Click here to switch to the map view.

The map label for this gene is rumA [H]

Identifier: 94311050

GI number: 94311050

Start: 2293575

End: 2295050

Strand: Reverse

Name: rumA [H]

Synonym: Rmet_2112

Alternate gene names: 94311050

Gene position: 2295050-2293575 (Counterclockwise)

Preceding gene: 94311052

Following gene: 94311048

Centisome position: 58.43

GC content: 64.36

Gene sequence:

>1476_bases
ATGTCGGGCGCCGCGACTGGACTACAATCGCGCTTCTGCAAAACAATACGACAGGTCCCACTGGTGTCTCAAGCCGTGTC
TTCCCAGCCCAATCCGACAAGCCACCCGGAAGCGGCATCTGCCGCTTCCGCCGCTTCCAATGATCCCGTCGTCAAGATCG
ACAGCCTCGACATGGAGGCGCGTGGCGTCGGCCGTCTGGAGAACGAGGACGGCACGCCAGGCAAGGTGATTTTCGTCGAG
GGCGCGCTGCCCGGCGAGACGGTTGCGTATCGCAGCTATCGCCGCAAGCCTAGCTACGAACAGGCCCATCTCGTCGAGGT
GAAGCAGGCCTCCGTCATGCGGGTGAAGCCGGGTTGCCAGCATTTTGGCGTGTGCGGCGGCTGCTCGATGCAGCACCTGG
ACTCGCGCGCGCAACTGGCAATCAAGCAGCGTGTGCTCGAAGACAATCTCTGGCACCTGTCGAAGGTGAAGCCCGACGTC
GTGTTCCGCCCGATCGCGGGCCCGGATTGGGGCTATCGCTACCGCGCCCGTCTGACGGTGCGGCACGTTGTCAAGAAGGG
TGGAGTACTGGTGGGCTTCCACGAGCGCAAGAGCAGCTATGTGGCGGACATGACGCACTGCGAGATCCTGCCGCCGCATG
TGTCGGCAATGCTGGTGCCGCTGCGCGAATTGGTGGGCGGCCTGTCGATCCATGATCGGATGCCGCAGATCGAACTGGCC
GTTGGTGCGGAGGTGACCGCACTTGTGTTGCGCGTCCTGGAGCCGCTCAACGACGCCGACAAGGACTTGCTGCGGGCCTT
CGCCGATCAGCATCAGGTGCAGTTCTGGCTCCAGCCGAAGGGGCCGGACACGGTCTATCCGTTTTATCCGGCCGAGCAGG
AGCTGGCCTATACACTGCCCGAGTTCGGCATCCGGATGCCGTTCAAGCCGACCGACTTCACGCAGGTCAATCACCAGATC
AATCGCGTGCTGATTGGTCGCGCGCTACGTCTGCTGGATGCGAAGCCGACTGACCGCCTGCTCGACCTGTTCTGCGGCAT
CGGCAATTTCACGCTGCCGCTGGCGACGCAGGGTGCATCTGTCATGGGGATCGAGGGAAGCGAGGTGCTGACCACGCGTG
CGCTGGCCAATGCCGAGTACAACGGGCTGGCAGCCAAGACGTCCTTCGCCTGCCGCAATCTGTTCGAGGTGACCGCCGAG
GACATCGCAGCGCTGGGCCGCTTCGACCGCTGGCTGGTAGACCCGCCGCGCGAGGGTGCGCTGGCTGTCTGCAAGGCGCT
TGCTGAACTGAGTCAGGCCGGTAGCGACGTGCTGCCGAAGCGTATCGTCTATGTGTCGTGCAGTCCGGCGACGCTGGCGC
GTGACGCGGGCTTGCTGGTGCACGAGGCCGGCTACCGCCTGGCCGGCGCGGGCGTGGTCAATATGTTCCCGCATACGTCG
CACGTGGAGTCGATCGCGGTGTTCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
CACGCAGGGTGTCGACGATGCGGGCGAGATCGACCTTGCCGTCGGCCCCGCGGCCCCACTGGATATTCCACGAAATCAGT
TCCATAGCCTGCCCCCTTCG

Downstream 100 bases:

>100_bases
ATCCACATGGCGCACCCATGAAAAAAACCGCCCGAGGGCGGCTTTTTTTCGTTGGCAGATGCAGGTCAGTCGCGGCTGCC
GCCTGCGATGCCGAGCAGGG

Product: 23S rRNA m(5)U1939 methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSGAATGLQSRFCKTIRQVPLVSQAVSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEARGVGRLENEDGTPGKVIFVE
GALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDV
VFRPIAGPDWGYRYRARLTVRHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELA
VGAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLPEFGIRMPFKPTDFTQVNHQI
NRVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGASVMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAE
DIAALGRFDRWLVDPPREGALAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTS
HVESIAVFERD

Sequences:

>Translated_491_residues
MSGAATGLQSRFCKTIRQVPLVSQAVSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEARGVGRLENEDGTPGKVIFVE
GALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDV
VFRPIAGPDWGYRYRARLTVRHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELA
VGAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLPEFGIRMPFKPTDFTQVNHQI
NRVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGASVMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAE
DIAALGRFDRWLVDPPREGALAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTS
HVESIAVFERD
>Mature_490_residues
SGAATGLQSRFCKTIRQVPLVSQAVSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEARGVGRLENEDGTPGKVIFVEG
ALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDVV
FRPIAGPDWGYRYRARLTVRHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELAV
GAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLPEFGIRMPFKPTDFTQVNHQIN
RVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGASVMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAED
IAALGRFDRWLVDPPREGALAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSH
VESIAVFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=38.3408071748879, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1787083, Length=348, Percent_Identity=24.4252873563218, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 53658; Mature: 53527

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAATGLQSRFCKTIRQVPLVSQAVSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEA
CCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCC
RGVGRLENEDGTPGKVIFVEGALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQ
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCCCC
HFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDVVFRPIAGPDWGYRYRARLTV
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCCCEEEEHHHH
RHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELA
HHHHHHCCEEEEEHHCCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCHHCCCCCEEEE
VGAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLP
CCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCC
EFGIRMPFKPTDFTQVNHQINRVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGAS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCE
VMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAEDIAALGRFDRWLVDPPREGA
EEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTS
HHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCCEEEECCCCEEECCCEEECCCCCC
HVESIAVFERD
CCCEEEEEECC
>Mature Secondary Structure 
SGAATGLQSRFCKTIRQVPLVSQAVSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEA
CCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCC
RGVGRLENEDGTPGKVIFVEGALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQ
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCCCC
HFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDVVFRPIAGPDWGYRYRARLTV
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCCCEEEEHHHH
RHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELA
HHHHHHCCEEEEEHHCCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCHHCCCCCEEEE
VGAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLP
CCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCC
EFGIRMPFKPTDFTQVNHQINRVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGAS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCE
VMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAEDIAALGRFDRWLVDPPREGA
EEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTS
HHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCCEEEECCCCEEECCCEEECCCCCC
HVESIAVFERD
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA