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The map label for this gene is radA [H]

Identifier: 94310343

GI number: 94310343

Start: 1510447

End: 1511814

Strand: Direct

Name: radA [H]

Synonym: Rmet_1401

Alternate gene names: 94310343

Gene position: 1510447-1511814 (Clockwise)

Preceding gene: 94310342

Following gene: 94310345

Centisome position: 38.45

GC content: 67.32

Gene sequence:

>1368_bases
ATGGCCAAGACCAAGACGGTATACACATGCACCGAGTGCGGCGGCACCACGCCGCGCTGGGCGGGCCAGTGCCCACAGTG
CAACGCCTGGAACACGCTGGTGGAAACGGTGGCGGATTCGGGTTCGTCCGCGGCACGCCGCTTCCAGCCGCTGGCGACGT
CCGCTGTGGTGCGCAAGCTGTCGGAAATCGAGGCGGCCGATGTGCCACGCTTCTCGACCGGCATCGACGAATTCGACCGC
GTGCTTGGCGGCGGCCTGGTGTCGGGTGGGGTGGTGCTGATCGGCGGGGACCCCGGCATCGGTAAATCGACCCTGCTGCT
GCAGACGCTGGCCAACCTGTCGGCCAGCCGGCGCGTGCTCTATGTGAGCGGCGAGGAATCCGCCGCCCAGATCGCGCTGC
GCGCCCATCGCCTGGGCGTGGAGGCCGCCACGCTGGGCCTGCTGCCCGAGATTCAGTTGGAGAAGATCCAGGCCACGCTC
GAGGCCGACAAGCCCGAGGTGGCCGTGATCGACTCGATCCAGACGGTTTACTCGGAGGCGCTGACGTCCGCGCCGGGCTC
CGTGGCACAGGTACGCGAATGCGCGGCGCAACTGACGCGGATCGCCAAGAGTACCGGCATCACGATCATCCTGGTCGGCC
ACGTAACCAAGGAAGGCAGCCTGGCCGGCCCGCGCGTGCTCGAACACATCGTGGACACGGTGCTGTACTTCGAGGGGGAC
ACCCATTCCTCGCACCGCTTGATCCGGGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAACTCGGTGTCTTCGCGATGAC
CGAGCGCGGCCTGCGCGGCATCAGCAATCCGTCTGCGCTGTTCCTCTCCCAGCATGAGGAGCAGGTGGCCGGTTCCTGCG
TGCTGGTGACCCAGGAGGGCACGCGGCCATTGCTGGTCGAAGTGCAGGCGCTGGTCGACACCGCCAACGTGCCGAATCCG
CGCCGGCTGGCCGTGGGCCTCGAACAGAACCGGCTGGCGATGCTGCTGGCCGTACTGCATCGTCACGCGGGCATCGCGTG
TTTCGACCAGGACGTCTTCCTGAACGCGGTGGGCGGGGTCAAGATCACCGAGCCGGCCGCCGACCTGGCCGTGCTGCTGT
CGATTCATTCGTCGATGCGCAACAAGCCGCTGCCTCGCGGTCTGGTGGTGTTCGGCGAAGTGGGCCTGGCGGGAGAAATC
CGCCCGAGCCCGCGCGGACAGGAGCGGCTCAAGGAAGCCGCCAAGCTTGGTTTCACGATGGCGGTGATCCCGAAGGCCAA
TGCGCCCAAGCAGCGGATCGACGGCCTGGAGGTCATCGCGGTGGACCGGCTGGAACAGGCCATCGACCGGGTCAGGCATC
TGGACTGA

Upstream 100 bases:

>100_bases
GGCCGCCGCCAGCGGCACGGTTGGCTATGAACTGATGTGCGCGCTGGCGCCGCGCGTGCCGACTTCCGTTGCCACGTTGA
CCACCGCCGACGCCGTATAA

Downstream 100 bases:

>100_bases
CGTGGAAAGAAAAAAAGCCCCGCCGAAGCGGGGCTTTTTCATTGGCGCCTTCTCTCGCAGCCTGTGCTGCCGGATCAGCG
GTAGGTACCCACCACCCGCA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAKTKTVYTCTECGGTTPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLATSAVVRKLSEIEAADVPRFSTGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVLYVSGEESAAQIALRAHRLGVEAATLGLLPEIQLEKIQATL
EADKPEVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEEQVAGSCVLVTQEGTRPLLVEVQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEI
RPSPRGQERLKEAAKLGFTMAVIPKANAPKQRIDGLEVIAVDRLEQAIDRVRHLD

Sequences:

>Translated_455_residues
MAKTKTVYTCTECGGTTPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLATSAVVRKLSEIEAADVPRFSTGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVLYVSGEESAAQIALRAHRLGVEAATLGLLPEIQLEKIQATL
EADKPEVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEEQVAGSCVLVTQEGTRPLLVEVQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEI
RPSPRGQERLKEAAKLGFTMAVIPKANAPKQRIDGLEVIAVDRLEQAIDRVRHLD
>Mature_454_residues
AKTKTVYTCTECGGTTPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLATSAVVRKLSEIEAADVPRFSTGIDEFDRV
LGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVLYVSGEESAAQIALRAHRLGVEAATLGLLPEIQLEKIQATLE
ADKPEVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGDT
HSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEEQVAGSCVLVTQEGTRPLLVEVQALVDTANVPNPR
RLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIR
PSPRGQERLKEAAKLGFTMAVIPKANAPKQRIDGLEVIAVDRLEQAIDRVRHLD

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=454, Percent_Identity=58.3700440528634, Blast_Score=534, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48493; Mature: 48362

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTKTVYTCTECGGTTPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLATSAVVRKL
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SEIEAADVPRFSTGIDEFDRVLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVL
HHHHHHCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCEEE
YVSGEESAAQIALRAHRLGVEAATLGLLPEIQLEKIQATLEADKPEVAVIDSIQTVYSEA
EEECCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
LTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
HHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCC
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEEQVAGSCVLVTQEG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TRPLLVEVQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGV
CCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEECCCC
KITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIRPSPRGQERLKEAAKLGFTM
EEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEE
AVIPKANAPKQRIDGLEVIAVDRLEQAIDRVRHLD
EEEECCCCCHHHHCCEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTKTVYTCTECGGTTPRWAGQCPQCNAWNTLVETVADSGSSAARRFQPLATSAVVRKL
CCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SEIEAADVPRFSTGIDEFDRVLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLSASRRVL
HHHHHHCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCEEE
YVSGEESAAQIALRAHRLGVEAATLGLLPEIQLEKIQATLEADKPEVAVIDSIQTVYSEA
EEECCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
LTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
HHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCC
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEEQVAGSCVLVTQEG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TRPLLVEVQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGV
CCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEECCCC
KITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIRPSPRGQERLKEAAKLGFTM
EEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEE
AVIPKANAPKQRIDGLEVIAVDRLEQAIDRVRHLD
EEEECCCCCHHHHCCEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]