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The map label for this gene is poxB [H]

Identifier: 94310837

GI number: 94310837

Start: 2067195

End: 2068907

Strand: Reverse

Name: poxB [H]

Synonym: Rmet_1899

Alternate gene names: 94310837

Gene position: 2068907-2067195 (Counterclockwise)

Preceding gene: 335055563

Following gene: 94310835

Centisome position: 52.67

GC content: 61.24

Gene sequence:

>1713_bases
ATGGGCAAGAAAGTTGCGGAGATCATCGTAGAGGTACTCGAACAGGCTGGCGTCAAGCGCTGCTATGGCATTGTCGGCGA
CACCCTCAACCTGATTGCCGAACACCTCGATCGAAGTTCCATCGACTGGGTCGGTGTTCGTCACGAAGAAGCGGGCGCCT
TCGCGGCAGGCGCTGAGGCGCTATACACCAACAACCTGACAGCGTGCGCCGGAAGCTGCGGCCCAGGCAGTCTGCACTTC
ATCAACAGCCTGTACGAGGCCAATCGCAATCGCGCTCCGGTTGTCCTGATCGCCAGTCAGGTAGCGATGAGCGAGATGGG
CTTCGAGTTCATTCAAGAGGTGGATTTCAAGTCGGTCTTCAAGGAATGCTCGGTGTTTTGCGACATGATCCTGACGCCCG
AACAGGCCCGCAGGAAAACCGCGATGGCCTGTCAGACCGCACTGACCAGGCGCGGCGTTGCCGTGTTGATCGTGCCGGTG
GACATTTCACATGGCGAAGTGAAGGAGGACTTCCCTTATCGCGTTCACCACAGTCGTCCATCCATCCGGCCGAATGACGC
GGAGATCGGAGCCATCGCAGAGGTCCTGAATGCCGGCAGGAAGACTGCCATTTATGCGGGGTCCGGCTGCGAGCAGGCGC
TCGCCGAGATCATGGCCGTCGCGGAAAGGCTTAAGGCGCCGGTCGCCCATACCTCGCGCGCGAAGAACTGGCTCGAACCG
GGCAATCCGTACAACATCGGCATGACGGGCATCCTGGGGAACGAAGCCGGCTATAACGCGGTGCTCGAATGCGACACGCT
GCTGTTGCTCGGCGCGGATTTTGCGTGGCGCCAGTTCTATCCCGATGGGGCAAAGATCGTGCAGATCGACATCGACCCAA
CGCATCTGGGCCGACGCCACCCCATCACGCTGGGCGCGGTAGGCGACATCCAAACTACGCTGCAGGCATTGCTGCCGCAT
CTGGACCAGCACGAGGACGCGTCATTCCAGGAGAAATACATCAAGCGTTATGCCGAAGCGATGGTAGCGGCAAGGAAGCC
TCTCATCCCGGGCCACAACGACAGCATTCCCGGCTCGTACCTCGCGTCAATCATCGACAAGCACGCCGCCGACGATGCGC
TGATCTGCGCTGACGATGGCAGTCCGATCGTGTGGGCGTTGCGTCTGTTCGACGCCAACGGCAAACGCCAGATCTTCGGC
AGCCTCCTGCACGGCACCATGGCGAGCGCCTTGCCCAGCGCACTGGGCCTGCAGAAGGCATGCCCCGATCGCGAGGTCAT
CGCCATGTGCGGCGACGGCGGCATTACGATGCTGTTCGGCGAACTGCTCACCACGATCCAGGAAGAGGTGCCCATCAAGA
TCGTGGTGTTCGACAACAGCAAGCTTGGCTTCGTGGAGATCGAGCAGAAGGCCGAGGGCATGCTCGACACCTTTACGAAA
CTGAAGAACCCAGACTTCGGCAAGGTGGCGGAGGCCATCGGCCTCTGGGGCAAGACGGTGCGCAAGGCCTCTGAGCTGGA
CGCGTCGGTGCAGGAGTGGCTGTCGCAACCCGGCCCTGCGCTTCTCAATGTCATCGTCAATCCGCTGGAACTGGTCAAGC
CACCCTTCCTGGAAGCCGGCCCCGTGATCGGCATGGCGATGTACTCCGTGCGCGCCATCCTCAACGGACGCGGCGCGGAT
GTACTGGAAATGGTGCGCGAGAACTTCCGCTGA

Upstream 100 bases:

>100_bases
CGAACCAAAGAATGTTCGTCGATCTCCGGGGCCATAGGGGTGTCAAGATGGCGAAGAGCACCCTCGCCAGCTTTCCAGTT
CCAGCGTAGAGGAGATAAGA

Downstream 100 bases:

>100_bases
AGACCCAAGCGTCGGACCCAAGCGTTGCTTCAGGTAAGCAACGTCTTGGGCTCCAGGTACGCGCTCATGCCCCATCGGCC
CATCTCTCGCCCGAGCCCGG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MGKKVAEIIVEVLEQAGVKRCYGIVGDTLNLIAEHLDRSSIDWVGVRHEEAGAFAAGAEALYTNNLTACAGSCGPGSLHF
INSLYEANRNRAPVVLIASQVAMSEMGFEFIQEVDFKSVFKECSVFCDMILTPEQARRKTAMACQTALTRRGVAVLIVPV
DISHGEVKEDFPYRVHHSRPSIRPNDAEIGAIAEVLNAGRKTAIYAGSGCEQALAEIMAVAERLKAPVAHTSRAKNWLEP
GNPYNIGMTGILGNEAGYNAVLECDTLLLLGADFAWRQFYPDGAKIVQIDIDPTHLGRRHPITLGAVGDIQTTLQALLPH
LDQHEDASFQEKYIKRYAEAMVAARKPLIPGHNDSIPGSYLASIIDKHAADDALICADDGSPIVWALRLFDANGKRQIFG
SLLHGTMASALPSALGLQKACPDREVIAMCGDGGITMLFGELLTTIQEEVPIKIVVFDNSKLGFVEIEQKAEGMLDTFTK
LKNPDFGKVAEAIGLWGKTVRKASELDASVQEWLSQPGPALLNVIVNPLELVKPPFLEAGPVIGMAMYSVRAILNGRGAD
VLEMVRENFR

Sequences:

>Translated_570_residues
MGKKVAEIIVEVLEQAGVKRCYGIVGDTLNLIAEHLDRSSIDWVGVRHEEAGAFAAGAEALYTNNLTACAGSCGPGSLHF
INSLYEANRNRAPVVLIASQVAMSEMGFEFIQEVDFKSVFKECSVFCDMILTPEQARRKTAMACQTALTRRGVAVLIVPV
DISHGEVKEDFPYRVHHSRPSIRPNDAEIGAIAEVLNAGRKTAIYAGSGCEQALAEIMAVAERLKAPVAHTSRAKNWLEP
GNPYNIGMTGILGNEAGYNAVLECDTLLLLGADFAWRQFYPDGAKIVQIDIDPTHLGRRHPITLGAVGDIQTTLQALLPH
LDQHEDASFQEKYIKRYAEAMVAARKPLIPGHNDSIPGSYLASIIDKHAADDALICADDGSPIVWALRLFDANGKRQIFG
SLLHGTMASALPSALGLQKACPDREVIAMCGDGGITMLFGELLTTIQEEVPIKIVVFDNSKLGFVEIEQKAEGMLDTFTK
LKNPDFGKVAEAIGLWGKTVRKASELDASVQEWLSQPGPALLNVIVNPLELVKPPFLEAGPVIGMAMYSVRAILNGRGAD
VLEMVRENFR
>Mature_569_residues
GKKVAEIIVEVLEQAGVKRCYGIVGDTLNLIAEHLDRSSIDWVGVRHEEAGAFAAGAEALYTNNLTACAGSCGPGSLHFI
NSLYEANRNRAPVVLIASQVAMSEMGFEFIQEVDFKSVFKECSVFCDMILTPEQARRKTAMACQTALTRRGVAVLIVPVD
ISHGEVKEDFPYRVHHSRPSIRPNDAEIGAIAEVLNAGRKTAIYAGSGCEQALAEIMAVAERLKAPVAHTSRAKNWLEPG
NPYNIGMTGILGNEAGYNAVLECDTLLLLGADFAWRQFYPDGAKIVQIDIDPTHLGRRHPITLGAVGDIQTTLQALLPHL
DQHEDASFQEKYIKRYAEAMVAARKPLIPGHNDSIPGSYLASIIDKHAADDALICADDGSPIVWALRLFDANGKRQIFGS
LLHGTMASALPSALGLQKACPDREVIAMCGDGGITMLFGELLTTIQEEVPIKIVVFDNSKLGFVEIEQKAEGMLDTFTKL
KNPDFGKVAEAIGLWGKTVRKASELDASVQEWLSQPGPALLNVIVNPLELVKPPFLEAGPVIGMAMYSVRAILNGRGADV
LEMVRENFR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=23.8010657193606, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=42.3817863397548, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=27.2727272727273, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=27.3743016759777, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786717, Length=482, Percent_Identity=25.103734439834, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=22.2426470588235, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=25.7495590828924, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=25.7495590828924, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=24.2477876106195, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320816, Length=562, Percent_Identity=22.9537366548043, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922626, Length=561, Percent_Identity=21.74688057041, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61795; Mature: 61664

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKVAEIIVEVLEQAGVKRCYGIVGDTLNLIAEHLDRSSIDWVGVRHEEAGAFAAGAEA
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHH
LYTNNLTACAGSCGPGSLHFINSLYEANRNRAPVVLIASQVAMSEMGFEFIQEVDFKSVF
HHCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHH
KECSVFCDMILTPEQARRKTAMACQTALTRRGVAVLIVPVDISHGEVKEDFPYRVHHSRP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCEEEECCCC
SIRPNDAEIGAIAEVLNAGRKTAIYAGSGCEQALAEIMAVAERLKAPVAHTSRAKNWLEP
CCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
GNPYNIGMTGILGNEAGYNAVLECDTLLLLGADFAWRQFYPDGAKIVQIDIDPTHLGRRH
CCCCCCCEEEEECCCCCCCEEEECCEEEEECCCHHHHHHCCCCCEEEEEECCHHHCCCCC
PITLGAVGDIQTTLQALLPHLDQHEDASFQEKYIKRYAEAMVAARKPLIPGHNDSIPGSY
CEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LASIIDKHAADDALICADDGSPIVWALRLFDANGKRQIFGSLLHGTMASALPSALGLQKA
HHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHC
CPDREVIAMCGDGGITMLFGELLTTIQEEVPIKIVVFDNSKLGFVEIEQKAEGMLDTFTK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHHHHHHH
LKNPDFGKVAEAIGLWGKTVRKASELDASVQEWLSQPGPALLNVIVNPLELVKPPFLEAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCCCC
PVIGMAMYSVRAILNGRGADVLEMVRENFR
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GKKVAEIIVEVLEQAGVKRCYGIVGDTLNLIAEHLDRSSIDWVGVRHEEAGAFAAGAEA
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHH
LYTNNLTACAGSCGPGSLHFINSLYEANRNRAPVVLIASQVAMSEMGFEFIQEVDFKSVF
HHCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHH
KECSVFCDMILTPEQARRKTAMACQTALTRRGVAVLIVPVDISHGEVKEDFPYRVHHSRP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCEEEECCCC
SIRPNDAEIGAIAEVLNAGRKTAIYAGSGCEQALAEIMAVAERLKAPVAHTSRAKNWLEP
CCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
GNPYNIGMTGILGNEAGYNAVLECDTLLLLGADFAWRQFYPDGAKIVQIDIDPTHLGRRH
CCCCCCCEEEEECCCCCCCEEEECCEEEEECCCHHHHHHCCCCCEEEEEECCHHHCCCCC
PITLGAVGDIQTTLQALLPHLDQHEDASFQEKYIKRYAEAMVAARKPLIPGHNDSIPGSY
CEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LASIIDKHAADDALICADDGSPIVWALRLFDANGKRQIFGSLLHGTMASALPSALGLQKA
HHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHC
CPDREVIAMCGDGGITMLFGELLTTIQEEVPIKIVVFDNSKLGFVEIEQKAEGMLDTFTK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHHHHHHH
LKNPDFGKVAEAIGLWGKTVRKASELDASVQEWLSQPGPALLNVIVNPLELVKPPFLEAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCCCC
PVIGMAMYSVRAILNGRGADVLEMVRENFR
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]