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The map label for this gene is pcaR [H]

Identifier: 94310868

GI number: 94310868

Start: 2098060

End: 2098845

Strand: Direct

Name: pcaR [H]

Synonym: Rmet_1930

Alternate gene names: 94310868

Gene position: 2098060-2098845 (Clockwise)

Preceding gene: 94310862

Following gene: 94310870

Centisome position: 53.41

GC content: 64.5

Gene sequence:

>786_bases
ATGACCACCGACGACAAAGAACGCCCCGACCCGCTGTTCAACCAGTCCCTTGAAAAGGGCCTCGAAGTACTGCGTGCATT
CAGCGCCGTGCATCGCACGCTGACGTTGGCCGAGCTGGCAGCACTGACAGGCATGACCAAGAGCTCCGCCCAGCGCACGA
TCCACACGCTGGAGCGGCTCGGGTACGTCGACAAGCATCCCCAGACGAGGCGTTTCCGGCTGACGCCCAGAGTGATGGAG
ATCGGCTACAACTACCTTGCCGCAGACGCCCTGATTCCGATCGCCAGCCCCTACCTCTCGCAACTGGCCAATGCCAGCGG
CGAGACCGCCAACCTGACCGAGCCTGTCGGCCTGGAGATGGTCTACATGGCGCAGCTCGTGACCGCCAAGCACATGCCAG
TGCTGACGCCGGTGGGCATGCGGATTCCGATGTACTGCACAAGCTCGGGACGCGCCTACCTGAGCACGCTGCCGGACGAA
CGTGTGCGAGCGATGCTTGAAGCGTCTGCGCGCACGCCACGCACGCCGGTCACGCTGACCGACGTCGAAACCATCTTCGA
GCGCGTCGTGGCGTGCCGCACGGTCGGTTACGCGTGCAACGAGGAGGAACTGTTCCTGGGCGATATGGGAATTGCCGCGC
CGGTGGTGAACAGCCGTGGCGAGGCAGTGGGAGCGGTCCATGTTTCGCCACCAGCCAGCCGCTGGACCATGGTCGACGCG
CAGAAAAAGCTCGGCCCGCTGGTGGTCGAATGCGCGTGGGCGATCTCGCGATCGATCGCCCACTGA

Upstream 100 bases:

>100_bases
GGTCTGAAATCGATAATCGATTCGACCGCATCTTGGTGCAATACATCTACATCGTGGTGCAGCGGGTCACGCCGATGGTG
CCAACTTCAGGTCCAAGACC

Downstream 100 bases:

>100_bases
GCCGGCAGGCGCTTATCGCGCGTGTTCCAGCATGGCTCGCAGCAGGACGTTGGCACCGGCTTCGATATGCTCGGGCAGCG
CGTCCTCCAGCTCGTTATGG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERLGYVDKHPQTRRFRLTPRVME
IGYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEMVYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDE
RVRAMLEASARTPRTPVTLTDVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDA
QKKLGPLVVECAWAISRSIAH

Sequences:

>Translated_261_residues
MTTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERLGYVDKHPQTRRFRLTPRVME
IGYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEMVYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDE
RVRAMLEASARTPRTPVTLTDVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDA
QKKLGPLVVECAWAISRSIAH
>Mature_260_residues
TTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERLGYVDKHPQTRRFRLTPRVMEI
GYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEMVYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDER
VRAMLEASARTPRTPVTLTDVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDAQ
KKLGPLVVECAWAISRSIAH

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=258, Percent_Identity=30.2325581395349, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1786468, Length=220, Percent_Identity=27.7272727272727, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082359, Length=244, Percent_Identity=27.0491803278689, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788131, Length=233, Percent_Identity=26.1802575107296, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28599; Mature: 28468

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GYVDKHPQTRRFRLTPRVMEIGYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEM
CCCCCCCCCCEEECCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHH
VYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDERVRAMLEASARTPRTPVTLT
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEHH
DVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDA
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCEEEEEEECCCCCCEEEECC
QKKLGPLVVECAWAISRSIAH
HHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GYVDKHPQTRRFRLTPRVMEIGYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEM
CCCCCCCCCCEEECCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHH
VYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDERVRAMLEASARTPRTPVTLT
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEHH
DVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDA
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCEEEEEEECCCCCCEEEECC
QKKLGPLVVECAWAISRSIAH
HHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]