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The map label for this gene is agrC [H]

Identifier: 94310686

GI number: 94310686

Start: 1894768

End: 1896234

Strand: Reverse

Name: agrC [H]

Synonym: Rmet_1748

Alternate gene names: 94310686

Gene position: 1896234-1894768 (Counterclockwise)

Preceding gene: 94310687

Following gene: 94310685

Centisome position: 48.27

GC content: 59.1

Gene sequence:

>1467_bases
ATGAGGCTAGCCATAAGTGCGGTTTGTATATGGCTAGCGGGGTGCTCTTTCGCACCGACTTACCACATTCCGGAGATGGA
TCTTCCCGCGAAGTATGCAGAAGCGAGCGATCCAGTCCTGAGTCAGATTCCCGCTGAGTGGTGGACGTCTTACCATGACG
ACGACCTTGATGCGTTAATTCGACGCATCGATGTGACAAATCAAACGCTCAAGAGATCGATAGCCCTTCTGCAAGATTCC
CGGGCCCAGGCCGATGCTGCACGCGCAGCGTACTTTCCGACAGCCGGCGCGAATGCCAGCGCTACCAGTAGTCATGTGTC
GGCAAACGTAGTTGGACGGTCGCTGGCCGGTAAGACAACGCCCGACCGGCTTATCGGATTCAGTGCGACCTGGGAGCCTG
ACCTTTTTGGGCGAATTGGCAATGCCGTCGATGCGGCAACGTCTCGAGTGCAAGCAAGTGCGGACGATGTCGCCGCAGTT
CGGCTGACCTTGCAGGCGGAAGTCGCTACGGATTACTTTGCGATCCGCTCGCTTGACCGCGAGGCATCGCTGCTAAGGCA
GACGGTGGACGCTTATTCTTCGGTGCTCTCGATGGTCACTAACAGATATAAGGGCGGAATTGCGGCTGAGAGCGATGTTG
CGCAGGCTCAAGCTCAGCGCGACAGTACTAAAGCTCAATTGGCTGACCTAACTCTTGCGCGAACCCAACTGCAACATGCG
CTCGCAACACTGGTGGGCGAGCCTGCCAGCACGTTTTCGATCGCACCACATGACGCCTATGGAACGCAACTTCCTGTAGT
ACCTGCCGCAGTTCCCAGCGAGTTATTGAAGCGACGTCCGGACATAGCTGCAGTAGAACGTCGTGTTTCGGCGGCCAATT
CCCAAATCGGCGTTGCTAAGGCCGCGTTCTTTCCGAGTCTCATGCTCAACCTGAGCGGAGGTGTCGAGAGCTCAGGCTTG
GCCGGACTCCTTACGGCCCCAGGTCGCTTCTGGGCGGTGGGGCCTGCCTTGGCTGCGACAATCTTCGACGGAGGGAAGCG
CCAAGCCGGCGTGGACAGTGCCCAGGCGCAATTCGATGCTGCTGCGGCCGACTACCGGCAGACCGTGCTAACGGCGGTCC
AAGACGTGGAGGACAGCTACGCGGCCACGAGACTCCTGAAGGAGGCTGCATCGAGTCAGGAAGCCGCAGTCCAGGCGTCG
CAACGCGCGCTCGCGCAGGCGACGGATCGCTACAAGAAAGGCGCGGCGGCATACCTCGACGTTAATGTGGTGGAGGCAAC
GACGCTCACTAACGAACGCACTCTCGAGAGTATTCATCGACGTCAGTTGGTCTCTAGCGTAGCGCTCTTTCGCTCGCTTG
GGGGAGGCTGGCAGGCTAAGGATGGGGCTGACGCTCAGAGTACCGCTGTCGACCCGTTGGCGACGTCAGAACCGGCGGCA
ACGGAAGGACTGCATTTAAAGCGTTAG

Upstream 100 bases:

>100_bases
GTGCCAAGGTTATCGTGAACCCGGGGGATGGGCTTGCCGGCGGCGCGCGTGTGAGAGTGTCTTCGCCGCAGACGGTCGCG
TCGCAAGGAGCTAAATCGTG

Downstream 100 bases:

>100_bases
TTGTCGCTGAACGCCGGTAACTCTTCCGTGCCGATCATGCTTTGAGGCCAAGGCTTGCCTGGCGTCGCCGGTTGATTTAA
GCTAAGGCGCATGACCCATT

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALIRRIDVTNQTLKRSIALLQDS
RAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTTPDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAV
RLTLQAEVATDYFAIRSLDREASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHA
LATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAKAAFFPSLMLNLSGGVESSGL
AGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDAAAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQAS
QRALAQATDRYKKGAAAYLDVNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAA
TEGLHLKR

Sequences:

>Translated_488_residues
MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALIRRIDVTNQTLKRSIALLQDS
RAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTTPDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAV
RLTLQAEVATDYFAIRSLDREASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHA
LATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAKAAFFPSLMLNLSGGVESSGL
AGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDAAAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQAS
QRALAQATDRYKKGAAAYLDVNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAA
TEGLHLKR
>Mature_488_residues
MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALIRRIDVTNQTLKRSIALLQDS
RAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTTPDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAV
RLTLQAEVATDYFAIRSLDREASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHA
LATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAKAAFFPSLMLNLSGGVESSGL
AGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDAAAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQAS
QRALAQATDRYKKGAAAYLDVNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAA
TEGLHLKR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=454, Percent_Identity=27.7533039647577, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI2367348, Length=466, Percent_Identity=27.4678111587983, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51541; Mature: 51541

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCHHHHCCCCCCCHHHHH
RRIDVTNQTLKRSIALLQDSRAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAVRLTLQAEVATDYFAIRSLDR
HHHHEECCCCCCCHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCH
EASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAK
HHHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
AAFFPSLMLNLSGGVESSGLAGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHH
AAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQASQRALAQATDRYKKGAAAYLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAA
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCC
TEGLHLKR
CCCCCCCC
>Mature Secondary Structure
MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCHHHHCCCCCCCHHHHH
RRIDVTNQTLKRSIALLQDSRAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAVRLTLQAEVATDYFAIRSLDR
HHHHEECCCCCCCHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCH
EASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAK
HHHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
AAFFPSLMLNLSGGVESSGLAGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHH
AAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQASQRALAQATDRYKKGAAAYLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAA
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCC
TEGLHLKR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]