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The map label for this gene is narX [H]

Identifier: 94311024

GI number: 94311024

Start: 2264669

End: 2266684

Strand: Direct

Name: narX [H]

Synonym: Rmet_2086

Alternate gene names: 94311024

Gene position: 2264669-2266684 (Clockwise)

Preceding gene: 94311021

Following gene: 94311025

Centisome position: 57.65

GC content: 64.09

Gene sequence:

>2016_bases
ATGCTAGCCCCTACGAATCCAGATACCACTGCCCCCCAGCCTGACAACGCCCACGTGCCGGAAGCGGTCCGGCTGACGCC
ACTGCGCCACCGGCTTTCCACCCGGATCATTCTGTTGTCGTTTGCGTCACTGATGGTGGTGCTGGGCATGATCTCAGGCA
CGCTGTATCTCTCCTGGAAGCTGGAAGGAGCCGGCGCCGCCATCAACGACGCCGGGAGCCTGCGGATGCGCGCGAACCGG
GTCGCCATTGAACTGACCCGTGCCCAAAGCGGCAGTCCCAACGATCTGGCAGGGCAAGTCGCGGCGCTGGACACCACGCT
CGCGCAGTTGCGCCGTGGCAATCCGGCCCGGCCGCTATTCTTGCCCGACGAACCCGCGATCCAGGCCAAGCTCGACCTCG
TCGTCACGGACTGGCAGCAGCGCCTGAAGCCGCTTGCGGTGGCCACCGCAAGCACCACGCCTGCAACGGCCTACATCGAT
GCCCTGCCAGTATTCGTCAATGAGGCCGACAGCCTGGTAAAACTGATCGAACGCGATAACGCGAGCAAAACCGATCTGCT
GCGACTCTCTCAGGCAAGCCTTGCGATCATCGCGTGCGTTGGCACGGTCGCTGTGATCTATCTGCTCTATCTCTGGATCA
TTCTGCCCGTGCTGCGTCTGCAGGATGGCCTGCAGCGCATGGCTGCCCGCGAGTTCTCGCTGCGCTTGCCCATCGCCACA
CGTGATGAATTCGGCACGCTGAACGCCGGCTTCAACCGCATGGCCGACGAACTTCAAGAGCTCTATCAGGACCTGGCGGC
ACGCGTGGACAGCAAGACTGCTGAGTTGGCGCGGCAGAATCGGGATTTGGAGGCCCTCTACGACATGACCGCCTTCCTGA
ATCGGGCCAGCGATGCCGAGGCGATGGCACGCGGATTCCTGGAGCGCGTGATGCGCCAGTTCGAAGCCGACGGCGGGACC
GTTCGTGTGATCGATGCCGCGCACGGAAAGCTCCACCGACTTGCCTCGGTGGGACTTTCGGACGCACTGGCGGAGAACAG
CCGCTGTAGCGCGATTCATGACTGCCACTGCGGGGCCGCAGCTGAAGCGGGCATGATCACTGTAGCGGACCTGCGCCAGT
TCGTGCGTCAGGGCTCCGAAACACAAGAATCTCCCTGTGGCCGCGAAGGTTTCAGGGGCATGGCGGCGTTCCGGCTCGAC
TCTCCACGCGGCACGCTCGGCACATTCACCCTGCATTTCCGCAATCCGCGCGAACTGCCACCTTCCGATCGTCAACTGCT
CCAAACCCTCGCCCGCCACCTTGGCACGGCGCTGGAGCACCTGCGGCTTGCCGCCAGCGCGCGCCAGCTTGCGGTCGTCG
AAGAGCGCAATCTCGTGGCGCAAGGATTGCACGACAGCATCGCGCAAGGCCTGAACTTCCTGAACCTTCAGGTACAACTG
CTCGGCGATGCGGTGGAACGGAACGACAATGCGGAAACGCGCGAGATCGTGCCGCTGCTGCGACACGGCGTGGAAGAAAG
CTACCAGGACGTGCGCGAACTGCTGCACAACTTCCGTTCCCGGCTGACGACGGGCGACATGCGCCCAGCGGTGGAGGACA
CCGTCGCGCGCTTCCGCCGCCAATGCCGTACGGACGCCAGGTTGACCATCGAAGGAAGCGGGCCGCCGCTGCAGCCGGAG
CAGCAGCTACAGGTGCTGTTCATCCTGCAGGAGGCGTTGTCGAACGTGCGCAAACATGCGATGGCCGAGCATGTCACGAT
CTCGCTCACGCAGGGGCGCGACTTCCAACTCGTCGTCCAGGACGATGGCGAAGGATTCGATCCTTCCGAATTCGACGGCC
AGGACAGCGCGCACGTCGGTCTGCACATCATGCAGGAACGGGCATCGCGGCTTGGCGCGACCCTGACGATCGACGCCCGC
CCAGGGGCTGGCGTCCGCGTCGCACTATCATTGCCGCGCGGCGAAACCATGCCGCATCCAGACGAGTTGCTGGCGGCGCA
GGAGTCTTCCCAATGA

Upstream 100 bases:

>100_bases
GCCACCATCACACGCTGGCACAATTTGCGGCAGGGCCGCCCGTCCCCACTCATACTGCAGTATCACCCTGCAATATCACC
CGCCGGGTCGCACCTTCCCA

Downstream 100 bases:

>100_bases
CCATCCGTATCCTACTGATCGACGATCACACATTGTTCCGCTCGGGTGTCCGTGCGCTGCTGCAGCGGCAGAGCGATTTC
GAAGTGGTCGACGAAGCCGC

Product: transmembrane nitrate/nitrite sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MLAPTNPDTTAPQPDNAHVPEAVRLTPLRHRLSTRIILLSFASLMVVLGMISGTLYLSWKLEGAGAAINDAGSLRMRANR
VAIELTRAQSGSPNDLAGQVAALDTTLAQLRRGNPARPLFLPDEPAIQAKLDLVVTDWQQRLKPLAVATASTTPATAYID
ALPVFVNEADSLVKLIERDNASKTDLLRLSQASLAIIACVGTVAVIYLLYLWIILPVLRLQDGLQRMAAREFSLRLPIAT
RDEFGTLNAGFNRMADELQELYQDLAARVDSKTAELARQNRDLEALYDMTAFLNRASDAEAMARGFLERVMRQFEADGGT
VRVIDAAHGKLHRLASVGLSDALAENSRCSAIHDCHCGAAAEAGMITVADLRQFVRQGSETQESPCGREGFRGMAAFRLD
SPRGTLGTFTLHFRNPRELPPSDRQLLQTLARHLGTALEHLRLAASARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQL
LGDAVERNDNAETREIVPLLRHGVEESYQDVRELLHNFRSRLTTGDMRPAVEDTVARFRRQCRTDARLTIEGSGPPLQPE
QQLQVLFILQEALSNVRKHAMAEHVTISLTQGRDFQLVVQDDGEGFDPSEFDGQDSAHVGLHIMQERASRLGATLTIDAR
PGAGVRVALSLPRGETMPHPDELLAAQESSQ

Sequences:

>Translated_671_residues
MLAPTNPDTTAPQPDNAHVPEAVRLTPLRHRLSTRIILLSFASLMVVLGMISGTLYLSWKLEGAGAAINDAGSLRMRANR
VAIELTRAQSGSPNDLAGQVAALDTTLAQLRRGNPARPLFLPDEPAIQAKLDLVVTDWQQRLKPLAVATASTTPATAYID
ALPVFVNEADSLVKLIERDNASKTDLLRLSQASLAIIACVGTVAVIYLLYLWIILPVLRLQDGLQRMAAREFSLRLPIAT
RDEFGTLNAGFNRMADELQELYQDLAARVDSKTAELARQNRDLEALYDMTAFLNRASDAEAMARGFLERVMRQFEADGGT
VRVIDAAHGKLHRLASVGLSDALAENSRCSAIHDCHCGAAAEAGMITVADLRQFVRQGSETQESPCGREGFRGMAAFRLD
SPRGTLGTFTLHFRNPRELPPSDRQLLQTLARHLGTALEHLRLAASARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQL
LGDAVERNDNAETREIVPLLRHGVEESYQDVRELLHNFRSRLTTGDMRPAVEDTVARFRRQCRTDARLTIEGSGPPLQPE
QQLQVLFILQEALSNVRKHAMAEHVTISLTQGRDFQLVVQDDGEGFDPSEFDGQDSAHVGLHIMQERASRLGATLTIDAR
PGAGVRVALSLPRGETMPHPDELLAAQESSQ
>Mature_671_residues
MLAPTNPDTTAPQPDNAHVPEAVRLTPLRHRLSTRIILLSFASLMVVLGMISGTLYLSWKLEGAGAAINDAGSLRMRANR
VAIELTRAQSGSPNDLAGQVAALDTTLAQLRRGNPARPLFLPDEPAIQAKLDLVVTDWQQRLKPLAVATASTTPATAYID
ALPVFVNEADSLVKLIERDNASKTDLLRLSQASLAIIACVGTVAVIYLLYLWIILPVLRLQDGLQRMAAREFSLRLPIAT
RDEFGTLNAGFNRMADELQELYQDLAARVDSKTAELARQNRDLEALYDMTAFLNRASDAEAMARGFLERVMRQFEADGGT
VRVIDAAHGKLHRLASVGLSDALAENSRCSAIHDCHCGAAAEAGMITVADLRQFVRQGSETQESPCGREGFRGMAAFRLD
SPRGTLGTFTLHFRNPRELPPSDRQLLQTLARHLGTALEHLRLAASARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQL
LGDAVERNDNAETREIVPLLRHGVEESYQDVRELLHNFRSRLTTGDMRPAVEDTVARFRRQCRTDARLTIEGSGPPLQPE
QQLQVLFILQEALSNVRKHAMAEHVTISLTQGRDFQLVVQDDGEGFDPSEFDGQDSAHVGLHIMQERASRLGATLTIDAR
PGAGVRVALSLPRGETMPHPDELLAAQESSQ

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=644, Percent_Identity=26.7080745341615, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788812, Length=250, Percent_Identity=34.4, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73564; Mature: 73564

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAPTNPDTTAPQPDNAHVPEAVRLTPLRHRLSTRIILLSFASLMVVLGMISGTLYLSWK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
LEGAGAAINDAGSLRMRANRVAIELTRAQSGSPNDLAGQVAALDTTLAQLRRGNPARPLF
ECCCCCEECCCCCCEEEECEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDEPAIQAKLDLVVTDWQQRLKPLAVATASTTPATAYIDALPVFVNEADSLVKLIERDN
CCCCCCCHHEEHHEEHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
ASKTDLLRLSQASLAIIACVGTVAVIYLLYLWIILPVLRLQDGLQRMAAREFSLRLPIAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEC
RDEFGTLNAGFNRMADELQELYQDLAARVDSKTAELARQNRDLEALYDMTAFLNRASDAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
AMARGFLERVMRQFEADGGTVRVIDAAHGKLHRLASVGLSDALAENSRCSAIHDCHCGAA
HHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCCC
AEAGMITVADLRQFVRQGSETQESPCGREGFRGMAAFRLDSPRGTLGTFTLHFRNPRELP
CCCCCEEHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCC
PSDRQLLQTLARHLGTALEHLRLAASARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHH
LGDAVERNDNAETREIVPLLRHGVEESYQDVRELLHNFRSRLTTGDMRPAVEDTVARFRR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QCRTDARLTIEGSGPPLQPEQQLQVLFILQEALSNVRKHAMAEHVTISLTQGRDFQLVVQ
HHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEE
DDGEGFDPSEFDGQDSAHVGLHIMQERASRLGATLTIDARPGAGVRVALSLPRGETMPHP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCH
DELLAAQESSQ
HHHHHCCCCCC
>Mature Secondary Structure
MLAPTNPDTTAPQPDNAHVPEAVRLTPLRHRLSTRIILLSFASLMVVLGMISGTLYLSWK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
LEGAGAAINDAGSLRMRANRVAIELTRAQSGSPNDLAGQVAALDTTLAQLRRGNPARPLF
ECCCCCEECCCCCCEEEECEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDEPAIQAKLDLVVTDWQQRLKPLAVATASTTPATAYIDALPVFVNEADSLVKLIERDN
CCCCCCCHHEEHHEEHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
ASKTDLLRLSQASLAIIACVGTVAVIYLLYLWIILPVLRLQDGLQRMAAREFSLRLPIAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEC
RDEFGTLNAGFNRMADELQELYQDLAARVDSKTAELARQNRDLEALYDMTAFLNRASDAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
AMARGFLERVMRQFEADGGTVRVIDAAHGKLHRLASVGLSDALAENSRCSAIHDCHCGAA
HHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCCC
AEAGMITVADLRQFVRQGSETQESPCGREGFRGMAAFRLDSPRGTLGTFTLHFRNPRELP
CCCCCEEHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCC
PSDRQLLQTLARHLGTALEHLRLAASARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHH
LGDAVERNDNAETREIVPLLRHGVEESYQDVRELLHNFRSRLTTGDMRPAVEDTVARFRR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QCRTDARLTIEGSGPPLQPEQQLQVLFILQEALSNVRKHAMAEHVTISLTQGRDFQLVVQ
HHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEE
DDGEGFDPSEFDGQDSAHVGLHIMQERASRLGATLTIDARPGAGVRVALSLPRGETMPHP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCH
DELLAAQESSQ
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]