Click here to switch to the map view.

The map label for this gene is alkK [H]

Identifier: 94310801

GI number: 94310801

Start: 2019748

End: 2021352

Strand: Reverse

Name: alkK [H]

Synonym: Rmet_1863

Alternate gene names: 94310801

Gene position: 2021352-2019748 (Counterclockwise)

Preceding gene: 94310808

Following gene: 94310800

Centisome position: 51.46

GC content: 63.61

Gene sequence:

>1605_bases
ATGAGCGCACCGTTGCTCATTTCCTCCATCATCCGGCACGCTGCGCGCTACTACGGCAGCACCGAAATCGTCTCGCGACG
AACCGAAGGCGACCTGCATCGCTATACCTACCGCGATTGCGAACTGCGCGCGCGCAAGCTGGCACAGGCGCTTGGCGCGC
TGGGCGTGCAGCAGGGCGAACGCGTTGGCACGCTGGCCTGGAACGGCTATCGCCACCTTGAGATTTACTACGGCGTGTCC
GGCATGGGCGCCGTCTGTCATACGATCAACCCGCGCCTGTTCCCGGAACAGATCGCCTACATCGTCAATCATGCCGACGA
CCAGTACGTGTTCTTCGACAGCACGTTCCTGCCGCTGGTAGAGGGCGTGGCGCCGCATTGCCCGAACGTGAAGGGCTGGG
TGATGATGTCCGACCGCGCCCACATGCCGGCCGAGTCGAAGGTCGACCTGATCTGCTACGAAGACCTGATCGACGCCCAG
GACGGAAACTACGAGTGGCCGCAGTTCGACGAAAACCAGGCGTCCAGCCTTTGCTACACGTCCGGTACCACGGGCAACCC
GAAGGGCGCGCTGTATTCGCATCGCTCGACGGTGTTGCACTCGTACGCCTCGGCACTGCCCGACGCGCTCGGATGCTCCG
CGCGTGACGTGATTCTGCCGGTCGTGCCGATGTTCCACGTCAATGCCTGGGGCCTGCCGTACTCGGTGCCGCTGGTCGGC
GCAAAGCTGGTGCTGCCCGGGCCGAAGCTTGACGGTGCTTCGCTGTTCGAACTGTTCGAGCAGGAGGGCGTGACCTTCTC
CGCCGGCGTGCCGACGGTCTGGCTGGGCCTGCTGCAATACGTCCAGGCCAACAAGCTGAAGTTCTCGACGTTCCGCCGCA
CCGTGATTGGCGGATCGGCGGCGCCGCCCGCAATGATCCGCGCGCTCGAAGCGCTGGACGTGGAAGTCATCCACGCATGG
GGCATGACCGAGATGTCGCCGCTGGGCACGTCCTGCAAGCTGCTGGCGCGCCATTTGGACCTGCCTGAGGAGGCGCGTCA
CAAGATCGTGGAGAAGCAGGGCCGCGTGATCTATGGCGTCGACATGAAGATCGTCGACGGTGAAGGCAAGGAACTGCCGT
GGGACGGCAAGGCCTTCGGCGACCTCTACGTGCGCGGCCCGTGGGTCATCGACCATTACTTCCGCAACGACAACAGCCCG
CTCGTGGATGGCTGGTTCCCCACCGGGGACGTGGCCACGATCGACGAAGAGGGCTACATGCAGATCACGGACCGCAGCAA
GGACGTGATCAAGTCCGGCGGCGAATGGATCTCGTCGATTGATGTCGAGAACGTCGCTGCCGCGCATCCGGCCGTGCACA
TGGCCGCCTGCATCTCCGCGTATCACCCGAAATGGGACGAGCGACCGCTGCTGGTGGTGGTCAAGCGCCCGAACATGGAG
ATCACGCGCGAGGAACTGCTCGGGTTCTTCGAGGGCAAGGTCGCCAAATGGTGGATTCCCGACGATGTGCTGTTCGTCAG
CGAGATTCCGCTCACGGCTACCGGCAAGATGCAGAAGCTCAAGTTGCGGGAGCAACTCAAGGACTACAAGCTGCCCACCG
CCTGA

Upstream 100 bases:

>100_bases
GGAGTATGGGAGCAATCGGCCAAAAATTAGCACGATTGTTCGTTTTTCAGACTGATCATTCAAGACTGGAGACAGTTTCA
TGGCTTTGATGGGACAAATG

Downstream 100 bases:

>100_bases
GGCCGATCGAGGTCGACCGCAGCGGGATATTCATCTGATCAGGTAGCAGCAGGCATTTGCGCGGGCTCAGGGAAAGGAGT
CCGCGCGCCGGTGACCGGCC

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSAPLLISSIIRHAARYYGSTEIVSRRTEGDLHRYTYRDCELRARKLAQALGALGVQQGERVGTLAWNGYRHLEIYYGVS
GMGAVCHTINPRLFPEQIAYIVNHADDQYVFFDSTFLPLVEGVAPHCPNVKGWVMMSDRAHMPAESKVDLICYEDLIDAQ
DGNYEWPQFDENQASSLCYTSGTTGNPKGALYSHRSTVLHSYASALPDALGCSARDVILPVVPMFHVNAWGLPYSVPLVG
AKLVLPGPKLDGASLFELFEQEGVTFSAGVPTVWLGLLQYVQANKLKFSTFRRTVIGGSAAPPAMIRALEALDVEVIHAW
GMTEMSPLGTSCKLLARHLDLPEEARHKIVEKQGRVIYGVDMKIVDGEGKELPWDGKAFGDLYVRGPWVIDHYFRNDNSP
LVDGWFPTGDVATIDEEGYMQITDRSKDVIKSGGEWISSIDVENVAAAHPAVHMAACISAYHPKWDERPLLVVVKRPNME
ITREELLGFFEGKVAKWWIPDDVLFVSEIPLTATGKMQKLKLREQLKDYKLPTA

Sequences:

>Translated_534_residues
MSAPLLISSIIRHAARYYGSTEIVSRRTEGDLHRYTYRDCELRARKLAQALGALGVQQGERVGTLAWNGYRHLEIYYGVS
GMGAVCHTINPRLFPEQIAYIVNHADDQYVFFDSTFLPLVEGVAPHCPNVKGWVMMSDRAHMPAESKVDLICYEDLIDAQ
DGNYEWPQFDENQASSLCYTSGTTGNPKGALYSHRSTVLHSYASALPDALGCSARDVILPVVPMFHVNAWGLPYSVPLVG
AKLVLPGPKLDGASLFELFEQEGVTFSAGVPTVWLGLLQYVQANKLKFSTFRRTVIGGSAAPPAMIRALEALDVEVIHAW
GMTEMSPLGTSCKLLARHLDLPEEARHKIVEKQGRVIYGVDMKIVDGEGKELPWDGKAFGDLYVRGPWVIDHYFRNDNSP
LVDGWFPTGDVATIDEEGYMQITDRSKDVIKSGGEWISSIDVENVAAAHPAVHMAACISAYHPKWDERPLLVVVKRPNME
ITREELLGFFEGKVAKWWIPDDVLFVSEIPLTATGKMQKLKLREQLKDYKLPTA
>Mature_533_residues
SAPLLISSIIRHAARYYGSTEIVSRRTEGDLHRYTYRDCELRARKLAQALGALGVQQGERVGTLAWNGYRHLEIYYGVSG
MGAVCHTINPRLFPEQIAYIVNHADDQYVFFDSTFLPLVEGVAPHCPNVKGWVMMSDRAHMPAESKVDLICYEDLIDAQD
GNYEWPQFDENQASSLCYTSGTTGNPKGALYSHRSTVLHSYASALPDALGCSARDVILPVVPMFHVNAWGLPYSVPLVGA
KLVLPGPKLDGASLFELFEQEGVTFSAGVPTVWLGLLQYVQANKLKFSTFRRTVIGGSAAPPAMIRALEALDVEVIHAWG
MTEMSPLGTSCKLLARHLDLPEEARHKIVEKQGRVIYGVDMKIVDGEGKELPWDGKAFGDLYVRGPWVIDHYFRNDNSPL
VDGWFPTGDVATIDEEGYMQITDRSKDVIKSGGEWISSIDVENVAAAHPAVHMAACISAYHPKWDERPLLVVVKRPNMEI
TREELLGFFEGKVAKWWIPDDVLFVSEIPLTATGKMQKLKLREQLKDYKLPTA

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=528, Percent_Identity=27.0833333333333, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI187761345, Length=522, Percent_Identity=25.4789272030651, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI187761343, Length=522, Percent_Identity=25.4789272030651, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=24.3614931237721, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=24.1779497098646, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=24.1779497098646, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI58082049, Length=521, Percent_Identity=23.9923224568138, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28416953, Length=582, Percent_Identity=24.0549828178694, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=23.495145631068, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI122937307, Length=520, Percent_Identity=23.6538461538462, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI13375727, Length=546, Percent_Identity=22.5274725274725, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=20.5078125, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI145693145, Length=506, Percent_Identity=27.4703557312253, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=22.972972972973, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1788107, Length=522, Percent_Identity=24.1379310344828, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786810, Length=361, Percent_Identity=28.808864265928, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789201, Length=481, Percent_Identity=23.9085239085239, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994703, Length=573, Percent_Identity=25.6544502617801, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=573, Percent_Identity=25.6544502617801, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=573, Percent_Identity=25.6544502617801, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=26.078431372549, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=25.9887005649718, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=367, Percent_Identity=29.4277929155313, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=494, Percent_Identity=23.6842105263158, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=525, Percent_Identity=25.3333333333333, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=23.5521235521236, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=23.4848484848485, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=383, Percent_Identity=26.1096605744125, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=500, Percent_Identity=21, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71996755, Length=508, Percent_Identity=21.6535433070866, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=396, Percent_Identity=28.5353535353535, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24581924, Length=560, Percent_Identity=25.3571428571429, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859661, Length=482, Percent_Identity=25.9336099585062, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21355181, Length=498, Percent_Identity=24.2971887550201, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648260, Length=507, Percent_Identity=23.6686390532544, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356441, Length=500, Percent_Identity=19.8, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356947, Length=500, Percent_Identity=21.4, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648257, Length=486, Percent_Identity=20.9876543209877, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653035, Length=494, Percent_Identity=20.0404858299595, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130357, Length=374, Percent_Identity=21.9251336898396, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 59438; Mature: 59306

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPLLISSIIRHAARYYGSTEIVSRRTEGDLHRYTYRDCELRARKLAQALGALGVQQGE
CCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
RVGTLAWNGYRHLEIYYGVSGMGAVCHTINPRLFPEQIAYIVNHADDQYVFFDSTFLPLV
EEEEEEECCEEEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHH
EGVAPHCPNVKGWVMMSDRAHMPAESKVDLICYEDLIDAQDGNYEWPQFDENQASSLCYT
HCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCEEEE
SGTTGNPKGALYSHRSTVLHSYASALPDALGCSARDVILPVVPMFHVNAWGLPYSVPLVG
CCCCCCCCCCEEHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCHHHEEECCCCCEECCCCC
AKLVLPGPKLDGASLFELFEQEGVTFSAGVPTVWLGLLQYVQANKLKFSTFRRTVIGGSA
EEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCEEHHHHHHHHCCCCC
APPAMIRALEALDVEVIHAWGMTEMSPLGTSCKLLARHLDLPEEARHKIVEKQGRVIYGV
CCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
DMKIVDGEGKELPWDGKAFGDLYVRGPWVIDHYFRNDNSPLVDGWFPTGDVATIDEEGYM
EEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCEEECCCCCCCEEEECCCCCE
QITDRSKDVIKSGGEWISSIDVENVAAAHPAVHMAACISAYHPKWDERPLLVVVKRPNME
EEECCCHHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
ITREELLGFFEGKVAKWWIPDDVLFVSEIPLTATGKMQKLKLREQLKDYKLPTA
CCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAPLLISSIIRHAARYYGSTEIVSRRTEGDLHRYTYRDCELRARKLAQALGALGVQQGE
CCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
RVGTLAWNGYRHLEIYYGVSGMGAVCHTINPRLFPEQIAYIVNHADDQYVFFDSTFLPLV
EEEEEEECCEEEEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHH
EGVAPHCPNVKGWVMMSDRAHMPAESKVDLICYEDLIDAQDGNYEWPQFDENQASSLCYT
HCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCEEEE
SGTTGNPKGALYSHRSTVLHSYASALPDALGCSARDVILPVVPMFHVNAWGLPYSVPLVG
CCCCCCCCCCEEHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCHHHEEECCCCCEECCCCC
AKLVLPGPKLDGASLFELFEQEGVTFSAGVPTVWLGLLQYVQANKLKFSTFRRTVIGGSA
EEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCEEHHHHHHHHCCCCC
APPAMIRALEALDVEVIHAWGMTEMSPLGTSCKLLARHLDLPEEARHKIVEKQGRVIYGV
CCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
DMKIVDGEGKELPWDGKAFGDLYVRGPWVIDHYFRNDNSPLVDGWFPTGDVATIDEEGYM
EEEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCEEECCCCCCCEEEECCCCCE
QITDRSKDVIKSGGEWISSIDVENVAAAHPAVHMAACISAYHPKWDERPLLVVVKRPNME
EEECCCHHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
ITREELLGFFEGKVAKWWIPDDVLFVSEIPLTATGKMQKLKLREQLKDYKLPTA
CCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]