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The map label for this gene is xylJ [H]

Identifier: 94310263

GI number: 94310263

Start: 1434835

End: 1435623

Strand: Reverse

Name: xylJ [H]

Synonym: Rmet_1321

Alternate gene names: 94310263

Gene position: 1435623-1434835 (Counterclockwise)

Preceding gene: 94310264

Following gene: 94310262

Centisome position: 36.55

GC content: 61.34

Gene sequence:

>789_bases
GTGATCATGGACAAAACTCAAATCACCCAGCTTGGCGACGCCCTGTATCAGGCACTGACCACGCGAACCGTGCTCGATCC
GCTGACCGAGCGCCACCCCGATATGACGATCGAGGACGCCTATCACATTCAGCAGCGCATGATCGAGCGTCGTGTGCAGG
CTGGTGAAACCATCATCGGCAAGAAGATCGGCGTCACGTCGGCGGCCGTGATGAACATGCTTGGCGTGTATCAGCCGGAT
TTCGGCTACATGCTCGACGGCATGATCTACAACGAAGGGCAGTCGATCGAGATCGGCACGCTGATTCAGCCCAAGGCCGA
AGGCGAGATCGCGTTCATCCTCAAGAAAGACCTGATGGGGCCGGGGGTGACCAACGTGGACGTGCTGGCGGCGACCGAAT
GCGTGATGCCGTGCTTTGAAATTGTCGATTCGCGCATCCGCGACTGGAAGATCAAGATTCAGGACACCGTGGCGGACAAC
GCCTCCTGTGGCGTATTCGTGCTGGGTGATCACGCGGTCGATCCACGCAAGCTCGACCTGACGCTGTGCGGCATGGTGAT
GGAGCGTAATGGCGAGATTATCGCCACGGGCTGCGGCGCGGCGGCACTGGGCTCACCGGTCAACGCCGTGGCCTGGCTTG
CCAACACGCTGGGGCGTCTGGGCATTGCACTCAAGGCGGGCGAGGTGATCTTGTCCGGCGCGCTGGCGGCGATGTTCCCG
GCCAAGGCGGGCGACAGCTTCCGCGTCAGTATTGGTGGTCTGGGCGGTTGTTCGGTCCGGTTTCACTGA

Upstream 100 bases:

>100_bases
GCACGCCGTTCGGCGGGGCCAAGCAGTCGGGTATCGGGCGCGAGGGCGGAGTCCACTCGCTGGAGTTCTACACGGAACTC
AAAAACGTTTGCATCAAACT

Downstream 100 bases:

>100_bases
ACGCGCCTGGCCAGGCACCACGCCCGGCCGTTGTCGGATGCCGTGCGTAAAAGTCATTTTTATAGGAAGGACAAGGTCAT
GAAAAAGATCAAATGTGCGC

Product: 2-hydroxypent-2,4-dienoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIMDKTQITQLGDALYQALTTRTVLDPLTERHPDMTIEDAYHIQQRMIERRVQAGETIIGKKIGVTSAAVMNMLGVYQPD
FGYMLDGMIYNEGQSIEIGTLIQPKAEGEIAFILKKDLMGPGVTNVDVLAATECVMPCFEIVDSRIRDWKIKIQDTVADN
ASCGVFVLGDHAVDPRKLDLTLCGMVMERNGEIIATGCGAAALGSPVNAVAWLANTLGRLGIALKAGEVILSGALAAMFP
AKAGDSFRVSIGGLGGCSVRFH

Sequences:

>Translated_262_residues
MIMDKTQITQLGDALYQALTTRTVLDPLTERHPDMTIEDAYHIQQRMIERRVQAGETIIGKKIGVTSAAVMNMLGVYQPD
FGYMLDGMIYNEGQSIEIGTLIQPKAEGEIAFILKKDLMGPGVTNVDVLAATECVMPCFEIVDSRIRDWKIKIQDTVADN
ASCGVFVLGDHAVDPRKLDLTLCGMVMERNGEIIATGCGAAALGSPVNAVAWLANTLGRLGIALKAGEVILSGALAAMFP
AKAGDSFRVSIGGLGGCSVRFH
>Mature_262_residues
MIMDKTQITQLGDALYQALTTRTVLDPLTERHPDMTIEDAYHIQQRMIERRVQAGETIIGKKIGVTSAAVMNMLGVYQPD
FGYMLDGMIYNEGQSIEIGTLIQPKAEGEIAFILKKDLMGPGVTNVDVLAATECVMPCFEIVDSRIRDWKIKIQDTVADN
ASCGVFVLGDHAVDPRKLDLTLCGMVMERNGEIIATGCGAAALGSPVNAVAWLANTLGRLGIALKAGEVILSGALAAMFP
AKAGDSFRVSIGGLGGCSVRFH

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=259, Percent_Identity=40.9266409266409, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 28089; Mature: 28089

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMDKTQITQLGDALYQALTTRTVLDPLTERHPDMTIEDAYHIQQRMIERRVQAGETIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECC
KKIGVTSAAVMNMLGVYQPDFGYMLDGMIYNEGQSIEIGTLIQPKAEGEIAFILKKDLMG
CCCCHHHHHHHHHHHCCCCCCCCEECCEEECCCCCEEEEEEECCCCCCCEEEEEEHHCCC
PGVTNVDVLAATECVMPCFEIVDSRIRDWKIKIQDTVADNASCGVFVLGDHAVDPRKLDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEEECCCCCCCEEHH
TLCGMVMERNGEIIATGCGAAALGSPVNAVAWLANTLGRLGIALKAGEVILSGALAAMFP
HEEHHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHCC
AKAGDSFRVSIGGLGGCSVRFH
CCCCCEEEEEECCCCCCEEEEC
>Mature Secondary Structure
MIMDKTQITQLGDALYQALTTRTVLDPLTERHPDMTIEDAYHIQQRMIERRVQAGETIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECC
KKIGVTSAAVMNMLGVYQPDFGYMLDGMIYNEGQSIEIGTLIQPKAEGEIAFILKKDLMG
CCCCHHHHHHHHHHHCCCCCCCCEECCEEECCCCCEEEEEEECCCCCCCEEEEEEHHCCC
PGVTNVDVLAATECVMPCFEIVDSRIRDWKIKIQDTVADNASCGVFVLGDHAVDPRKLDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEEECCCCCCCEEHH
TLCGMVMERNGEIIATGCGAAALGSPVNAVAWLANTLGRLGIALKAGEVILSGALAAMFP
HEEHHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHCC
AKAGDSFRVSIGGLGGCSVRFH
CCCCCEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]