BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is gra-orf6 [H]

Identifier: 94310849

GI number: 94310849

Start: 2080257

End: 2081006

Strand: Reverse

Name: gra-orf6 [H]

Synonym: Rmet_1911

Alternate gene names: 94310849

Gene position: 2081006-2080257 (Counterclockwise)

Preceding gene: 94310850

Following gene: 94310848

Centisome position: 52.98

GC content: 66.4

Gene sequence:

>750_bases
ATGCCTATTCATTCCCTATCCGGCAAAGTCGCCGTGATCATCGGTGGCACCGGCGGCATTGGCCTCGCCACAGGCCATGC
GCTGGCAGAGATCGGCGCCAGCGTGGTGCTGACCGGACGCGATGCGGACCGCACCGCGAGCGCCGTCGCCACGCTGCCGG
CCGGCAATCACCTGGCTGCTCATGCAGAAGTCGGCGACAGCGCCACGCTGCGGCTGCTTGCGGACGACGTCCAGCATCAT
TACGGACGTGTCGACATCCTCGTCAACACGTCGGGCTTCACACGTGCGATTCCGCATGCCGATCTCGATGCGCTCGACGA
TGCGTTGATCGACGAAATGTTTGCCGTCAACTGGCGCGGCCAGTTCGCCGCGATTCGCGCGTTCGCACCACTGCTGCGCG
CGAACGGCGACGGCCTGGTCGTCAACGTCGGCTCGATCGCGGGGCGCACCGGGCAAGGCAGCAACGTTGCGTACTGCGCG
GTCAAGGCGGGTCTCGACGTCATGGCAATGTCGCTGGGCCGTGCACTCGCCCCAGCAATCCGCGTGCTCAATGTTTCTCC
GGGCGTAGTGGATACCGGCTTCGTACCGGGCCGCGATAGCAGCTTCAATGAACGCGCGGCCAAGACCACACCGCTGCGGC
GGATCGGCCAGGCGCAGGACGTGGCTGACGCCATCGTCGCGTGCGCCACATCGCTTCGCTTTGCCACGGGCACCACCATC
GTCGTCGATGGCGGGCGCCAGCTCGGCTGA

Upstream 100 bases:

>100_bases
GTTCAGACGTTCCCGCGTCCGCCGATCGATTGATGGACCGACGAACCCGTCGCTCCACCGTCGCAACGTTTATCACGACC
TTCCATTCATCCAACTGAAC

Downstream 100 bases:

>100_bases
TTCCTTTTCTTCTCAGTTCAACGTATCCATGCACGCACGCAAGACCATCATCACCTGCGCGGTGACCGGCAATATCGTCA
AGCCAGACCAGCATCCCGGT

Product: NAD-dependent epimerase/dehydratase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORF6 [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAAHAEVGDSATLRLLADDVQHH
YGRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRGQFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCA
VKAGLDVMAMSLGRALAPAIRVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
VVDGGRQLG

Sequences:

>Translated_249_residues
MPIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAAHAEVGDSATLRLLADDVQHH
YGRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRGQFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCA
VKAGLDVMAMSLGRALAPAIRVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
VVDGGRQLG
>Mature_248_residues
PIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAAHAEVGDSATLRLLADDVQHHY
GRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRGQFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCAV
KAGLDVMAMSLGRALAPAIRVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTIV
VDGGRQLG

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=30.8943089430894, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI32483357, Length=248, Percent_Identity=31.0483870967742, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=29.0196078431373, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=29.8039215686275, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI142976729, Length=239, Percent_Identity=25.5230125523013, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=27.7992277992278, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=26.2931034482759, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI126723750, Length=249, Percent_Identity=28.5140562248996, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=28.8617886178862, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=26.1904761904762, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=30.6451612903226, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI83715985, Length=187, Percent_Identity=31.5508021390374, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787905, Length=251, Percent_Identity=27.8884462151394, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=32.03125, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367175, Length=251, Percent_Identity=29.8804780876494, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=29.8449612403101, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=27.710843373494, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=26.6932270916335, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=26.4367816091954, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787820, Length=223, Percent_Identity=25.5605381165919, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=31.4516129032258, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=29.0441176470588, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=31.6205533596838, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=252, Percent_Identity=33.7301587301587, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=32.5757575757576, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=29.7709923664122, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=32.8301886792453, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=30.241935483871, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=31.4393939393939, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=31.3207547169811, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=243, Percent_Identity=28.8065843621399, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=32.2344322344322, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=267, Percent_Identity=29.5880149812734, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=31.8518518518519, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=31.5985130111524, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=264, Percent_Identity=32.1969696969697, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=28.125, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=266, Percent_Identity=29.3233082706767, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=29.7709923664122, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.9242424242424, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=27.5471698113208, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=30.9433962264151, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=257, Percent_Identity=28.0155642023346, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=205, Percent_Identity=28.2926829268293, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=27.8625954198473, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=174, Percent_Identity=25.8620689655172, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=29.2, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=230, Percent_Identity=27.3913043478261, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=245, Percent_Identity=30.2040816326531, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=25.8687258687259, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355319, Length=250, Percent_Identity=30.8, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=31.3492063492063, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639444, Length=258, Percent_Identity=27.906976744186, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357041, Length=261, Percent_Identity=29.5019157088123, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=30.46875, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571526, Length=257, Percent_Identity=28.0155642023346, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24644337, Length=255, Percent_Identity=30.9803921568627, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643142, Length=245, Percent_Identity=32.2448979591837, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17737361, Length=273, Percent_Identity=27.8388278388278, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364892, Length=205, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25379; Mature: 25248

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEECCCCCCEEE
HAEVGDSATLRLLADDVQHHYGRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRG
ECCCCCCCEEHHHHHHHHHHCCEEEEEEECCCCEECCCCCCHHHHHHHHHHHHHHCCCCC
QFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCAVKAGLDVMAMSLGRALAPAI
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
RVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
EEEECCCCCEECCCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCEE
VVDGGRQLG
EEECCCCCC
>Mature Secondary Structure 
PIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAA
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEECCCCCCEEE
HAEVGDSATLRLLADDVQHHYGRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRG
ECCCCCCCEEHHHHHHHHHHCCEEEEEEECCCCEECCCCCCHHHHHHHHHHHHHHCCCCC
QFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCAVKAGLDVMAMSLGRALAPAI
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
RVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
EEEECCCCCEECCCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCEE
VVDGGRQLG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 9831526 [H]