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The map label for this gene is rimO [H]

Identifier: 94310303

GI number: 94310303

Start: 1470059

End: 1471429

Strand: Direct

Name: rimO [H]

Synonym: Rmet_1361

Alternate gene names: 94310303

Gene position: 1470059-1471429 (Clockwise)

Preceding gene: 94310302

Following gene: 94310304

Centisome position: 37.42

GC content: 62.73

Gene sequence:

>1371_bases
ATGTCGTCTGATACCAACTTGTCCAATCCGCCCAAAGTCGGCTTTGTTTCACTCGGCTGCCCCAAGGCCCTAGTGGACTC
CGAGCAGATCATTACCCAGCTCCGTGCCGAGGGATACTCGATCAGCGGCACCTATGACGGCGCCGACCTTGTGGTCGTCA
ATACGTGCGGCTTCATTGACGAGGCTGTGCAGGAGAGCCTGGACGCGATCGGCGAGGCGCTGACGGAGAACGGCAAGGTG
ATCGTGACCGGCTGCCTGGGCGCCAAGAAGGATGCCGCCGGGCACGACATCGTGAGTGCCGTGCATCCGAAGGTGCTGGC
TGTGACCGGCCCGCACGCGCTGGGCGAGGTGATGCAGGCGGTGCATACGCACCTGCCGAAGCCGCATGACCCGTTCACTG
ACCTGGTGCCGGCGGCCGGTATCAAGCTGACGCCGAAGCACTATGCCTACCTGAAGATTTCGGAAGGCTGTAACCATCGC
TGCTCGTTCTGCATCATCCCGTCGATGCGCGGCGACCTCGTCTCGCGCCCCGTGGCTGAAGTCATGCTCGAGGCCGAAAA
CCTGTTCAAGGCGGGCGTCAAGGAACTGCTGGTCATCTCGCAGGACACCAGCGCTTATGGCGTGGACGTCAAATATCGCA
CCGGTTTCTGGAATGGGCGCCCGCTCAAGACGCGCATGACGGAACTGGTGGCCGCGCTCGGCGAGCTGGCGAGCCAGTAT
GGTGCATGGGTTCGCCTGCACTACGTCTACCCGTATCCGCACGTCGACGAGATCATCCCGCTGATGAACCAGGGCAACGT
GCTGCCTTACCTCGATGTGCCGCTGCAGCATGCGCATCCGGATGTGCTCAAGCGCATGAAGCGCCCGGCGAATGCCGAGA
AGACACTCGACCGCATCCGCGCGTGGCGCGAAGTCTGCCCGGACCTGACGATTCGTAGCACGTTCATCGCCGGTTTCCCC
GGTGAGACCGAGGAAGAATTCCAGACGCTGCTCGATTTCATCGCCGAAGCCGAGCTTGACCGGGTGGGCTGCTTTGCCTA
TTCCCCGGTGGAAGGCGCCACGGCCAACGACCTGCCCGGTGCGTTGCCGGACGAAGTGCGCGAAGAGCGACGTGCCCGCT
TTATGGAAGTAGCCGAGGAGGTCTCCGCCCGCCGTCTTCAGCGCAAGGTCGGCCAGACGCTGCGCGTGCTGGTGGACGAG
GTCAATCAGGACGGTGGTATCGGCCGGTCGTCGGCGGATGCGCCGGAAATCGATGGTCTCGTCTATATCGATCCGGCGGC
CAAGGCTTCACAGCGCTACAAGACGGGCGACTTCGTCAATGTGAAGATTACCGGCGCCGACGGTCACGATCTTTGGGGTG
AAGTGGCGTAA

Upstream 100 bases:

>100_bases
TGACGGCGCCCGGCAGGCACGCCGGATGCCCTTGGCCCAGAGCGGCGCGCCACCGGGGACGGTCGGATGCGCCCCGAATC
CCCCCTATTTGCGGTGCCCC

Downstream 100 bases:

>100_bases
GCCGTACTTGTGCATACAATTAGGTAAAAGTACGGTCGTTCGTTTTCCCGGGGTCGGGTCTATACTGTGCGGCGCAACAT
GAACGCATGGAGACAAGACA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKV
IVTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHR
CSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQY
GAWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFP
GETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDE
VNQDGGIGRSSADAPEIDGLVYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA

Sequences:

>Translated_456_residues
MSSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKV
IVTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHR
CSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQY
GAWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFP
GETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDE
VNQDGGIGRSSADAPEIDGLVYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA
>Mature_455_residues
SSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVI
VTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRC
SFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQYG
AWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFPG
ETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDEV
NQDGGIGRSSADAPEIDGLVYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=420, Percent_Identity=26.9047619047619, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI28872782, Length=489, Percent_Identity=25.5623721881391, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI28872784, Length=492, Percent_Identity=26.6260162601626, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=64.4444444444444, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786882, Length=457, Percent_Identity=26.2582056892779, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=487, Percent_Identity=23.6139630390144, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=304, Percent_Identity=28.9473684210526, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356207, Length=489, Percent_Identity=26.7893660531697, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=25.974025974026, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49741; Mature: 49610

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFID
CCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHH
EAVQESLDAIGEALTENGKVIVTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHH
VHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAE
HHHHCCCCCCCHHHHHCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHH
VMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQY
HHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHC
GAWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIR
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
AWREVCPDLTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPG
HHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCC
ALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDEVNQDGGIGRSSADAPEIDGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA
EEECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFID
CCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHH
EAVQESLDAIGEALTENGKVIVTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHH
VHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAE
HHHHCCCCCCCHHHHHCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHH
VMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQY
HHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHC
GAWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIR
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
AWREVCPDLTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPG
HHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCC
ALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDEVNQDGGIGRSSADAPEIDGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA
EEECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA