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The map label for this gene is rpoS [H]

Identifier: 94311053

GI number: 94311053

Start: 2296755

End: 2297915

Strand: Reverse

Name: rpoS [H]

Synonym: Rmet_2115

Alternate gene names: 94311053

Gene position: 2297915-2296755 (Counterclockwise)

Preceding gene: 94311054

Following gene: 94311052

Centisome position: 58.5

GC content: 61.58

Gene sequence:

>1161_bases
ATGCCACGCCAGAAAACTGTCTCATCCGGAGTTGTCAGCAGGACCCGTCGTCCCAAGCAGCCGGAAGGAAAGGCTGGAGT
GGCAAGACCTGACGGCGGGATCGACACGGAAATGTTCGGCGACGATCCGGCCGCCGAACTGGTACCCGTCGCCGACGAAC
CGATCGCGCCAGTTTCGCGCGTCGGCCTGCGCAACACGGACTTGAATGCGGACGACGCCGATCACGATGACGGCGACGAG
GAAGAATCCGAAGAGGATGAGGAAGAGGGCAGCAGTGAGACCACTGCCGAGCCCGATGACTTCCGTACCGTGTTGCACAC
GGAACTCGCCGCCGACACGGTCCAGCATTATCTCAATCGCATCAGCATCAAGCCGCTGCTCTCCGCGCCGGAGGAACTCC
ATTTCTCCACGCTGGCCAAGGGCGGTGACTTTTCCGCGCGTCAGGTGATGATCGAGCGCAATCTGCGTCTCGTGGTCAGC
ATCGCCAAGGGCTATCTGAATCGGGGCGTGCCGCTGCTTGACTTGATCGAGGAAGGCAACCTGGGCCTGATGCACGCGAT
CGAGAAATTCGATCCTTCGCGCGGATTCCGCTTTTCGACGTATGCGACCTGGTGGATTCGCCAGAGCATCGAGCGCGCGA
TCATGAACCAGGCACGCACCGTGCGCTTGCCTGTGCACGTGATTCGCGAACTCAACCAGGTACTGCGCGCCAAGCGCCAT
CTTGAAAAAGGTGGCACCGATGGCCGCGACGCCAGCCTCGAGGACATCGCTCACCTGCTCGGCAAGACGCCGGACGAGGT
GCAGGACGTGCTGGCACTCAATGAACATACCACCTCGCTCGACACGCCGTTCGACCTCGACCCGGGCTCCAGCCTGCTCG
ATTTCCTTTCCGACGAGCATAACGCCGCACCCGATCAGGAAGTGGCCCACCGCGAGCTGGAAAACCTGATGAAACTCTGG
CTGGCGCGCCTGTCGGAGAAGCATCGCTATGTGGTGGAGCGCCGTTTCGGCCTCAATCACATCGAGCCGGCCACGCTCGA
GGAGCTGGCCGAGGAAATGGGTCTCACGCGCGAGCGCGTGCGTCAGATCCAGCAGGAAGCGCTGGTCAAGCTCAAACGGC
ATTTCGCTTCGCAAGGTGTACGCAAGGACGCCGTTCTATGA

Upstream 100 bases:

>100_bases
GGGTAACAGTGATTCTGACAGGGTCAAGCTGCACTTCGAGGTCCGCAAAAACGGGAAACCGGTCGATCCGATGCGGTATC
TGCCGCCACAGTGAGGTTTC

Downstream 100 bases:

>100_bases
CCCCTGTGCTGGTATTCGACATCGAGACAATTCCCGATGTCGACGGCCTGCGCCGTTTGCATGACCATCCCGCATCGATG
AGCGACGCCGAAGTCGCCGA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MPRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSRVGLRNTDLNADDADHDDGDE
EESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVS
IAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRH
LEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELENLMKLW
LARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Sequences:

>Translated_386_residues
MPRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSRVGLRNTDLNADDADHDDGDE
EESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVS
IAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRH
LEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELENLMKLW
LARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL
>Mature_385_residues
PRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSRVGLRNTDLNADDADHDDGDEE
ESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVSI
AKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHL
EKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELENLMKLWL
ARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=332, Percent_Identity=48.1927710843374, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=41.9491525423729, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=31.1355311355311, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43311; Mature: 43180

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCEECCCCCCCCHHHH
VGLRNTDLNADDADHDDGDEEESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGN
CCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCC
LGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRH
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHH
LEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEH
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCC
NAAPDQEVAHRELENLMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHH
RQIQQEALVKLKRHFASQGVRKDAVL
HHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCEECCCCCCCCHHHH
VGLRNTDLNADDADHDDGDEEESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGN
CCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCC
LGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRH
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHH
LEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEH
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCC
NAAPDQEVAHRELENLMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHH
RQIQQEALVKLKRHFASQGVRKDAVL
HHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]