATATGAACCAGATCTATCTGGGCGAGCG
CGCATACGGATTTGCTGCCGCGGCGAGCATCTATTTCGACAAGTCACTCGACCAGTTGACCCCAGCGGAAGCCGCCCTGC
TCGCGGGCCTCCCCAAAGCACCGTCCGCATTCAACCCGACGGTCAACCCGGAGCGTGCCCGCGTACGCCAGCGGTACATC
CTCGCGCGCATGCACGAGCTTGGATATCTGGGCGACGAACAGTACGCATCCGCATTGGATGAACCGCTTCGGCTGAAGCC
ACGTAGCTATGCCATCGACCCGGCATTTGCCTTTCCGGTGGAGCGCGCACGCCAACTGGTCGTGGACCAGTACGGTGAAC
GCGCCTATCGCGATGGCCTGGACGTGACGCTAACCATTCAAACCGCCACGCAGGCAGCCGCTTACGATGCCCTGCATGAC
GGCCTGCTTCGCGCCCAGCAAAGACGACGCTATATCCCGCTGGGACGGGTGGCCATCGACGGCAATGCGCCAGCATCGGG
GCTTGACGACTATCCGGATCGGGACGGCCTCACCACGGCCGTCGTCACCGCCACGGCCGATCGCAGCGGCCGCCTGACGG
TCAAGACGCGCGATGGCACGACCCTTCGTGCGCAAGCACCCGCGTATGCCGCGACGCCGCTGACGCGCGGCACGGTTGTC
ACCATGGCAAGCCTGGGTGGCAAATGGGAGATCGGGCAAGCGCCCGACATGGAGGGCGCGCTGGTTTCCATCGATTCCGT
CAGCGGCGATGTCGTGGCGTGGGTGGGTGGCTATGACTTCCATCGCGGCAAGTTTGATCACGCCGTGCAGGCGTTGCGCC
AGCCGGGCTCCTGCTTCAAACCCTTTGTCTACTCCGCCTCTCTCGAAAAAGGCTACTTTCCGGGAACCATGGTGGACGAC
AGTCAGCGGGTACTGGACTCGACCGTCACCGGTGCCCGGCCCTGGCGGCCCAAGAACTACGGCGGCCGCTACGACGGCTT
CATCACGGCACGCGAGGGGCTTGTCCGCTCCAAGAACCTGGTGACGGTGGGGCTGACGCAGGCTTCCGGTATCGATTACG
TGCGGGATTTCGCGCTGCGATTCGGCTTCGCGCCAGACCTGAATCCCGGCAGTTTCCCCCTGGCGCTGGGCGCCGGCAGC
GTGACGCCGATGCAATTGACGCAGGCGTACGCCGTCTTCTCCAACGGCGGCTATCTCGTGCAGCCGCGCCTGATCGCCCG
GATCGTGCAGCGCGGTGACCAGGAGCTCTACACATCGGCCGGAGACATCGAGCGCAAACAGATCATCTCCCCACGCAACG
CGTTCATCATGGACTCGCTGCTTCGCGATGTGGTTCGGAAAGGCACCGGTCGCGCGGCGGCCCATATCGACAAGGCCGAC
GCTGCCGGCAAGACGGGGACCTCGAACGATGCCAGGGACGCATGGTTCGCCGGATACGCGACGGGGCTGGCGACCACCGT
ATGGGTAGGCCATGACCAGTTCCGCAGCCTCGGGGCGGTCACGGGTGGGACGCTTGCACTGCCGATCTGGACGGACTTCA
TGCGCCACGCCACGGCTGACCGCGCCCCGATCAGCAGAGCGTTGCCGGACGATGTTGTCGAGGAAAACGGCGACTACATC
TACCGCGAGTATCGTTCGTCCGCCTGCGTGAGCGATTCCCACGCATTCATCCGGACGGGATACCGCTGCGCATCCGAGCC
CGCGCCTGTCCCGACACGCGAGCAGCAACGCGAGCACGAGCAGATTCTGGAGTGGTTCCGGCAAGACGCCGATAACGTGT
GA

Upstream 100 bases:

>100_bases
GTCTTGTATGCAGTCCGGAAACCGTCCGACAGACTGATGGTCACAGCCACCCTCAGGATATACCGGCGCGCGGCGATCCA
CGCGTGCCCAGCCTTCAGCG

Downstream 100 bases:

>100_bases
CGCCTCGGGCGCGATTGCTGCAAGGCTTATAATTCAGCGCCTGCGATACGTCTTGGAAACCGCAAAATGCCCATTTCGAT
CATGGTCCTGGATGACCACG

Product: transpeptidase/transglycosylase (peniciline binding)

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MPTESKPETTNTPGRARIATCLGALAGLLIALGMAFGAWHVYQAWLSLPDVNSLAAFKPDRPLRIYSQDGILLAEYGDER
REIVPLSRIPIVVQQSLLAIEDARFYEHGGVDFSGLFRAAWANFASGRHAQGASTITMQVARRLYLSSEKTYDRKLREVL
LAFKLEQQFSKQRLLEVYMNQIYLGERAYGFAAAASIYFDKSLDQLTPAEAALLAGLPKAPSAFNPTVNPERARVRQRYI
LARMHELGYLGDEQYASALDEPLRLKPRSYAIDPAFAFPVERARQLVVDQYGERAYRDGLDVTLTIQTATQAAAYDALHD
GLLRAQQRRRYIPLGRVAIDGNAPASGLDDYPDRDGLTTAVVTATADRSGRLTVKTRDGTTLRAQAPAYAATPLTRGTVV
TMASLGGKWEIGQAPDMEGALVSIDSVSGDVVAWVGGYDFHRGKFDHAVQALRQPGSCFKPFVYSASLEKGYFPGTMVDD
SQRVLDSTVTGARPWRPKNYGGRYDGFITAREGLVRSKNLVTVGLTQASGIDYVRDFALRFGFAPDLNPGSFPLALGAGS
VTPMQLTQAYAVFSNGGYLVQPRLIARIVQRGDQELYTSAGDIERKQIISPRNAFIMDSLLRDVVRKGTGRAAAHIDKAD
AAGKTGTSNDARDAWFAGYATGLATTVWVGHDQFRSLGAVTGGTLALPIWTDFMRHATADRAPISRALPDDVVEENGDYI
YREYRSSACVSDSHAFIRTGYRCASEPAPVPTREQQREHEQILEWFRQDADNV

Sequences:

>Translated_773_residues
MPTESKPETTNTPGRARIATCLGALAGLLIALGMAFGAWHVYQAWLSLPDVNSLAAFKPDRPLRIYSQDGILLAEYGDER
REIVPLSRIPIVVQQSLLAIEDARFYEHGGVDFSGLFRAAWANFASGRHAQGASTITMQVARRLYLSSEKTYDRKLREVL
LAFKLEQQFSKQRLLEVYMNQIYLGERAYGFAAAASIYFDKSLDQLTPAEAALLAGLPKAPSAFNPTVNPERARVRQRYI
LARMHELGYLGDEQYASALDEPLRLKPRSYAIDPAFAFPVERARQLVVDQYGERAYRDGLDVTLTIQTATQAAAYDALHD
GLLRAQQRRRYIPLGRVAIDGNAPASGLDDYPDRDGLTTAVVTATADRSGRLTVKTRDGTTLRAQAPAYAATPLTRGTVV
TMASLGGKWEIGQAPDMEGALVSIDSVSGDVVAWVGGYDFHRGKFDHAVQALRQPGSCFKPFVYSASLEKGYFPGTMVDD
SQRVLDSTVTGARPWRPKNYGGRYDGFITAREGLVRSKNLVTVGLTQASGIDYVRDFALRFGFAPDLNPGSFPLALGAGS
VTPMQLTQAYAVFSNGGYLVQPRLIARIVQRGDQELYTSAGDIERKQIISPRNAFIMDSLLRDVVRKGTGRAAAHIDKAD
AAGKTGTSNDARDAWFAGYATGLATTVWVGHDQFRSLGAVTGGTLALPIWTDFMRHATADRAPISRALPDDVVEENGDYI
YREYRSSACVSDSHAFIRTGYRCASEPAPVPTREQQREHEQILEWFRQDADNV
>Mature_772_residues
PTESKPETTNTPGRARIATCLGALAGLLIALGMAFGAWHVYQAWLSLPDVNSLAAFKPDRPLRIYSQDGILLAEYGDERR
EIVPLSRIPIVVQQSLLAIEDARFYEHGGVDFSGLFRAAWANFASGRHAQGASTITMQVARRLYLSSEKTYDRKLREVLL
AFKLEQQFSKQRLLEVYMNQIYLGERAYGFAAAASIYFDKSLDQLTPAEAALLAGLPKAPSAFNPTVNPERARVRQRYIL
ARMHELGYLGDEQYASALDEPLRLKPRSYAIDPAFAFPVERARQLVVDQYGERAYRDGLDVTLTIQTATQAAAYDALHDG
LLRAQQRRRYIPLGRVAIDGNAPASGLDDYPDRDGLTTAVVTATADRSGRLTVKTRDGTTLRAQAPAYAATPLTRGTVVT
MASLGGKWEIGQAPDMEGALVSIDSVSGDVVAWVGGYDFHRGKFDHAVQALRQPGSCFKPFVYSASLEKGYFPGTMVDDS
QRVLDSTVTGARPWRPKNYGGRYDGFITAREGLVRSKNLVTVGLTQASGIDYVRDFALRFGFAPDLNPGSFPLALGAGSV
TPMQLTQAYAVFSNGGYLVQPRLIARIVQRGDQELYTSAGDIERKQIISPRNAFIMDSLLRDVVRKGTGRAAAHIDKADA
AGKTGTSNDARDAWFAGYATGLATTVWVGHDQFRSLGAVTGGTLALPIWTDFMRHATADRAPISRALPDDVVEENGDYIY
REYRSSACVSDSHAFIRTGYRCASEPAPVPTREQQREHEQILEWFRQDADNV

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=592, Percent_Identity=38.8513513513513, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1786343, Length=264, Percent_Identity=34.0909090909091, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788867, Length=272, Percent_Identity=34.5588235294118, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=35.7575757575758, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 84941; Mature: 84810

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTESKPETTNTPGRARIATCLGALAGLLIALGMAFGAWHVYQAWLSLPDVNSLAAFKPD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
RPLRIYSQDGILLAEYGDERREIVPLSRIPIVVQQSLLAIEDARFYEHGGVDFSGLFRAA
CCEEEECCCCEEEECCCCCCCEECCHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
WANFASGRHAQGASTITMQVARRLYLSSEKTYDRKLREVLLAFKLEQQFSKQRLLEVYMN
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIYLGERAYGFAAAASIYFDKSLDQLTPAEAALLAGLPKAPSAFNPTVNPERARVRQRYI
HHHCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LARMHELGYLGDEQYASALDEPLRLKPRSYAIDPAFAFPVERARQLVVDQYGERAYRDGL
HHHHHHHCCCCCHHHHHHCCCCCEECCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHCCC
DVTLTIQTATQAAAYDALHDGLLRAQQRRRYIPLGRVAIDGNAPASGLDDYPDRDGLTTA
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
VVTATADRSGRLTVKTRDGTTLRAQAPAYAATPLTRGTVVTMASLGGKWEIGQAPDMEGA
EEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEHHHCCCEECCCCCCCCCCC
LVSIDSVSGDVVAWVGGYDFHRGKFDHAVQALRQPGSCFKPFVYSASLEKGYFPGTMVDD
EEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCC
SQRVLDSTVTGARPWRPKNYGGRYDGFITAREGLVRSKNLVTVGLTQASGIDYVRDFALR
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCHHCCCCEEEEEECCCCCHHHHHHHHHH
FGFAPDLNPGSFPLALGAGSVTPMQLTQAYAVFSNGGYLVQPRLIARIVQRGDQELYTSA
HCCCCCCCCCCCEEEECCCCCCHHHHHHHHHEEECCCEEECHHHHHHHHHCCCHHHHHCC
GDIERKQIISPRNAFIMDSLLRDVVRKGTGRAAAHIDKADAAGKTGTSNDARDAWFAGYA
CCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHH
TGLATTVWVGHDQFRSLGAVTGGTLALPIWTDFMRHATADRAPISRALPDDVVEENGDYI
HHHHHEEEECCHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCHHCCCHHHHHCCCCEE
YREYRSSACVSDSHAFIRTGYRCASEPAPVPTREQQREHEQILEWFRQDADNV
HHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTESKPETTNTPGRARIATCLGALAGLLIALGMAFGAWHVYQAWLSLPDVNSLAAFKPD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
RPLRIYSQDGILLAEYGDERREIVPLSRIPIVVQQSLLAIEDARFYEHGGVDFSGLFRAA
CCEEEECCCCEEEECCCCCCCEECCHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
WANFASGRHAQGASTITMQVARRLYLSSEKTYDRKLREVLLAFKLEQQFSKQRLLEVYMN
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIYLGERAYGFAAAASIYFDKSLDQLTPAEAALLAGLPKAPSAFNPTVNPERARVRQRYI
HHHCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LARMHELGYLGDEQYASALDEPLRLKPRSYAIDPAFAFPVERARQLVVDQYGERAYRDGL
HHHHHHHCCCCCHHHHHHCCCCCEECCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHCCC
DVTLTIQTATQAAAYDALHDGLLRAQQRRRYIPLGRVAIDGNAPASGLDDYPDRDGLTTA
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
VVTATADRSGRLTVKTRDGTTLRAQAPAYAATPLTRGTVVTMASLGGKWEIGQAPDMEGA
EEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEHHHCCCEECCCCCCCCCCC
LVSIDSVSGDVVAWVGGYDFHRGKFDHAVQALRQPGSCFKPFVYSASLEKGYFPGTMVDD
EEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCC
SQRVLDSTVTGARPWRPKNYGGRYDGFITAREGLVRSKNLVTVGLTQASGIDYVRDFALR
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCHHCCCCEEEEEECCCCCHHHHHHHHHH
FGFAPDLNPGSFPLALGAGSVTPMQLTQAYAVFSNGGYLVQPRLIARIVQRGDQELYTSA
HCCCCCCCCCCCEEEECCCCCCHHHHHHHHHEEECCCEEECHHHHHHHHHCCCHHHHHCC
GDIERKQIISPRNAFIMDSLLRDVVRKGTGRAAAHIDKADAAGKTGTSNDARDAWFAGYA
CCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCCHHHHHHHH
TGLATTVWVGHDQFRSLGAVTGGTLALPIWTDFMRHATADRAPISRALPDDVVEENGDYI
HHHHHEEEECCHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCHHCCCHHHHHCCCCEE
YREYRSSACVSDSHAFIRTGYRCASEPAPVPTREQQREHEQILEWFRQDADNV
HHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA