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The map label for this gene is truB

Identifier: 94310967

GI number: 94310967

Start: 2195241

End: 2196185

Strand: Reverse

Name: truB

Synonym: Rmet_2029

Alternate gene names: 94310967

Gene position: 2196185-2195241 (Counterclockwise)

Preceding gene: 94310968

Following gene: 335055576

Centisome position: 55.91

GC content: 66.24

Gene sequence:

>945_bases
ATGACCGATTCCTCCGCGACCCGACCGCCCCGGCTGCCACGCCGCGACGTGCACGGTGTGCTGCTGCTCGACAAGCCTCT
GGGTCTGTCGTCCAACGACGCGCTGGTACGCGCCAAGCGACTGCTGCGCGCGAACAAGGCCGGCCACACGGGCACGCTGG
ATCCGCTGGCGACTGGACTGCTGCCGCTGTGTTTTGGCGAGGCCACCAAGTTCTCTCAGGATCTGCTTGACGCGGACAAG
ACCTACGAGGCGAAAGTCCGGCTTGGCGCGACGACGACCACAGGCGATGCCGAGGGCGAGATCGTCGTCGAGCGACTGGT
GACATGCGATCACGACGCGCTTGATGCTGCGGTTGCGCGCTTTACCGGAGAGATCGAACAGGTACCGCCGATGTACTCGG
CACTCAAGAAGGACGGCAAGCCGCTTTACGAGTATGCCCGGGCCGGGCAGACCGTCGAGCGCGCGGCGCGGCAGGTAACG
ATTCACGCCATCACGCTGCTGGACGTTGATTTGCCTGCGGCGATGTTCACGATGCGTGTCACATGCAGCAAGGGCACGTA
CATTCGCACGCTGGCCGAAGATGTCGGCGAGGCGCTGGGTTGCGGCGCGCACCTGATTGGGCTGCGCCGAACCGCCGTCG
GTGACCTGACGCTGGAAGGCGCGGTCACGCTCGAGCAGATTGACGCACAGGCTGACGAAGCACGGCCGGGAATGCTCGCG
CCTGTTGACGCGCTGCTGCAACGGTGCCCGCCGGTGCATCTCGATGCCGCCGCGATGGGGCGCTTTCTGCAAGGCCAGCG
CATCGCACGCCGCGATCTGCCGGAAGGGCAGGTGCCGCCGGATGAGGGCACGCTGGCACGTGTCTATGGCGACGAAGGGC
GTCTGCTAGGCGTCGCGCGGATGAAGGAAGGGGCCTTGCGGCCCGAGCGGCTAGTCAAACTCTAG

Upstream 100 bases:

>100_bases
TCAACGAAGCGAACGCTACGCGCGCCAAGGACGACGAGTAAGTCCACGGGTGCCCAAGTGGTGCCGGTGGATGCCGGCAC
CACGTTTTTCCTCTCCTGCA

Downstream 100 bases:

>100_bases
GACGCGGCACAAGCAACGCCCAAGTAAAAAAGGCATCGGATAAACCCGATGCCTTTTTTGTTCGATGTTCGACGTCAGTG
CGCTCCGGCTGCTTCCGCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTDSSATRPPRLPRRDVHGVLLLDKPLGLSSNDALVRAKRLLRANKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYEAKVRLGATTTTGDAEGEIVVERLVTCDHDALDAAVARFTGEIEQVPPMYSALKKDGKPLYEYARAGQTVERAARQVT
IHAITLLDVDLPAAMFTMRVTCSKGTYIRTLAEDVGEALGCGAHLIGLRRTAVGDLTLEGAVTLEQIDAQADEARPGMLA
PVDALLQRCPPVHLDAAAMGRFLQGQRIARRDLPEGQVPPDEGTLARVYGDEGRLLGVARMKEGALRPERLVKL

Sequences:

>Translated_314_residues
MTDSSATRPPRLPRRDVHGVLLLDKPLGLSSNDALVRAKRLLRANKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYEAKVRLGATTTTGDAEGEIVVERLVTCDHDALDAAVARFTGEIEQVPPMYSALKKDGKPLYEYARAGQTVERAARQVT
IHAITLLDVDLPAAMFTMRVTCSKGTYIRTLAEDVGEALGCGAHLIGLRRTAVGDLTLEGAVTLEQIDAQADEARPGMLA
PVDALLQRCPPVHLDAAAMGRFLQGQRIARRDLPEGQVPPDEGTLARVYGDEGRLLGVARMKEGALRPERLVKL
>Mature_313_residues
TDSSATRPPRLPRRDVHGVLLLDKPLGLSSNDALVRAKRLLRANKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADKT
YEAKVRLGATTTTGDAEGEIVVERLVTCDHDALDAAVARFTGEIEQVPPMYSALKKDGKPLYEYARAGQTVERAARQVTI
HAITLLDVDLPAAMFTMRVTCSKGTYIRTLAEDVGEALGCGAHLIGLRRTAVGDLTLEGAVTLEQIDAQADEARPGMLAP
VDALLQRCPPVHLDAAAMGRFLQGQRIARRDLPEGQVPPDEGTLARVYGDEGRLLGVARMKEGALRPERLVKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=36.9369369369369, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4503337, Length=214, Percent_Identity=31.3084112149533, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI215599015, Length=214, Percent_Identity=31.3084112149533, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=47.7707006369427, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17553978, Length=259, Percent_Identity=27.4131274131274, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=34.375, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=243, Percent_Identity=32.0987654320988, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281364189, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364187, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364185, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364183, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI62471759, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17975520, Length=211, Percent_Identity=29.8578199052133, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RALME (Q1LLR8)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584177.1
- ProteinModelPortal:   Q1LLR8
- SMR:   Q1LLR8
- STRING:   Q1LLR8
- GeneID:   4038836
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2029
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q1LLR8
- ProtClustDB:   PRK01851
- BioCyc:   RMET266264:RMET_2029-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33803; Mature: 33672

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSATRPPRLPRRDVHGVLLLDKPLGLSSNDALVRAKRLLRANKAGHTGTLDPLATGL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYEAKVRLGATTTTGDAEGEIVVERLVTCDHDALDAAVAR
HHHHHCCHHHHHHHHHCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHCCCHHHHHHHHH
FTGEIEQVPPMYSALKKDGKPLYEYARAGQTVERAARQVTIHAITLLDVDLPAAMFTMRV
HHCCHHHCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHEEEEEEEEEECCCCHHHEEEEE
TCSKGTYIRTLAEDVGEALGCGAHLIGLRRTAVGDLTLEGAVTLEQIDAQADEARPGMLA
EECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCEEHHHHHCCHHHCCCCCCC
PVDALLQRCPPVHLDAAAMGRFLQGQRIARRDLPEGQVPPDEGTLARVYGDEGRLLGVAR
CHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEHHH
MKEGALRPERLVKL
HHCCCCCHHHHHCC
>Mature Secondary Structure 
TDSSATRPPRLPRRDVHGVLLLDKPLGLSSNDALVRAKRLLRANKAGHTGTLDPLATGL
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYEAKVRLGATTTTGDAEGEIVVERLVTCDHDALDAAVAR
HHHHHCCHHHHHHHHHCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHCCCHHHHHHHHH
FTGEIEQVPPMYSALKKDGKPLYEYARAGQTVERAARQVTIHAITLLDVDLPAAMFTMRV
HHCCHHHCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHEEEEEEEEEECCCCHHHEEEEE
TCSKGTYIRTLAEDVGEALGCGAHLIGLRRTAVGDLTLEGAVTLEQIDAQADEARPGMLA
EECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCEEHHHHHCCHHHCCCCCCC
PVDALLQRCPPVHLDAAAMGRFLQGQRIARRDLPEGQVPPDEGTLARVYGDEGRLLGVAR
CHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEHHH
MKEGALRPERLVKL
HHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA