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The map label for this gene is pgk1 [H]

Identifier: 94310458

GI number: 94310458

Start: 1637843

End: 1639033

Strand: Direct

Name: pgk1 [H]

Synonym: Rmet_1516

Alternate gene names: 94310458

Gene position: 1637843-1639033 (Clockwise)

Preceding gene: 94310457

Following gene: 94310459

Centisome position: 41.7

GC content: 70.03

Gene sequence:

>1191_bases
GTGAACGTGTTGCGCTTCGCCGACCTCGTCGCCCAGGGCCGCGTGTGCGGCCAGCGCGTCTTCATCCGCGCCGACCTCAA
CGTGCCGCAGGACGATGCGGGGAACATCACCGAAGACACGCGCATCCGCGCCTCGGTGCTCTGCATCCGCATGGCGCTGG
ACGCCGGCGCGGCGGTCATGGTCACCTCGCACCTCGGCCGCCCGACCGAAGGCGAGTTCAAGCCCGAGGACTCGCTCGCG
CCGGTGGCCGCACGGCTCGCCGCGCTGCTCGGGCGGCCGGTGCCGCTGAAGTCGAACTGGGTGGGTGGCGTGGACGTGCA
GCCCGGCGAGGTGGTGTTGCTCGAGAACTGTCGCCTCAACAAGGGCGAGAAGAAGAACAGCGAGGCGCTGGCGCGCCAGA
TGGCGGCGCTGACCGACATCTTCGTGCACGACGCCTTCGGCACCGCGCACCGCGCCGAAGCCTCCACCTACGGCATCGCG
CAGTTCGCGCCGGTGGCTTGTGCCGGCCCTCTGCTCGCGGCGGAAATCGACGCGATCGGCAAGGCGCTGGCTGCCCCGAA
GCGCCCGCTGGTCGCCATCGTCGCCGGCAGCAAGGTCAGCACCAAGCTCACGATCCTGCAAAGCCTGGCCAAGAACGTCG
ATGGCCTGATCGTCGGCGGCGGCATCGCCAACACCTTCCTGCTCGCGCAGGGCCTGCCGATCGGCAAGAGCCTCGCCGAG
CCGGACTTGGTGGATGAGGCTAGGGCGGTGATCGACGCGATGAAGGCCCGCGGCGCCGAGGTGCCGATCCCGGTGGACGT
GGTCTGCGCCAAGGCGTTCAAGGCCGACGCGCCGGCCACCGTCAAGGCCGTGGCCGAGGTGGACGACGACGACCTGATCC
TCGACATCGGCCCGCAGACCGCCACGCTGCTCGCGCACAAGCTCGCGGCCGCCGGCACCATCGTCTGGAACGGACCGGTG
GGCGTATTCGAGTTCGACGCGTTCGCCAACGGCACCAAGACCATCGCGCGCGCGATCGCGGCCAGCGACGCCTTCAGCAT
CGCCGGCGGCGGCGACACGCTCGCGGCGATCGCCAAGTATGGCATCGACAAGGACGTGGGCTACATCTCCACCGGCGGTG
GCGCCTTCCTTGAGGTGCTCGAGGGCAAGACGCTGCCGGCCTTCGAGATCCTGGCCAGGCGCGCGCAATGA

Upstream 100 bases:

>100_bases
TGCTCGACTCGACCTTCGTCAAGGTGGTGGCCTGGTACGACAACGAATGGGGCTACTCGAGCAAGGTGCTCGAGATGGTG
CGCGTGATGGGAGCGTCGCG

Downstream 100 bases:

>100_bases
CCACCGGAACGGCGGCAAGACCCAAGCTCGAGGCGCTGCTGTGGGACGTCGACGGCACCCTCGCCGAGACCGAGCGCGAC
GGGCATCGGGTCGCGTTCAA

Product: phosphoglycerate kinase (Pgk)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVMVTSHLGRPTEGEFKPEDSLA
PVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLNKGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIA
QFAPVACAGPLLAAEIDAIGKALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAE
PDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQTATLLAHKLAAAGTIVWNGPV
GVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ

Sequences:

>Translated_396_residues
MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVMVTSHLGRPTEGEFKPEDSLA
PVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLNKGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIA
QFAPVACAGPLLAAEIDAIGKALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAE
PDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQTATLLAHKLAAAGTIVWNGPV
GVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ
>Mature_396_residues
MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVMVTSHLGRPTEGEFKPEDSLA
PVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLNKGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIA
QFAPVACAGPLLAAEIDAIGKALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAE
PDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQTATLLAHKLAAAGTIVWNGPV
GVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=38.1773399014778, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4505763, Length=406, Percent_Identity=36.9458128078818, Blast_Score=240, Evalue=2e-63,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=60.1010101010101, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=41.9512195121951, Blast_Score=273, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=39.6088019559902, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17136394, Length=399, Percent_Identity=40.6015037593985, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24581203, Length=400, Percent_Identity=37.5, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40956; Mature: 40956

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVM
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VTSHLGRPTEGEFKPEDSLAPVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLN
EEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
KGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIAQFAPVACAGPLLAAEIDAIG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHH
KALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAE
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCCC
PDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQT
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHEEECCCCCEEEEECCCH
ATLLAHKLAAAGTIVWNGPVGVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKY
HHHHHHHHHHCCEEEECCCCCEEEEHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHH
GIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ
CCCCCCCEEECCCHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVM
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VTSHLGRPTEGEFKPEDSLAPVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLN
EEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
KGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIAQFAPVACAGPLLAAEIDAIG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCHHHCCCHHHHHHHHHH
KALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAE
HHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCCC
PDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQT
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHEEECCCCCEEEEECCCH
ATLLAHKLAAAGTIVWNGPVGVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKY
HHHHHHHHHHCCEEEECCCCCEEEEHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHH
GIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ
CCCCCCCEEECCCHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA