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The map label for this gene is emrB [H]

Identifier: 94310566

GI number: 94310566

Start: 1752245

End: 1753843

Strand: Reverse

Name: emrB [H]

Synonym: Rmet_1624

Alternate gene names: 94310566

Gene position: 1753843-1752245 (Counterclockwise)

Preceding gene: 94310568

Following gene: 94310555

Centisome position: 44.65

GC content: 64.29

Gene sequence:

>1599_bases
ATGATTATAATAAGCACGCTTATTAATCTGCGCCAGCCCTTCCAGCCCCCCCCAATGTCCTCCGTGGTTTCCGGCGCGGC
ACAGCCAGCCGCCACCCCGCTCAACCGGCCGATGATCACCCTTTCGATCATGCTGGCCACGCTGATCCAGACGCTCGACA
GCACCATCGCCAACGTGGCCCTGCCGCACATGCAGGGTACGCTTTCGGCCTCGCAGGACGAAATCACATGGGTGCTGACT
TCCTACATCGTCGCCGCAGCCATCGCCACGCCGCTGACCGGCTGGCTGTCCGACCAGCTCGGCGTCAAGCGGCTGCTGGC
TATCTCGATCGGCGGATTTACCGTGGCTTCGGCGCTGTGCGGGATGGCGGACTCCCTCACCCAGATCGTGGCCGCGCGGC
TGCTGCAAGGGGTTTTTGGGGCGGCGCTGGTGCCGCTGTCCCAGTCGATCCTGCTCGATATCAATCCACCGTCGCGCCAG
GGGCAGGCCATGGCCGTGTGGGGCATGGGCGTGATGGTCGGGCCAATTCTCGGGCCCACACTGGGCGGCTGGCTGACCGA
CAGCTACAACTGGCGCTGGGTGTTCTTCATCAACGTGCCGATCGGCCTGTTCGCGTTGTTCGGCGTGCTGGCCTCGCTAC
CGAACCGCACCCCGGCGCGCAGCATGAGATTCGACGCGTTCGGCTTCGCCACGCTGAGTCTCGCTATCGGGGCGTTGCAG
GCCATGCTGGACCGCGGCGAACAACTTGACTGGTTCGGCTCGCTCGAAATCCGCGTCGAGGCGCTGATCTGCGTGATCGC
CTTCGCCTACTTCCTGATCCATACGGCCACAGCCGGCGAAAAGTCGTTCTTCCGTTCCGCGCTGCTCAAGGACCGTAATT
TCGTCACCGGGGCCTGTTTCATCTTCGTGATCGGCGCGGTCATGTATGCCACCCGCGCGCTGCTGCCGCCGATGCTGCAG
AACCTGATGGGCTATCCGGTGGCCACCACGGGGCTGGTTACCGCGCCAAGCGGGCTGGGCACGATGATCGCGATGATGCT
GGCGGGCCGGCTGCTGCGGCGCGTGGATGCGCGGCTGCTCCTGTTGGCCGGCTTCCTGATCTCCGCCTTCGCACTGTGGC
AGATGATGGGCTACACCGTGGTGCTTTCGATGTCGGATATCGTCGTACCGGGCATCATCCAGGGCTTCGGCCTGGGGCTC
GTGTTCGTACCGCTGTCGGCGCTGACGTTCTCCACACTGGAGCCGTCCCTGCGCGCCGATGGCACGGCCACATACAGCCT
GATGCGCAATATCGGCAGCAGCATCGGCATCTCGCTGATCCAGACCATGATGACCCGCAACGCGCAGGTGGCGCACAGCG
ACCTGGTCACGCATATCAACCCGTACAACCCGGCGCTGCAGTCGATGCTCACGCATGGCTCGCGGATCGACATCGCGGTG
CTGAACCAGACCATCAACCAGCAGGCAGCGATGATCGCCTATCTGAACGACTTCAAGGCGATGTTCATCGCCACGCTGGT
GGTGGTGCCGCTGATCCTGTTGATCCGCCCCGGCGCGAAGCACGCCGACGACAGCCTGGCGCACGCGGCGATGGATTGA

Upstream 100 bases:

>100_bases
TTCAAGATTTGTTTCCATCGCGCGATTATAGGGAGTCGGGGCGCTCGATCCGCTTTTCTGGCCGTCCGTAACTCGCGTGA
TCGCCCAAAAACGCCAGTCC

Downstream 100 bases:

>100_bases
TGAGGTCACCGGTGCGCCGACAGGCCGCAGCAACTACTCGAGCCGGATCCAGGTCGTCTTCAGGTCCTGGTACTTCTCCA
GCGCGTGCAACGACTTGTCG

Product: Drug resistance transporter EmrB/QacA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MIIISTLINLRQPFQPPPMSSVVSGAAQPAATPLNRPMITLSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLT
SYIVAAAIATPLTGWLSDQLGVKRLLAISIGGFTVASALCGMADSLTQIVAARLLQGVFGAALVPLSQSILLDINPPSRQ
GQAMAVWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVPIGLFALFGVLASLPNRTPARSMRFDAFGFATLSLAIGALQ
AMLDRGEQLDWFGSLEIRVEALICVIAFAYFLIHTATAGEKSFFRSALLKDRNFVTGACFIFVIGAVMYATRALLPPMLQ
NLMGYPVATTGLVTAPSGLGTMIAMMLAGRLLRRVDARLLLLAGFLISAFALWQMMGYTVVLSMSDIVVPGIIQGFGLGL
VFVPLSALTFSTLEPSLRADGTATYSLMRNIGSSIGISLIQTMMTRNAQVAHSDLVTHINPYNPALQSMLTHGSRIDIAV
LNQTINQQAAMIAYLNDFKAMFIATLVVVPLILLIRPGAKHADDSLAHAAMD

Sequences:

>Translated_532_residues
MIIISTLINLRQPFQPPPMSSVVSGAAQPAATPLNRPMITLSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLT
SYIVAAAIATPLTGWLSDQLGVKRLLAISIGGFTVASALCGMADSLTQIVAARLLQGVFGAALVPLSQSILLDINPPSRQ
GQAMAVWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVPIGLFALFGVLASLPNRTPARSMRFDAFGFATLSLAIGALQ
AMLDRGEQLDWFGSLEIRVEALICVIAFAYFLIHTATAGEKSFFRSALLKDRNFVTGACFIFVIGAVMYATRALLPPMLQ
NLMGYPVATTGLVTAPSGLGTMIAMMLAGRLLRRVDARLLLLAGFLISAFALWQMMGYTVVLSMSDIVVPGIIQGFGLGL
VFVPLSALTFSTLEPSLRADGTATYSLMRNIGSSIGISLIQTMMTRNAQVAHSDLVTHINPYNPALQSMLTHGSRIDIAV
LNQTINQQAAMIAYLNDFKAMFIATLVVVPLILLIRPGAKHADDSLAHAAMD
>Mature_532_residues
MIIISTLINLRQPFQPPPMSSVVSGAAQPAATPLNRPMITLSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLT
SYIVAAAIATPLTGWLSDQLGVKRLLAISIGGFTVASALCGMADSLTQIVAARLLQGVFGAALVPLSQSILLDINPPSRQ
GQAMAVWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVPIGLFALFGVLASLPNRTPARSMRFDAFGFATLSLAIGALQ
AMLDRGEQLDWFGSLEIRVEALICVIAFAYFLIHTATAGEKSFFRSALLKDRNFVTGACFIFVIGAVMYATRALLPPMLQ
NLMGYPVATTGLVTAPSGLGTMIAMMLAGRLLRRVDARLLLLAGFLISAFALWQMMGYTVVLSMSDIVVPGIIQGFGLGL
VFVPLSALTFSTLEPSLRADGTATYSLMRNIGSSIGISLIQTMMTRNAQVAHSDLVTHINPYNPALQSMLTHGSRIDIAV
LNQTINQQAAMIAYLNDFKAMFIATLVVVPLILLIRPGAKHADDSLAHAAMD

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=437, Percent_Identity=37.9862700228833, Blast_Score=301, Evalue=9e-83,
Organism=Escherichia coli, GI1788710, Length=492, Percent_Identity=34.9593495934959, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1790195, Length=405, Percent_Identity=28.8888888888889, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=30.8080808080808, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=29.1469194312796, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87082320, Length=175, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=30.8270676691729, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=391, Percent_Identity=25.5754475703325, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56871; Mature: 56871

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIISTLINLRQPFQPPPMSSVVSGAAQPAATPLNRPMITLSIMLATLIQTLDSTIANVA
CEEHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPHMQGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDQLGVKRLLAISIGGFTVASALC
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GMADSLTQIVAARLLQGVFGAALVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCC
LGGWLTDSYNWRWVFFINVPIGLFALFGVLASLPNRTPARSMRFDAFGFATLSLAIGALQ
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHH
AMLDRGEQLDWFGSLEIRVEALICVIAFAYFLIHTATAGEKSFFRSALLKDRNFVTGACF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
IFVIGAVMYATRALLPPMLQNLMGYPVATTGLVTAPSGLGTMIAMMLAGRLLRRVDARLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
LLAGFLISAFALWQMMGYTVVLSMSDIVVPGIIQGFGLGLVFVPLSALTFSTLEPSLRAD
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GTATYSLMRNIGSSIGISLIQTMMTRNAQVAHSDLVTHINPYNPALQSMLTHGSRIDIAV
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHH
LNQTINQQAAMIAYLNDFKAMFIATLVVVPLILLIRPGAKHADDSLAHAAMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIIISTLINLRQPFQPPPMSSVVSGAAQPAATPLNRPMITLSIMLATLIQTLDSTIANVA
CEEHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPHMQGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDQLGVKRLLAISIGGFTVASALC
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GMADSLTQIVAARLLQGVFGAALVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCC
LGGWLTDSYNWRWVFFINVPIGLFALFGVLASLPNRTPARSMRFDAFGFATLSLAIGALQ
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHH
AMLDRGEQLDWFGSLEIRVEALICVIAFAYFLIHTATAGEKSFFRSALLKDRNFVTGACF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
IFVIGAVMYATRALLPPMLQNLMGYPVATTGLVTAPSGLGTMIAMMLAGRLLRRVDARLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
LLAGFLISAFALWQMMGYTVVLSMSDIVVPGIIQGFGLGLVFVPLSALTFSTLEPSLRAD
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GTATYSLMRNIGSSIGISLIQTMMTRNAQVAHSDLVTHINPYNPALQSMLTHGSRIDIAV
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHH
LNQTINQQAAMIAYLNDFKAMFIATLVVVPLILLIRPGAKHADDSLAHAAMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]