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The map label for this gene is hypB2 [H]

Identifier: 94310478

GI number: 94310478

Start: 1655936

End: 1656916

Strand: Direct

Name: hypB2 [H]

Synonym: Rmet_1536

Alternate gene names: 94310478

Gene position: 1655936-1656916 (Clockwise)

Preceding gene: 94310477

Following gene: 94310479

Centisome position: 42.16

GC content: 66.16

Gene sequence:

>981_bases
ATGTGTACCATCTGCGGCTGCGGCGCCGGCGAAACCCGCATCGAAGGCCAGGAACTGGAGCATACGCACGAGCACGTGCA
TGCCGACGGCACCGTCCATGCCCATGCCCACCCTCCTCACGAACAGGATCACCATTACGCGGATCATGACCACGACCACG
CTCATGCCCACCGCGCCGTGCGCGGCACCGACCATCTGCACTACGGTCACGGACCCGCTGGTGCCCATGCCCCAGGCATG
AGCCAGGCGCGGATGGTGAAAATCGAGCAGGACATCCTCGGCAAGAACAACGCCTACGCGGCGCAGAACCGCCGCTGGTT
CGATGAGCACGGCGTGTTCGCGCTGAACTTCGTCTCCAGCCCCGGCTCCGGCAAGACCACGCTGCTGGTGCGCACCATCG
AGGCGCTGCAATCCACCTGCGGGCTGGCCGTCATCGAGGGCGACCAGCAGACCTCCTTCGACGCCGAGCGCATCCGCGCC
ACCGGCGTGCGCGCCCTGCAGATCAACACCGGCAAGGGCTGCCACCTGGACGCCTACATGGTAGGTCACGCCCTGGAGCA
GCTGCGGCCGCAGGACGAGAGCGTGCTGCTGATCGAGAACGTGGGCAACCTGGTGTGTCCTTCCGCCTTCGACCTGGGCG
AAGCCCACAAGGTGGTGATCCTCTCCGTCACCGAGGGGGAAGACAAGCCCCTCAAGTATCCGGACATGTTCCGGGCTGCC
AGCCTGATGTTGCTCAACAAGTGCGATTTGCTGCCGCACCTGTCCTTCGACGTGGAGCGGACCATCGAGTATGCCCGGCG
CGTGAATCCCGCGCTGCACGTGATCCGCACCTCGTCCACCACCGGTGAAGGCTTCGCCGAGTGGCTGGCGTGGATCACTG
AAGGACTGGTCGGCCAGCGCGCCAAGCGCAGCCAGAACGTGGACCTGCTGCGCGCCCGCGTCGCCGAGCTGGAAGCGGAG
CTGGCGGTGCTCAAGCGCTGA

Upstream 100 bases:

>100_bases
CGGCAGCAGCCAGGTGCAGCCCACTGGCGGCACGGAAATGCGCGTCAGGGATCTGGAAGTGGCGTAACCTCACGTCAGGA
CAAGGAGACAGAATCGAATC

Downstream 100 bases:

>100_bases
TCGCCGAGCCCGCATGCTGATGCCGCGAACCCAGCGCCAGCCCCGGCCCGTCCGCATCCGCATCCGCGTGCGCGGCGTGG
TGCAGGGCGTGGGCTTCCGG

Product: GTP hydrolase involved in nickel liganding into hydrogenases

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAVRGTDHLHYGHGPAGAHAPGM
SQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSSPGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRA
TGVRALQINTGKGCHLDAYMVGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAA
SLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQRAKRSQNVDLLRARVAELEAE
LAVLKR

Sequences:

>Translated_326_residues
MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAVRGTDHLHYGHGPAGAHAPGM
SQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSSPGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRA
TGVRALQINTGKGCHLDAYMVGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAA
SLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQRAKRSQNVDLLRARVAELEAE
LAVLKR
>Mature_326_residues
MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAVRGTDHLHYGHGPAGAHAPGM
SQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSSPGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRA
TGVRALQINTGKGCHLDAYMVGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAA
SLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQRAKRSQNVDLLRARVAELEAE
LAVLKR

Specific function: Could be involved in nickel binding and accumulation [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=292, Percent_Identity=54.7945205479452, Blast_Score=311, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 35918; Mature: 35918

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAV
CCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
RGTDHLHYGHGPAGAHAPGMSQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSS
CCCCCEECCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCHHHCCEEEEEEECC
PGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRATGVRALQINTGKGCHLDAYM
CCCCCEEEHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCEEEEEEECCCCCEEHHHH
VGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAA
HHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
SLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQR
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AKRSQNVDLLRARVAELEAELAVLKR
HHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAV
CCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
RGTDHLHYGHGPAGAHAPGMSQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSS
CCCCCEECCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCHHHCCEEEEEEECC
PGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRATGVRALQINTGKGCHLDAYM
CCCCCEEEHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCEEEEEEECCCCCEEHHHH
VGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAA
HHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
SLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQR
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AKRSQNVDLLRARVAELEAELAVLKR
HHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486288 [H]