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The map label for this gene is hoxH [H]

Identifier: 94310467

GI number: 94310467

Start: 1647176

End: 1648642

Strand: Direct

Name: hoxH [H]

Synonym: Rmet_1525

Alternate gene names: 94310467

Gene position: 1647176-1648642 (Clockwise)

Preceding gene: 94310466

Following gene: 94310468

Centisome position: 41.93

GC content: 60.87

Gene sequence:

>1467_bases
ATGAGCAGAAAACTGGTTATCGATCCCGTGACCCGTATCGAGGGTCACGGCAAGGTCACGGTGCACCTGGATGACGACAA
TAACGTCATCGACGCCAAGCTGCACGTCGTGGAATTCCGGGGTTTCGAAAAGTTCATCCAGGGCCATCCCTACTGGGAGG
CGCCGATGTTTCTGCAGCGTATCTGCGGCATCTGCTTTGTCAGCCACCACCTGTGCGGGGCCAAGGCGCTGGACGACATG
GTCGGTGTCGGCCTGAAATCCGGCATCGATGTCACACCGGCCGCGGAGAAAATGCGCCGCCTCGGCCACTACGCGCAGAT
GCTCCAATCCCATACGACGGCCTATTTCTATCTGATCGTGCCGGAGATGCTGTTCGGGATGGACGCGCCGCCTGAGCAGC
GCAACGTGCTCGGCCTGATTGAGGCCGATCCCGAGCTGGTGAAAAGAGTCGTGATGCTGCGCAAATGGGGCCAGGAAGTC
ATCAAGGTGGTGTTCGGCAAAAAAATGCACGGCATCAACTCGGTGCCCGGTGGCGTCAACAAGAATCTGAGCATTGCCGA
GCGCGACCGTTTGCTGAACGGGGAAGAAGGCCTTCTGGCGATGGATCAGGTCATCGACTTCGCACAGGATGGACTGCGCC
TGTTCTACGACTTCCATGAAAAGCACCGGGCGCAGGTGGACAGTTTCGCCGACGTACCCGCACTCAACATGTCCCTGGTC
GATGCCGACGGCAATGTGGACTACTACCACGGCAAGCTGCGTATCGTCGACGACGACAAGAACATCGTCCGCGAACTCGA
TTATCACGACTACCTGGATCACTTCAGCGAAGCGGTCGAGGAGTGGAGCTACATGAAGTTCCCTTACCTCAAAGACCTGG
GAAGGGAAAAGGGTTCGGTGCGCGTCGGGCCGCTGGGCCGCATGAACGTGACGAAGACGCTCTCGACGCCGCTCGCCCAG
GAGGCGCTGGAACGCTTCCATGCCTACACGAAGGGGCGGGCGAACAACATGACCCTGCACACCAACTGGGCACGGGCCAT
AGAAATCCTCCACGCCGCGGAGGTCATCAGGGAATTGCTGCACGACCCGGACCTGCAGAAGGATCAGCTGGTGTTGACGC
CGCCCGCCGGCGCGTGGACCGGCGAAGGGGTCGGTGTGGTCGAAGCGCCACGTGGAACCTTGCTCCACCATTACCGTGCC
GACGAGCGCGGCAATATCACCTTCGCCAACCTGGTCGTCGCCACCACTCAAAACAACCAGGTCATGAATCGCACGGTGCG
CAGCGTCGCCGAGGACTACCTGGGCGGGCACGGCGAGATTACCGAGGGCATGATGAACGCCATCGAGGTGGGCATACGCG
CATACGATCCTTGCCTGAGCTGCGCCACCCACGCCCTGGGGCAGATGCCGCTGGTGGTTTCGGTCTATGACGCGGCGGGG
GGGCTGATCGATGAACGCACCCGCTGA

Upstream 100 bases:

>100_bases
GAGACGCCATCTTCAAGGTCCTGGACGATCTGGTCAACGGGCGTCCATTCGATTTGCCGAGCTCGATCAATCGCTACGAT
TGATAAACGGAGGTAATGTC

Downstream 100 bases:

>100_bases
GTTTCCCCTCGTGACCCTTGAGGATTTCGACGATCCGTCGAGCCTGATTTATGGCATCGGCAACGTCGGTCGGCAGGACG
ACGGACTGGGCTGGGCCTTG

Product: NAD-reducing hydrogenase hoxS subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSRKLVIDPVTRIEGHGKVTVHLDDDNNVIDAKLHVVEFRGFEKFIQGHPYWEAPMFLQRICGICFVSHHLCGAKALDDM
VGVGLKSGIDVTPAAEKMRRLGHYAQMLQSHTTAYFYLIVPEMLFGMDAPPEQRNVLGLIEADPELVKRVVMLRKWGQEV
IKVVFGKKMHGINSVPGGVNKNLSIAERDRLLNGEEGLLAMDQVIDFAQDGLRLFYDFHEKHRAQVDSFADVPALNMSLV
DADGNVDYYHGKLRIVDDDKNIVRELDYHDYLDHFSEAVEEWSYMKFPYLKDLGREKGSVRVGPLGRMNVTKTLSTPLAQ
EALERFHAYTKGRANNMTLHTNWARAIEILHAAEVIRELLHDPDLQKDQLVLTPPAGAWTGEGVGVVEAPRGTLLHHYRA
DERGNITFANLVVATTQNNQVMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHALGQMPLVVSVYDAAG
GLIDERTR

Sequences:

>Translated_488_residues
MSRKLVIDPVTRIEGHGKVTVHLDDDNNVIDAKLHVVEFRGFEKFIQGHPYWEAPMFLQRICGICFVSHHLCGAKALDDM
VGVGLKSGIDVTPAAEKMRRLGHYAQMLQSHTTAYFYLIVPEMLFGMDAPPEQRNVLGLIEADPELVKRVVMLRKWGQEV
IKVVFGKKMHGINSVPGGVNKNLSIAERDRLLNGEEGLLAMDQVIDFAQDGLRLFYDFHEKHRAQVDSFADVPALNMSLV
DADGNVDYYHGKLRIVDDDKNIVRELDYHDYLDHFSEAVEEWSYMKFPYLKDLGREKGSVRVGPLGRMNVTKTLSTPLAQ
EALERFHAYTKGRANNMTLHTNWARAIEILHAAEVIRELLHDPDLQKDQLVLTPPAGAWTGEGVGVVEAPRGTLLHHYRA
DERGNITFANLVVATTQNNQVMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHALGQMPLVVSVYDAAG
GLIDERTR
>Mature_487_residues
SRKLVIDPVTRIEGHGKVTVHLDDDNNVIDAKLHVVEFRGFEKFIQGHPYWEAPMFLQRICGICFVSHHLCGAKALDDMV
GVGLKSGIDVTPAAEKMRRLGHYAQMLQSHTTAYFYLIVPEMLFGMDAPPEQRNVLGLIEADPELVKRVVMLRKWGQEVI
KVVFGKKMHGINSVPGGVNKNLSIAERDRLLNGEEGLLAMDQVIDFAQDGLRLFYDFHEKHRAQVDSFADVPALNMSLVD
ADGNVDYYHGKLRIVDDDKNIVRELDYHDYLDHFSEAVEEWSYMKFPYLKDLGREKGSVRVGPLGRMNVTKTLSTPLAQE
ALERFHAYTKGRANNMTLHTNWARAIEILHAAEVIRELLHDPDLQKDQLVLTPPAGAWTGEGVGVVEAPRGTLLHHYRAD
ERGNITFANLVVATTQNNQVMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHALGQMPLVVSVYDAAGG
LIDERTR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=155, Percent_Identity=31.6129032258064, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.1.2 [H]

Molecular weight: Translated: 54659; Mature: 54528

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKLVIDPVTRIEGHGKVTVHLDDDNNVIDAKLHVVEFRGFEKFIQGHPYWEAPMFLQR
CCCCEEECHHHHCCCCCEEEEEECCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHHH
ICGICFVSHHLCGAKALDDMVGVGLKSGIDVTPAAEKMRRLGHYAQMLQSHTTAYFYLIV
HHHHHHHHHHHCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEH
PEMLFGMDAPPEQRNVLGLIEADPELVKRVVMLRKWGQEVIKVVFGKKMHGINSVPGGVN
HHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KNLSIAERDRLLNGEEGLLAMDQVIDFAQDGLRLFYDFHEKHRAQVDSFADVPALNMSLV
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DADGNVDYYHGKLRIVDDDKNIVRELDYHDYLDHFSEAVEEWSYMKFPYLKDLGREKGSV
ECCCCEEEEEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCE
RVGPLGRMNVTKTLSTPLAQEALERFHAYTKGRANNMTLHTNWARAIEILHAAEVIRELL
EECCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
HDPDLQKDQLVLTPPAGAWTGEGVGVVEAPRGTLLHHYRADERGNITFANLVVATTQNNQ
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEHHCCCCCCCCEEEEEEEEEECCCCH
VMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHALGQMPLVVSVYDAAG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHC
GLIDERTR
CCCCCCCC
>Mature Secondary Structure 
SRKLVIDPVTRIEGHGKVTVHLDDDNNVIDAKLHVVEFRGFEKFIQGHPYWEAPMFLQR
CCCEEECHHHHCCCCCEEEEEECCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHHH
ICGICFVSHHLCGAKALDDMVGVGLKSGIDVTPAAEKMRRLGHYAQMLQSHTTAYFYLIV
HHHHHHHHHHHCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEH
PEMLFGMDAPPEQRNVLGLIEADPELVKRVVMLRKWGQEVIKVVFGKKMHGINSVPGGVN
HHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KNLSIAERDRLLNGEEGLLAMDQVIDFAQDGLRLFYDFHEKHRAQVDSFADVPALNMSLV
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DADGNVDYYHGKLRIVDDDKNIVRELDYHDYLDHFSEAVEEWSYMKFPYLKDLGREKGSV
ECCCCEEEEEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCE
RVGPLGRMNVTKTLSTPLAQEALERFHAYTKGRANNMTLHTNWARAIEILHAAEVIRELL
EECCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
HDPDLQKDQLVLTPPAGAWTGEGVGVVEAPRGTLLHHYRADERGNITFANLVVATTQNNQ
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEHHCCCCCCCCEEEEEEEEEECCCCH
VMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHALGQMPLVVSVYDAAG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHC
GLIDERTR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2188945; 12948488; 2496982 [H]