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The map label for this gene is ansB [H]

Identifier: 94310348

GI number: 94310348

Start: 1518009

End: 1518977

Strand: Direct

Name: ansB [H]

Synonym: Rmet_1406

Alternate gene names: 94310348

Gene position: 1518009-1518977 (Clockwise)

Preceding gene: 94310347

Following gene: 94310349

Centisome position: 38.64

GC content: 69.45

Gene sequence:

>969_bases
ATGCCTCAAGCTGTTATCGCCATCCACGGTGGCGCGGGCACGATCACCCGTGCCGCGATGAATGCAGAGCGCGAACGCGA
GTACGCCGCCGCGCTCGAGCAGGTACTGCTCGCTGGCCAGCGCGTGCTGGCCGACGGCGGCAGCGCGCTCGACGCGGTCA
CCGAAGCGGTGCGCCTGCTCGAAGACAGTCCGCTGTTCAACGCCGGCCGGGGTGCCGTGCTGACCCATGCAGGCACTTTC
GAACTCGACGCGGCAATCATGGATGGCCAGACGCTGGGCGCCGGCGCCGTGGCCTGCGTCAAGCGCTTGCGCAACCCCGT
GCTGGCCGCGCGTGCCGTGCTGGAACGCAGCGAGCATGTGCTGTTTGCCGCCGAGGGCGCCGAGGCGTTTGCCGAAGCCC
AGGGGCTGGAACTGGTCGGGCCCGAGTACTACTTCACGCAGGCCCGCCACGATCAATGGGTGCGTGCGCAAGCCAGTACC
GGCATGGCGCTGCTCGACCATGACGCGGCTGCACTGGCCGCCAGGGCGGGGGCTGCCGATCCGATCGATCCCGACAACAA
GTTCGGCACGGTTGGCGCGGTGGCCTGCGATGCCGCTGGCAACCTCGCCGCGGCCACGTCGACCGGCGGCGTGACCAACA
AGCAGGTGGGCCGCGTGGGCGATACCCCGATCGTTGGGGCCGGCTGCTACGCGGACGATGTCGCGGCAGTCTCGGCCACG
GGCACGGGCGAGATGTTCATCCGCACCGTGGCCGCGTTCGACGTCTCGGCGCAAATGCGCTACGCCGGGCTGTCGCTCGA
GGAATCGGCGCGCCGCGTGGTGATGGAAAAGCTTCCCGCGATTGGCGGCCGTGGCGGCCTGATCGCGGTGGACCGTGCCG
GCAACGTCACGCTACCGTTCAACACGGAAGGCATGTACCGAGGCGTGGCACGCGTTGGCGAGCCGGCCACTGTCTGGATC
TACGGCTGA

Upstream 100 bases:

>100_bases
GAATTCCTGCTGCCCTGGCTCCACGGCGCGCCCGACGCGCGTGGTGGCCGGATTGCCCCGAAATCTCCCGACTGATCCCC
GACCGAAGTAGAAGGACCCC

Downstream 100 bases:

>100_bases
TATGGCAAAGACCGTTTCGAAACAAGGCATCGTGCTGCCGCCCGAGCGCGTGGTGGCCGTCGACAACCTCACCGTGCGCT
TCGCCACCTCGGAGCGTACC

Product: isoaspartyl dipeptidase with L-asparaginase activity

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPQAVIAIHGGAGTITRAAMNAEREREYAAALEQVLLAGQRVLADGGSALDAVTEAVRLLEDSPLFNAGRGAVLTHAGTF
ELDAAIMDGQTLGAGAVACVKRLRNPVLAARAVLERSEHVLFAAEGAEAFAEAQGLELVGPEYYFTQARHDQWVRAQAST
GMALLDHDAAALAARAGAADPIDPDNKFGTVGAVACDAAGNLAAATSTGGVTNKQVGRVGDTPIVGAGCYADDVAAVSAT
GTGEMFIRTVAAFDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNVTLPFNTEGMYRGVARVGEPATVWI
YG

Sequences:

>Translated_322_residues
MPQAVIAIHGGAGTITRAAMNAEREREYAAALEQVLLAGQRVLADGGSALDAVTEAVRLLEDSPLFNAGRGAVLTHAGTF
ELDAAIMDGQTLGAGAVACVKRLRNPVLAARAVLERSEHVLFAAEGAEAFAEAQGLELVGPEYYFTQARHDQWVRAQAST
GMALLDHDAAALAARAGAADPIDPDNKFGTVGAVACDAAGNLAAATSTGGVTNKQVGRVGDTPIVGAGCYADDVAAVSAT
GTGEMFIRTVAAFDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNVTLPFNTEGMYRGVARVGEPATVWI
YG
>Mature_321_residues
PQAVIAIHGGAGTITRAAMNAEREREYAAALEQVLLAGQRVLADGGSALDAVTEAVRLLEDSPLFNAGRGAVLTHAGTFE
LDAAIMDGQTLGAGAVACVKRLRNPVLAARAVLERSEHVLFAAEGAEAFAEAQGLELVGPEYYFTQARHDQWVRAQASTG
MALLDHDAAALAARAGAADPIDPDNKFGTVGAVACDAAGNLAAATSTGGVTNKQVGRVGDTPIVGAGCYADDVAAVSATG
TGEMFIRTVAAFDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNVTLPFNTEGMYRGVARVGEPATVWIY
G

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=312, Percent_Identity=37.1794871794872, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI145275200, Length=312, Percent_Identity=37.1794871794872, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI98991769, Length=273, Percent_Identity=32.6007326007326, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI285002251, Length=236, Percent_Identity=33.4745762711864, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI285002253, Length=236, Percent_Identity=31.3559322033898, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787050, Length=322, Percent_Identity=59.3167701863354, Blast_Score=354, Evalue=6e-99,
Organism=Drosophila melanogaster, GI18921183, Length=322, Percent_Identity=35.0931677018634, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI21357893, Length=270, Percent_Identity=32.962962962963, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24652135, Length=246, Percent_Identity=32.9268292682927, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI221330102, Length=246, Percent_Identity=32.9268292682927, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33096; Mature: 32964

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQAVIAIHGGAGTITRAAMNAEREREYAAALEQVLLAGQRVLADGGSALDAVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
EDSPLFNAGRGAVLTHAGTFELDAAIMDGQTLGAGAVACVKRLRNPVLAARAVLERSEHV
CCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCEE
LFAAEGAEAFAEAQGLELVGPEYYFTQARHDQWVRAQASTGMALLDHDAAALAARAGAAD
EEEECCHHHHHHHCCCEEECCCCEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHCCCCC
PIDPDNKFGTVGAVACDAAGNLAAATSTGGVTNKQVGRVGDTPIVGAGCYADDVAAVSAT
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECC
GTGEMFIRTVAAFDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNVTLPF
CCCHHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEC
NTEGMYRGVARVGEPATVWIYG
CCCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
PQAVIAIHGGAGTITRAAMNAEREREYAAALEQVLLAGQRVLADGGSALDAVTEAVRLL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
EDSPLFNAGRGAVLTHAGTFELDAAIMDGQTLGAGAVACVKRLRNPVLAARAVLERSEHV
CCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCEE
LFAAEGAEAFAEAQGLELVGPEYYFTQARHDQWVRAQASTGMALLDHDAAALAARAGAAD
EEEECCHHHHHHHCCCEEECCCCEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHCCCCC
PIDPDNKFGTVGAVACDAAGNLAAATSTGGVTNKQVGRVGDTPIVGAGCYADDVAAVSAT
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECC
GTGEMFIRTVAAFDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNVTLPF
CCCHHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEC
NTEGMYRGVARVGEPATVWIYG
CCCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]