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The map label for this gene is cysB [H]

Identifier: 94310321

GI number: 94310321

Start: 1488185

End: 1489135

Strand: Direct

Name: cysB [H]

Synonym: Rmet_1379

Alternate gene names: 94310321

Gene position: 1488185-1489135 (Clockwise)

Preceding gene: 94310320

Following gene: 94310322

Centisome position: 37.89

GC content: 66.67

Gene sequence:

>951_bases
ATGAATTTCCAGCAACTGCGATCGATTCGCGAGGCGGTGCGCCGCCAGTTCAACCTCACCGAAGTGGCCAACGCGCTTTA
CACGTCGCAGCCCGGTGTGTCCCGCCAGATTCGCGAGCTCGAGGAAGAGCTGGGTGTGGAGATCTTCGAACGCTATGGCA
AGCGGCTGACCGGCCTGACCGAGCCCGGCCGGGAGATCGTCCGCATCGTCGAGCGCCTTCTGCTCGAAGCGGAGAACCTG
CGCCAGGCGGGCGACGAGTTCGCCGGCCGCCATACGGGCCGGCTGACTGTCGCCACCACCCACACGCAGGCGCGCTACGC
GCTGCCCAAGGTGGTGCAGAGCTTCCGGCGCGAGTATCCGCATGTGACGCTGGCGCTGCAGGAAGCGTCGCCGGCGCATA
TCGTCGAACTGCTGCTGACGGGGCAGGCCGATATCGGTATCGCCACCGAGGCCGTGGCCAGCGAAGCCGGTCTGACTTCG
TTCGAGGCCTATAGCTGGCAGCACGTGATCGTGGTGTCGCCCGACCATCCGCTGACGCGGCTGCCCGAGCCGACGCTGGA
GGACATCGCCAACTTCCCGCTCATTACCTACGACGCGGGCTTCACAGGCCGCCGCAAGATCGACGGTGCGTTCGCCGAGG
CCGGCCTGCAGCCGGAGATCGTGCTGACCGCGATGGACGCTGACGTCATCAAGACCTATGCCGAGCTGGAACTGGGCGTG
GGCATCATCGCGTCGATGGCGTATGACGCGCGCAAGGACGAAGGGCTGGTGCGGATCTCGGCCGACCATCTGTTCGAACC
GAACGTCACCAGCGTGGCCGTGCGACGCGGCGCTTATCTGCGCGGCTACGCCCATGCGTTCATCAACATGTTCGCGCCGC
ACCTGTCGCGCGACACGGTCTCCACGGCCCTGGCCGAATCCGCGCATCGCCCGGCGCTGGCTGCTGCCTGA

Upstream 100 bases:

>100_bases
GCCATCATGGTTTCAAGGAGGGCGAACTGCTGGCGGTACGGCCGCGGCAGTTGCGTGTCTTCAATCAGGGCCGGACAACA
CAAGAACAGGGAGCGGCACG

Downstream 100 bases:

>100_bases
GTTGGCGGCCCCGGCAGGCGTCGGGGCCATCGCCACGTTTGGCGCTTTCCGCTCGCTTGTCGGGCTCCACTACCTTTCGC
GCCATCTGTCGCCGGTTCGT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGDEFAGRHTGRLTVATTHTQARYALPKVVQSFRREYPHVTLALQEASPAHIVELLLTGQADIGIATEAVASEAGLTS
FEAYSWQHVIVVSPDHPLTRLPEPTLEDIANFPLITYDAGFTGRRKIDGAFAEAGLQPEIVLTAMDADVIKTYAELELGV
GIIASMAYDARKDEGLVRISADHLFEPNVTSVAVRRGAYLRGYAHAFINMFAPHLSRDTVSTALAESAHRPALAAA

Sequences:

>Translated_316_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGDEFAGRHTGRLTVATTHTQARYALPKVVQSFRREYPHVTLALQEASPAHIVELLLTGQADIGIATEAVASEAGLTS
FEAYSWQHVIVVSPDHPLTRLPEPTLEDIANFPLITYDAGFTGRRKIDGAFAEAGLQPEIVLTAMDADVIKTYAELELGV
GIIASMAYDARKDEGLVRISADHLFEPNVTSVAVRRGAYLRGYAHAFINMFAPHLSRDTVSTALAESAHRPALAAA
>Mature_316_residues
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLTEPGREIVRIVERLLLEAENL
RQAGDEFAGRHTGRLTVATTHTQARYALPKVVQSFRREYPHVTLALQEASPAHIVELLLTGQADIGIATEAVASEAGLTS
FEAYSWQHVIVVSPDHPLTRLPEPTLEDIANFPLITYDAGFTGRRKIDGAFAEAGLQPEIVLTAMDADVIKTYAELELGV
GIIASMAYDARKDEGLVRISADHLFEPNVTSVAVRRGAYLRGYAHAFINMFAPHLSRDTVSTALAESAHRPALAAA

Specific function: May be an accessory regulatory protein within the cys regulon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788296, Length=308, Percent_Identity=49.6753246753247, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=48.5245901639344, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=32.1554770318021, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789204, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=28.8065843621399, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=24.124513618677, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=23.8461538461538, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=28.0632411067194, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=203, Percent_Identity=25.615763546798, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082132, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788481, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=207, Percent_Identity=27.536231884058, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787601, Length=186, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34886; Mature: 34886

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCC
EPGREIVRIVERLLLEAENLRQAGDEFAGRHTGRLTVATTHTQARYALPKVVQSFRREYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
HVTLALQEASPAHIVELLLTGQADIGIATEAVASEAGLTSFEAYSWQHVIVVSPDHPLTR
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
LPEPTLEDIANFPLITYDAGFTGRRKIDGAFAEAGLQPEIVLTAMDADVIKTYAELELGV
CCCCHHHHHHCCCEEEECCCCCCCHHHCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GIIASMAYDARKDEGLVRISADHLFEPNVTSVAVRRGAYLRGYAHAFINMFAPHLSRDTV
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHH
STALAESAHRPALAAA
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNFQQLRSIREAVRRQFNLTEVANALYTSQPGVSRQIRELEEELGVEIFERYGKRLTGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCC
EPGREIVRIVERLLLEAENLRQAGDEFAGRHTGRLTVATTHTQARYALPKVVQSFRREYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
HVTLALQEASPAHIVELLLTGQADIGIATEAVASEAGLTSFEAYSWQHVIVVSPDHPLTR
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
LPEPTLEDIANFPLITYDAGFTGRRKIDGAFAEAGLQPEIVLTAMDADVIKTYAELELGV
CCCCHHHHHHCCCEEEECCCCCCCHHHCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GIIASMAYDARKDEGLVRISADHLFEPNVTSVAVRRGAYLRGYAHAFINMFAPHLSRDTV
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHH
STALAESAHRPALAAA
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8529872; 9097040; 9278503 [H]