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The map label for this gene is citA [H]

Identifier: 94310887

GI number: 94310887

Start: 2119836

End: 2121125

Strand: Direct

Name: citA [H]

Synonym: Rmet_1949

Alternate gene names: 94310887

Gene position: 2119836-2121125 (Clockwise)

Preceding gene: 94310886

Following gene: 94310888

Centisome position: 53.97

GC content: 62.95

Gene sequence:

>1290_bases
ATGTCCAGCACTACCCAGTCACAACACGGATTCCGCACCGTGTTCCGCGTTGTCAGCGGCAACTTCCTGGAAATGTACGA
CTTCATGGTCTATGGCTTTTACGCTGCAGCCATCGCCCGTACGTTCTTTCCTTCGGATAATGAATTCGCATCGCTGATGC
TCTCGCTGGCCACGTTCGGCGCGGGCTTCCTGATGCGACCATTGGGCGCCATCATCCTGGGCGCCTATATCGATCACCAC
GGGCGTCGCAAAGGCCTGATCCTCACGCTGATCCTGATGGCGGCCGGCACGCTGCTCATTGCGGTAGTGCCAGGGTACGC
GGCGATCGGCGTGGCCGCGCCGCTGCTAGTCCTGTTCGGACGACTGCTGCAGGGCTTCTCCGCAGGCGTGGAACTGGGCG
GTGTATCGGTGTATCTGTCCGAGATCGCCAAGCCCGGCAAGAAGGGCTTCTACGTGGCATGGCAATCGGCCAGCCAGCAG
GTAGCCGTGATCTTCGCGGGCCTGCTCGGCGTGATCCTGCACGCCACACTGGCGCCGGCGCAGATGGATAGCTGGGGCTG
GCGTGTGCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCATCATCCGCCGCTCGCTCGAGGAGACCGAGGAGT
TCCGCACGCGCAAGCATCGTCCGAGCGTGAACGAGATCTATCGTTCGATGCTCGACAACTGGGGCATCATCATCGCCGGC
TGCATGATGGTGGTGATGACCACGGTGTCGTTCTACATGATCACCGCCTACACGCCGACCTTCGGCAAGACCGTGCTGAA
GCTCGACGACGTGGACAACCTGATCGTCACGATGTGCGTCGGCCTGTCGAACTTTATCTGGCTGCCGCTGATGGGCGCGG
TGTCCGACCGCATTGGCCGCAAGCCGCTGCTGGTGTTCTTCACGATCGCCACGCTGGTGACCGCCTACCCCGCCGTTTCG
TGGCTGGTGGCCGCCCCGTCGTTCGAACGCCTGCTGATGGTGGAGCTGTGGCTCTCCTTCCTGTACGGCAGCTATAACGG
CGCAATGGTCGTCACGCTGACCGAAGTCATGCCGCCCGCCGTGCGCACCGCCGGCTTCTCGCTGGCATACAGCCTGGCCA
CGGCACTGTTCGGCGGCTTTACGCCAGCGATCTCGACCTACCTGATCCACGCCACGGGCAACAAGGCCGCGCCGGGTCTG
TGGCTGATGTTCGCCGCCGCATGCGGTCTGGTTGCCACGATCATCATCTTCCGCACACGTCGCGAGCAGTCCGCGCTGAG
CGCCGCATAA

Upstream 100 bases:

>100_bases
GCATCCCTAGCCATGGCACCACCTGCCGCAACCCCAAACGCCCTGGCTGCCCACACCGAAAACATACCGCCGGTCAACCG
GCGGTGATCGAGAGACAGCC

Downstream 100 bases:

>100_bases
CATCACGTGGCACGTATCGCGCGTCACCTGACGCCACACGCCGCCTTCACATTCCTGCCAAATCGCATAGCAGGCAAGCG
TGAAGGCGGCGTGACCCGTT

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSTTQSQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSDNEFASLMLSLATFGAGFLMRPLGAIILGAYIDHH
GRRKGLILTLILMAAGTLLIAVVPGYAAIGVAAPLLVLFGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQSASQQ
VAVIFAGLLGVILHATLAPAQMDSWGWRVPFLIGCLIVPFLFIIRRSLEETEEFRTRKHRPSVNEIYRSMLDNWGIIIAG
CMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTIATLVTAYPAVS
WLVAAPSFERLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAISTYLIHATGNKAAPGL
WLMFAAACGLVATIIIFRTRREQSALSAA

Sequences:

>Translated_429_residues
MSSTTQSQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSDNEFASLMLSLATFGAGFLMRPLGAIILGAYIDHH
GRRKGLILTLILMAAGTLLIAVVPGYAAIGVAAPLLVLFGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQSASQQ
VAVIFAGLLGVILHATLAPAQMDSWGWRVPFLIGCLIVPFLFIIRRSLEETEEFRTRKHRPSVNEIYRSMLDNWGIIIAG
CMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTIATLVTAYPAVS
WLVAAPSFERLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAISTYLIHATGNKAAPGL
WLMFAAACGLVATIIIFRTRREQSALSAA
>Mature_428_residues
SSTTQSQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSDNEFASLMLSLATFGAGFLMRPLGAIILGAYIDHHG
RRKGLILTLILMAAGTLLIAVVPGYAAIGVAAPLLVLFGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQSASQQV
AVIFAGLLGVILHATLAPAQMDSWGWRVPFLIGCLIVPFLFIIRRSLEETEEFRTRKHRPSVNEIYRSMLDNWGIIIAGC
MMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTIATLVTAYPAVSW
LVAAPSFERLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAISTYLIHATGNKAAPGLW
LMFAAACGLVATIIIFRTRREQSALSAA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=416, Percent_Identity=29.3269230769231, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788942, Length=368, Percent_Identity=36.1413043478261, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=28.2710280373832, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=413, Percent_Identity=29.0556900726392, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87082231, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46685; Mature: 46554

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCC
>Mature Secondary Structure 
SSTTQSQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSDNEFASLMLSLATFG
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC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HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]