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The map label for this gene is glnA [H]

Identifier: 94311000

GI number: 94311000

Start: 2234351

End: 2235766

Strand: Reverse

Name: glnA [H]

Synonym: Rmet_2062

Alternate gene names: 94311000

Gene position: 2235766-2234351 (Counterclockwise)

Preceding gene: 335055578

Following gene: 94310999

Centisome position: 56.92

GC content: 60.24

Gene sequence:

>1416_bases
ATGGCGCAGAGCGTTGCAGATGTGATGAAGCTCGTGAACGAAAACGACGTGAAGTTCGTCGATTTCCGTTTCACGGATAC
CAAGGGCAAAGAACAGCACGTCTCGGTGCCGGTGTCGCACTTCGACGAAGACAAGTTCGAGAGCGGCCACGCCTTTGATG
GTTCGTCCATCGCCGGCTGGAAGGGTATCGAGGCCTCCGACATGCTGCTCATGCCGGATTCGGGTACCGCCTACATCGAC
CCGTTCTACGAAGAGCCGACGCTGGTGCTGAGCTGCGACGTGATCGAGCCGTCGGACGGCAAGGGCTACGACCGCGATCC
GCGCTCGATCGCCAAGCGCGCTGAAGCCTACCTGAAGAGCACCGGCCTGGGCGACACCGCCTTCTTCGGTCCGGAACCCG
AGTTCTTCATCTTCGACGGCGTGACCTGGAACATCGACATGCAAGGCTGCTCGGTGAAGATCCAGTCGGAAGAAGCACCG
TGGTCGTCGGGCAAGGAATTCGAGCACGGCAACAGCGGTCACCGTCCGGGCAAGAAGGGCGGCTATTTCCCGGTTGCCCC
GATCGACACGTTCCAGGACATGCGTTCGGAAATGTGCCTGATTCTGGAATCGCTGGGCATCCCCGTTGAAGTGCACCACC
ACGAAGTGGCTGGCCAGGGCCAGAACGAAATCGGCACGCGCTTCAGCACGCTGGTGCAACGCGCCGACTGGACCCAGCTG
CAGAAGTACGTGATTCAGAACGTCGCGCACACCTACGGCAAGACGGCCACGTTCATGCCGAAGCCGATCGTGGGCGATAA
CGGTTCGGGCATGCACGTTCACCAGTCGATCTGGAAGGACGGTCAGAACCTGTTCGCGGGCAACGGCTACGCCGGCCTGT
CGGAATTCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCTCGCGCGCTGAACGCCATCACCAATCCGGGTACGAAC
TCGTACAAGCGTCTGGTGCCGGGCTTCGAGGCACCGGTGAAGCTGGCCTACTCGGCTCGCAACCGTTCGGCTTCGATCCG
TATTCCGTACGTGTCCAACCCGAAGGGTCGCCGTATCGAAACGCGCTTCCCGGATCCGCTGATGAACCCGTACCTGGGCT
TCTCGGCACTGATGATGGCCGGTCTGGATGGCGTGATGAACAAGATCCACCCGGGCGAAGCTGCCGACAAGAACCTGTAC
GATCTGCCGCCCGAAGAAGACGCGAAGATCCCGACCGTCTGCTCGAGCCTGGATCAGGCCTTGGAGTACCTGGACAACGA
CCGTGAGTTCCTGACCCGCGGTGGTGTGTTCTCGAACTCGATGCTCGACGCCTACATCGAACTGAAGATGGAAGAAGTCA
CGCGTTTCCGCATGACGACTCACCCGATTGAGTTCGACATGTACTACTCGCTGTAA

Upstream 100 bases:

>100_bases
TAACATTCGACGTCCCTTTTGGGGCGGTCCAGGCAGATGACCGCACCAACTTTGGCTTACCCACTTTCCCAGTTACAGCC
CGAATTTCCAGGAGATAGGC

Downstream 100 bases:

>100_bases
GCCGAGTGAGTTGCCGGGTTGTGGCCGGGCAACAGTTGCACCAGGAAGGGGCGGCTTCGGCTGTCCCTTTTTGTTTTGCG
GCCACGTTGATCGCTAGAAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAQSVADVMKLVNENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDSGTAYID
PFYEEPTLVLSCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDGVTWNIDMQGCSVKIQSEEAP
WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQL
QKYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVSNPKGRRIETRFPDPLMNPYLGFSALMMAGLDGVMNKIHPGEAADKNLY
DLPPEEDAKIPTVCSSLDQALEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPIEFDMYYSL

Sequences:

>Translated_471_residues
MAQSVADVMKLVNENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDSGTAYID
PFYEEPTLVLSCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDGVTWNIDMQGCSVKIQSEEAP
WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQL
QKYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVSNPKGRRIETRFPDPLMNPYLGFSALMMAGLDGVMNKIHPGEAADKNLY
DLPPEEDAKIPTVCSSLDQALEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPIEFDMYYSL
>Mature_470_residues
AQSVADVMKLVNENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDSGTAYIDP
FYEEPTLVLSCDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDGVTWNIDMQGCSVKIQSEEAPW
SSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQLQ
KYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTNS
YKRLVPGFEAPVKLAYSARNRSASIRIPYVSNPKGRRIETRFPDPLMNPYLGFSALMMAGLDGVMNKIHPGEAADKNLYD
LPPEEDAKIPTVCSSLDQALEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPIEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=64.7435897435898, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=463, Percent_Identity=24.8380129589633, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52416; Mature: 52284

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSVADVMKLVNENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGW
CCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCHHHCCCHHCCCCCCCCCCCCCCC
KGIEASDMLLMPDSGTAYIDPFYEEPTLVLSCDVIEPSDGKGYDRDPRSIAKRAEAYLKS
CCCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAFFGPEPEFFIFDGVTWNIDMQGCSVKIQSEEAPWSSGKEFEHGNSGHRPGKKG
CCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
QKYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
TRFPDPLMNPYLGFSALMMAGLDGVMNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA
CCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPIEFDMYYSL
HHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
AQSVADVMKLVNENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGW
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCHHHCCCHHCCCCCCCCCCCCCCC
KGIEASDMLLMPDSGTAYIDPFYEEPTLVLSCDVIEPSDGKGYDRDPRSIAKRAEAYLKS
CCCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAFFGPEPEFFIFDGVTWNIDMQGCSVKIQSEEAPWSSGKEFEHGNSGHRPGKKG
CCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
QKYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
TRFPDPLMNPYLGFSALMMAGLDGVMNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA
CCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPIEFDMYYSL
HHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]