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The map label for this gene is odhL [H]

Identifier: 94310986

GI number: 94310986

Start: 2216565

End: 2217989

Strand: Reverse

Name: odhL [H]

Synonym: Rmet_2048

Alternate gene names: 94310986

Gene position: 2217989-2216565 (Counterclockwise)

Preceding gene: 94310987

Following gene: 94310985

Centisome position: 56.46

GC content: 63.3

Gene sequence:

>1425_bases
ATGAGCAAACAATTCGACGTGCTGGTGATCGGCGCCGGCCCCGGCGGCTATATCGCCGCCATCCGCGCTGCCCAGTTGGG
CCTGAACGTGGCTTGCCTGGAAGGCAACGCCTATGACGATCCGAAGGGCGAAGCACGCCTGGGCGGTACCTGCCTGAACG
TGGGCTGCATTCCGTCCAAGGCACTGCTGGCTTCCTCGGAAGAGTTCGAGAACGCCTCGCATCACCTGGCCGACCATGGC
ATCACCGTGGGCGACGTCAAGGTCGACGTGGCCAAGATGCTCAAGCGCAAGGACGACATCGTCAGCAAGATGACGAAGGG
CATCGAGTTCCTGTTCCGCAAGAACAAGGTGACCCTGTTCAAGGGCTACGGCAAGTTCACCGGCAAGGCTGCAGAAGGCT
TCCAGGTGGAAGTGAATGGCGAGACGCTGACCGCCAAGCAGGTCATCATCGCCACGGGCTCGAAGGCTCGCCATCTGCCA
GGCGTCGCTGTCGACAACAACCTGATCAGCGATAACGAAGGCGCGCTGAAGTTCGGTACCGTGCCGAAGAAGCTGGGCGT
GATCGGCGCCGGCGTGATCGGCCTGGAGCTGGGCTCGGTGTGGCGCCGTCTGGGTGCCGAAGTGACCGTGCTGGAAGCGC
TGCCCGCATTCCTGGGCGCCGCCGACGAAGGCGTGGCCAAGGAAGCGCAGAAGCTGCTGACCAAGCAGGGGCTGCAGTTC
CACCTGAGCGTCAAGGTCGACGAAGTGAAGACCGGCAAGGACAACGTCACGGTCAACTACACCGACAAGGACGGCAAGGC
GCAGACGCTGGAAGTCGATCGCCTGATCGTGTCGGTGGGCCGCGTGCCGAACACCGACAACCTCGGCCTGGACGCCGTGG
GCCTGGGCGTGGACCAGCGTGGCTTCATCGAGGTGGACGACCACTGCCAGACCAAGGTGCCGGGCATCTGGGCAATCGGC
GACGTGGTGCGTGGCCCGATGCTGGCCCACAAGGCAGAAGACGAAGGCGTGGCCGTGGCCGAACGTATCGTTGGCCAGAA
GCCGCATATCGACTTCAACACGGTTCCCTGGGTTATCTACACGTTCCCGGAAATCGCGTGGGTCGGCAAGACCGAGCAGC
AACTCAAGGCCGAAGGCCGCGAGTACAAGTCGGGCCAATTCCCGTTCATGGCCAATGGCCGTGCGCTGGGCATGGGTGCT
TCGGACGGTTTCGTGAAGATGCTGGCCGATGCCAGGACCGACGAGATCCTGGGCGTGCACATCGTGGCCGCGAATGCATC
GGACCTGATCGCCGAAGCCGTGGTGGCGATGGAGTTCAAGGCTGCGAGCGAAGATATCGGTCGCGTCTGCCACCCGCACC
CGTCGATGTCGGAAGTCATGCGCGAAGCCGCGCTGGCCGTCGACAAGCGTCAGCTCAACATGTAA

Upstream 100 bases:

>100_bases
TTGTCGTCGTCCCCGCGCTTCGCGCGCGGGGACCCGGCCGCTACCTCGCCGCCGGACGCCCGCCCTTGCGCGGGCATGAC
ACACCAGAGAGAAATTTCCC

Downstream 100 bases:

>100_bases
GGCATTTGCCTCGCAAAGTGTGAGCATCGGCGCGCCGCCATTCGCGGCGCGCCGTTTTCACTTGCAGCCCCCCGGTTGAA
CCCACATGAACGTCACCGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKQFDVLVIGAGPGGYIAAIRAAQLGLNVACLEGNAYDDPKGEARLGGTCLNVGCIPSKALLASSEEFENASHHLADHG
ITVGDVKVDVAKMLKRKDDIVSKMTKGIEFLFRKNKVTLFKGYGKFTGKAAEGFQVEVNGETLTAKQVIIATGSKARHLP
GVAVDNNLISDNEGALKFGTVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKLLTKQGLQF
HLSVKVDEVKTGKDNVTVNYTDKDGKAQTLEVDRLIVSVGRVPNTDNLGLDAVGLGVDQRGFIEVDDHCQTKVPGIWAIG
DVVRGPMLAHKAEDEGVAVAERIVGQKPHIDFNTVPWVIYTFPEIAWVGKTEQQLKAEGREYKSGQFPFMANGRALGMGA
SDGFVKMLADARTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM

Sequences:

>Translated_474_residues
MSKQFDVLVIGAGPGGYIAAIRAAQLGLNVACLEGNAYDDPKGEARLGGTCLNVGCIPSKALLASSEEFENASHHLADHG
ITVGDVKVDVAKMLKRKDDIVSKMTKGIEFLFRKNKVTLFKGYGKFTGKAAEGFQVEVNGETLTAKQVIIATGSKARHLP
GVAVDNNLISDNEGALKFGTVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKLLTKQGLQF
HLSVKVDEVKTGKDNVTVNYTDKDGKAQTLEVDRLIVSVGRVPNTDNLGLDAVGLGVDQRGFIEVDDHCQTKVPGIWAIG
DVVRGPMLAHKAEDEGVAVAERIVGQKPHIDFNTVPWVIYTFPEIAWVGKTEQQLKAEGREYKSGQFPFMANGRALGMGA
SDGFVKMLADARTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
>Mature_473_residues
SKQFDVLVIGAGPGGYIAAIRAAQLGLNVACLEGNAYDDPKGEARLGGTCLNVGCIPSKALLASSEEFENASHHLADHGI
TVGDVKVDVAKMLKRKDDIVSKMTKGIEFLFRKNKVTLFKGYGKFTGKAAEGFQVEVNGETLTAKQVIIATGSKARHLPG
VAVDNNLISDNEGALKFGTVPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKLLTKQGLQFH
LSVKVDEVKTGKDNVTVNYTDKDGKAQTLEVDRLIVSVGRVPNTDNLGLDAVGLGVDQRGFIEVDDHCQTKVPGIWAIGD
VVRGPMLAHKAEDEGVAVAERIVGQKPHIDFNTVPWVIYTFPEIAWVGKTEQQLKAEGREYKSGQFPFMANGRALGMGAS
DGFVKMLADARTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=48.8222698072805, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=27.9317697228145, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI33519430, Length=444, Percent_Identity=25.9009009009009, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI33519428, Length=444, Percent_Identity=25.9009009009009, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI33519426, Length=444, Percent_Identity=25.9009009009009, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI148277065, Length=444, Percent_Identity=25.9009009009009, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI148277071, Length=444, Percent_Identity=25.9009009009009, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI22035672, Length=467, Percent_Identity=28.9079229122056, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI291045266, Length=467, Percent_Identity=25.2676659528908, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=460, Percent_Identity=24.1304347826087, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI21389617, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI65787454, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI226437568, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=40, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=31.4225053078556, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=28.6937901498929, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=497, Percent_Identity=23.7424547283702, Blast_Score=128, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=51.7241379310345, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=28.6610878661088, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=465, Percent_Identity=28.3870967741935, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=465, Percent_Identity=28.3870967741935, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=26.0606060606061, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559934, Length=201, Percent_Identity=31.3432835820896, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=47.1698113207547, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=33.1236897274633, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=27.2727272727273, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=51.7970401691332, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=27.8688524590164, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=27.8688524590164, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.0513347022587, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50547; Mature: 50415

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFDVLVIGAGPGGYIAAIRAAQLGLNVACLEGNAYDDPKGEARLGGTCLNVGCIPSK
CCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCEEEEECCCCCH
ALLASSEEFENASHHLADHGITVGDVKVDVAKMLKRKDDIVSKMTKGIEFLFRKNKVTLF
HHHCCCHHHCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KGYGKFTGKAAEGFQVEVNGETLTAKQVIIATGSKARHLPGVAVDNNLISDNEGALKFGT
ECCCCCCCCCCCCEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCCEECCCCCCEEECC
VPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKLLTKQGLQF
CCHHHHHEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
HLSVKVDEVKTGKDNVTVNYTDKDGKAQTLEVDRLIVSVGRVPNTDNLGLDAVGLGVDQR
EEEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEECCCCCC
GFIEVDDHCQTKVPGIWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDFNTVPWVIY
CCEEECCCCCCCCCCEEEEHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TFPEIAWVGKTEQQLKAEGREYKSGQFPFMANGRALGMGASDGFVKMLADARTDEILGVH
ECCCEEECCCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCHHHHHHCCCCCCEEEEE
IVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQFDVLVIGAGPGGYIAAIRAAQLGLNVACLEGNAYDDPKGEARLGGTCLNVGCIPSK
CCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCEEEEECCCCCH
ALLASSEEFENASHHLADHGITVGDVKVDVAKMLKRKDDIVSKMTKGIEFLFRKNKVTLF
HHHCCCHHHCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
KGYGKFTGKAAEGFQVEVNGETLTAKQVIIATGSKARHLPGVAVDNNLISDNEGALKFGT
ECCCCCCCCCCCCEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCCEECCCCCCEEECC
VPKKLGVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEGVAKEAQKLLTKQGLQF
CCHHHHHEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
HLSVKVDEVKTGKDNVTVNYTDKDGKAQTLEVDRLIVSVGRVPNTDNLGLDAVGLGVDQR
EEEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEECCCCCC
GFIEVDDHCQTKVPGIWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDFNTVPWVIY
CCEEECCCCCCCCCCEEEEHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TFPEIAWVGKTEQQLKAEGREYKSGQFPFMANGRALGMGASDGFVKMLADARTDEILGVH
ECCCEEECCCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCHHHHHHCCCCCCEEEEE
IVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSEVMREAALAVDKRQLNM
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]