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The map label for this gene is ansB [H]

Identifier: 94310525

GI number: 94310525

Start: 1703543

End: 1704670

Strand: Reverse

Name: ansB [H]

Synonym: Rmet_1583

Alternate gene names: 94310525

Gene position: 1704670-1703543 (Counterclockwise)

Preceding gene: 94310526

Following gene: 94310523

Centisome position: 43.4

GC content: 64.45

Gene sequence:

>1128_bases
ATGCAACGCACTGCCCGTCTCAAACCCCTGGCCGCCCTGCTGCTTTCGGCCAGCCTGCTCACTGGCGTGGCTCACGCCCA
GCTCAGCCCCGCACCGGCCGCCAGCGCCAATGCGCCGGCAGGCACCACCGCCGAAGCACGCAAGGCCAACATCGTCATCA
TCGGCACTGGCGGCACCATCGCCGGCGCGGGTGCTGCCGCCACCAATACCGCGGCCTATCAGTCCGCCGTGGTGCCGGTC
GACAAGATCATCGCTTCCGTGCCGGAGATCTCGAAGATCGCCAACGTCAAGGGTGAACAGGTCTTCCAGATCGGCTCCGA
GAGCTTCAACAACGAGCGCCTGATCAAGCTGGCCAAGCGCGTCTCCGAACTGCTCAAGCAGCCCGACGTCGACGGCATCG
TGATCACGCACGGCACTGACACGATCGAGGAAACCGCATACTTCCTGAACCTGACGCTCAAGAGCGACAAGCCGGTGGTG
GTGGTCGGCTCGATGCGCCCGGGCACCGCACTGGGCGCCGATGGCGCGCTGAACCTGTACGACGCCGTGCTGGTGGCGTC
GAACCCCGCCTCGAAGGGCAAGGGCACGCTGGTGGTGCTCAATGACGAGATCCAGACCGGCCGTGACGTGACGAAGACCA
ACACGTTCAAGGTCGAGACGTTCCGCTCGCCGTTTGGCCCGCTCGGCTACGTGGTGGAAGGCCGCACGCTGTATTTCCGC
CTGCCGGCCCGGCCGCACACGACGCAGACGGAGTTCGACATCGACAAGATCGACAAGCTGCCTGAAGTGGCCGTGGTCTA
CGCGTACGGCAACATGAACCCTGCCGCGATCGATGCCGCTGTGAAGAGTGGCGCCAAGGCCATCATCTTCGACGCCACCG
GCAATGGTAGCGTCGGCGACTACATGGTCGAACCGCTCAAGGCCGCCCGTGCCAAGGGCGTGTTCATTGTGCGTGCCTCG
CGCACGGGCAGCGGCGTCGTGATCCGTAACGGCGAACAGCCCGACGACAAGTACGACTGGATCGTCACCGACGACCAGAT
CGCGCAGAAGGCTCGCATCCTGATGGCGCTGGCACTGACCAAGACCAACGACCCGAAGGCACTGCAAGAGATCTTCTGGA
AGTACTGA

Upstream 100 bases:

>100_bases
GAAAACCCTGCGGCGCTTGACGAGTGGAATCCATGAATCGAAAACCTGCGGCTCTCGCGCTTCCACAAGACGCGCGCCGT
TCGCCGATATGAGGAGAATC

Downstream 100 bases:

>100_bases
TCCTGGTGAACCTGGTGTGAACTAGATCAGGGCCGTGCTCATCAATGCCTTGGCAACGAGCACGGCCTCGGCATCGCCCT
CCGCATAGACCGAGACCTCG

Product: periplasmic L-asparaginase II

Products: NA

Alternate protein names: L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTIAGAGAAATNTAAYQSAVVPV
DKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVV
VVGSMRPGTALGADGALNLYDAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFR
LPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGDYMVEPLKAARAKGVFIVRAS
RTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALTKTNDPKALQEIFWKY

Sequences:

>Translated_375_residues
MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTIAGAGAAATNTAAYQSAVVPV
DKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVV
VVGSMRPGTALGADGALNLYDAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFR
LPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGDYMVEPLKAARAKGVFIVRAS
RTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALTKTNDPKALQEIFWKY
>Mature_375_residues
MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTIAGAGAAATNTAAYQSAVVPV
DKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVV
VVGSMRPGTALGADGALNLYDAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFR
LPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGDYMVEPLKAARAKGVFIVRAS
RTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALTKTNDPKALQEIFWKY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=304, Percent_Identity=46.3815789473684, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788065, Length=209, Percent_Identity=30.622009569378, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=357, Percent_Identity=38.9355742296919, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323187, Length=357, Percent_Identity=38.9355742296919, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323186, Length=357, Percent_Identity=38.9355742296919, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323184, Length=357, Percent_Identity=38.9355742296919, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320527, Length=339, Percent_Identity=35.693215339233, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24645530, Length=207, Percent_Identity=28.0193236714976, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.38 [H]

Molecular weight: Translated: 39692; Mature: 39692

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCE
AGAGAAATNTAAYQSAVVPVDKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKR
ECCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
VSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLY
HHHHHHCCCCCEEEEECCCCCHHCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCE
DAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFR
EEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCEEEEE
LPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGD
CCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCH
YMVEPLKAARAKGVFIVRASRTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALT
HHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHEEEEE
KTNDPKALQEIFWKY
CCCCHHHHHHHHHCC
>Mature Secondary Structure
MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCE
AGAGAAATNTAAYQSAVVPVDKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKR
ECCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
VSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLY
HHHHHHCCCCCEEEEECCCCCHHCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCE
DAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFR
EEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCEEEEE
LPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGD
CCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCH
YMVEPLKAARAKGVFIVRASRTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALT
HHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHEEEEE
KTNDPKALQEIFWKY
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA