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The map label for this gene is bktB [H]

Identifier: 94310304

GI number: 94310304

Start: 1471529

End: 1472713

Strand: Direct

Name: bktB [H]

Synonym: Rmet_1362

Alternate gene names: 94310304

Gene position: 1471529-1472713 (Clockwise)

Preceding gene: 94310303

Following gene: 94310305

Centisome position: 37.46

GC content: 64.89

Gene sequence:

>1185_bases
ATGGCACGTGAAGTGGTGGTTGTCAGCGGTGTGCGCACCGCAATCGGTACCTTCGGCGGCAGCCTGAAGGATCAGGCCCC
GACGGATATGGGCGCTCTGGTCGTGCGCGAAGCACTGGCCCGCGCCGGCGTTTCGGGCGATGACGTGGGTCATGTGGTGT
TCGGCAACGTGATCCAGACCGAGCCGAAGGACATGTATCTGGGCCGCGTGGCGGCTGTGAATGGTGGTGTGTCGATCAAC
GCGCCGGCACTGACCGTCAATCGCCTGTGCGGCTCCGGCCTGCAGGCAATCGTCAGCGCAGCCCAGACGATTCTGCTGGG
CGATGCCGACGTGGCAATCGGCGGCGGCGCGGAAAGCATGAGCCGCGCGCCGTACCTGGCGCCGATGGCCCGCTGGGGTG
CGCGCATGGGTGATGCCAAGCTGGTCGACATGATGCTTGGTGCACTGCACGATCCGTTCCACGCGATCCACATGGGCGTG
ACGGCGGAAAACGTCGCCAAGGAATATGACATTTCGCGCCAGCAGCAGGACGAAGCCGCGCTCGAATCGCATCGTCGTGC
TTCCGCCGCCATCAAGGCAGGCTATTTCAAGGATCAGATCGTTCCGGTTACGCTGAAATCGCGCAAGGGCGACGTGGTGT
TCGATACCGACGAGCACGTGCGTCACGATGCGACGATGGACGACATGACCAAGCTCAAGCCGGTGTTCGTCAAGGAGAAT
GGCACGGTCACGGCCGGTAACGCCTCGGGCCTGAACGACGCCGCCGCCGCCGTGGTGCTGATGGAGCGTGCCGAGGCCGA
GCGCCGCGGCCTGAAGCCGCTGGCTCGGCTGGTGTCCTACGGCCACGCCGGTGTCGATCCCAAGACGATGGGTATCGGTC
CGGTGCCGGCTACGAAGATCGCGCTGGAACGTGCCGGCCTGACGGTCAAGGATCTCGATGTGATCGAGGCCAACGAGGCA
TTCGCGGCGCAGGCCTGCGCGGTGACCAAGGCTTTGGGTCTCGACCCGGCGAAGGTCAACCCCAATGGCTCCGGTATCTC
GCTTGGCCACCCGATCGGCGCCACCGGTGCGCTCATCACGGTCAAGGCGCTGTACGAGCTGCAGCGTGTACAGGGTCGCT
ACGCGCTGGTGACGATGTGCATCGGTGGTGGCCAGGGCATTGCCGCGATCTTCGAGCGCGTTTAA

Upstream 100 bases:

>100_bases
AGCCGTACTTGTGCATACAATTAGGTAAAAGTACGGTCGTTCGTTTTCCCGGGGTCGGGTCTATACTGTGCGGCGCAACA
TGAACGCATGGAGACAAGAC

Downstream 100 bases:

>100_bases
AGTCGCGGGCAGTCAATCTGCCCGAGGAAGTCCGTCTTGTCCCGTCCCGTTCTGCTTTGCCTGTTGGCCGTCGCGATGCT
GTCTCTCCCAATTGCAGCGC

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQTEPKDMYLGRVAAVNGGVSIN
APALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGV
TAENVAKEYDISRQQQDEAALESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKEN
GTVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKIALERAGLTVKDLDVIEANEA
FAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVQGRYALVTMCIGGGQGIAAIFERV

Sequences:

>Translated_394_residues
MAREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQTEPKDMYLGRVAAVNGGVSIN
APALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGV
TAENVAKEYDISRQQQDEAALESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKEN
GTVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKIALERAGLTVKDLDVIEANEA
FAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVQGRYALVTMCIGGGQGIAAIFERV
>Mature_393_residues
AREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQTEPKDMYLGRVAAVNGGVSINA
PALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGVT
AENVAKEYDISRQQQDEAALESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKENG
TVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKIALERAGLTVKDLDVIEANEAF
AAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVQGRYALVTMCIGGGQGIAAIFERV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=47.0588235294118, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=45.8015267175573, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=41.2060301507538, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=41.9597989949749, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=36.4705882352941, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.2977099236641, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=49.748743718593, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=47.1938775510204, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=45.5223880597015, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=40.4522613065327, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=34.2298288508557, Blast_Score=211, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=46.2915601023018, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=35.8778625954198, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=32.311320754717, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=27.9012345679012, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=38.5749385749386, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=35.9102244389027, Blast_Score=218, Evalue=9e-58,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.9183673469388, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=45.1530612244898, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=40.8060453400504, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=33.7236533957845, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40996; Mature: 40864

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQT
CCCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHCC
EPKDMYLGRVAAVNGGVSINAPALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESM
CCCHHHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHEEEECCCCEEECCCHHHH
SRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGVTAENVAKEYDISRQQQDEAA
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHCCCHHHHHHHH
LESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKEN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCEEEEECC
GTVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKI
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALERAGLTVKDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEECCCEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRVQGRYALVTMCIGGGQGIAAIFERV
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
AREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQT
CCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHCC
EPKDMYLGRVAAVNGGVSINAPALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESM
CCCHHHHEEEEEECCCEEECCCHHHHHHHHCCHHHHHHHHHHEEEECCCCEEECCCHHHH
SRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGVTAENVAKEYDISRQQQDEAA
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHCCCHHHHHHHH
LESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKEN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCEEEEECC
GTVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKI
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
ALERAGLTVKDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEECCCEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRVQGRYALVTMCIGGGQGIAAIFERV
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]