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The map label for this gene is mii [H]

Identifier: 94310526

GI number: 94310526

Start: 1704945

End: 1706138

Strand: Reverse

Name: mii [H]

Synonym: Rmet_1584

Alternate gene names: 94310526

Gene position: 1706138-1704945 (Counterclockwise)

Preceding gene: 94310527

Following gene: 94310525

Centisome position: 43.43

GC content: 66.25

Gene sequence:

>1194_bases
ATGGCACATGTACCCCAAATCAGGATTCCCGCCACCTACATGCGTGGCGGTACCAGCAAGGGCGTGTTCTTCCGGGTGCA
GGACCTGCCAGAGGCTGCGCAGGTCCCGGGCAAGGCGCGCGACGCGCTGCTGATGCGCGTGATCGGCAGCCCCGATCCGT
ACGGCAAGCAGATCGACGGCATGGGCGCGGCCACCTCGAGTACCAGCAAGACCGTGCTGATCTCGAAGAGCACCCGCCCG
GACCACGACGTCGACTATTTGTTCGGGCAGGTGTCGATCGACCAGCCGTTCGTAGACTGGAGCGGCAACTGCGGCAACCT
GTCGGCGGCGGTCGGTCCGTACGCGATCAGCAACGGCTTCATCGACGCGGCGCGCATTCCCGAGAACGGCACGGTGACGG
TCCGGATCTGGCAAGCGAACATCGGCAAGACCATCATCTCGCACGTGCCGATCACCAACGGCGCGGTGCAGGAAACGGGC
GACTTCGAGCTCGACGGCGTCACGTTCCCCGCGGCCGAAGTCCAGCTCGAGTTCATGGATCCGGCCGCCGAGGAAGGCGA
TGACGGTGGCGGCGCGATGTTCCCGACCGGTAACCTCGTCGATGACCTCGAAGTGCCCGGCGTCGGCACGCTCAAGGCCA
CGATGATCAATGCCGGCATTCCGACGATCTTCGTCAATGCGGAGGCCATCGGCTACACCGGCACGGAACTGCAGGAAGCC
ATCAACGGCGACCCGAAGGCGCTGGCGATGTTCGAGACCATCCGTGCCTATGGCGCCAAGCGCATGGGCCTGATCCGCGA
CATCGACGAAGCCGCGCGTCGCCAGCACACGCCGAAGGTGGCGTTCGTGGCGAAGCCGGCGACGTACACGGCATCGAGCG
GCAAGACCGTCAATGCCGGCGACGTGGACCTGCTGGTGCGTGCGCTGTCGATGGGCAAGCTGCACCATGCGATGATGGGC
ACCGCTGCGGTGGCCATCGGCACGGCGGCTGCAGTACCGGGCACGCTGGTGAACCTGGCGGCCGGCGGCGGCGAACGCGA
GGCAGTGCGTTTCGGCCACCCATCCGGCACGCTGCGTGTGGGCGCGCAGGCCGTCCAGTCCGACGGTGAATGGACCGTGA
CGAAGGCCATCATGAGCCGCAGTGCACGCGTGTTGATGGAAGGCTGGGTTCGCATACCGGGCGACGCGTTCTAA

Upstream 100 bases:

>100_bases
ACGAGGAAGTTTCGATCTACGAAGCAGGCGGCGTGCTGCAGCGCTTTGCGCAGGACTTCCTCGAGTCCTCGCGCGCGGCG
GCCTGACGGGAGACTGACGA

Downstream 100 bases:

>100_bases
GCGCGTACAGACGGAAGGTCCTCGCTGCTTGAACGAAGCGGCATCCTCTCTGGCAGAGGATGCCGCTTTGTCTTTTCAGG
CATACGTATGCGGCTGTGAC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVLISKSTRP
DHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGFIDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEA
INGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAGDVDLLVRALSMGKLHHAMMG
TAAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF

Sequences:

>Translated_397_residues
MAHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVLISKSTRP
DHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGFIDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEA
INGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAGDVDLLVRALSMGKLHHAMMG
TAAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF
>Mature_396_residues
AHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVLISKSTRPD
HDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGFIDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETGD
FELDGVTFPAAEVQLEFMDPAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEAI
NGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAGDVDLLVRALSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=386, Percent_Identity=33.9378238341969, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41638; Mature: 41507

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDG
CCCCCCEECCHHHHCCCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCC
MGAATSSTSKTVLISKSTRPDHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGF
CCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCC
IDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
EEEEECCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCCHHHHHH
INGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAG
HCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCEECCC
DVDLLVRALSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEE
GAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF
CHHHHHCCCCEEHHHHHHHHHHHEEECCCEECCCCCC
>Mature Secondary Structure 
AHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDG
CCCCCEECCHHHHCCCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCC
MGAATSSTSKTVLISKSTRPDHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGF
CCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCC
IDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
EEEEECCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCCHHHHHH
INGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAG
HCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCEECCC
DVDLLVRALSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEE
GAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF
CHHHHHCCCCEEHHHHHHHHHHHEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA