Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 94310244

GI number: 94310244

Start: 1415972

End: 1416667

Strand: Reverse

Name: alkJ [H]

Synonym: Rmet_1300

Alternate gene names: 94310244

Gene position: 1416667-1415972 (Counterclockwise)

Preceding gene: 94310247

Following gene: 94310242

Centisome position: 36.07

GC content: 68.68

Gene sequence:

>696_bases
GTGCAGGGCGCCAAGACCCTCAACACCCTGGCCAGCTCGCTCTGGGGCAAGGCCCTGATCGGACTGGAATATGCGCTCAG
GCGCAGCGGACCCATGAGCATGGCGCCCAGCCAGCTCGGGGCATTCACCAAGAGCGACCCGTCACAGCCGCATGCCAACA
TCGAATACCACGTGCAGCCGCTGAGTCTGGATGCCTTCGGTGAGCCGCTGCATCCGTTTCCGGCCTTCACCGCCAGCGTC
TGCAACCTCAACCCTGGCAGCCGCGGACATGTGCGCATCCGCTCGCCGCGCTGCGAGGACGCGCCGGCCATCGCGCCCAA
CTACCTGAGCACCGACGAAGACCGCAAGGTGGCGGCCGACAGCCTGCGCGTGACCCGCCACATCGTGGGCCAGCCCGCCA
TGGCCAAATACCGGCCCGAAGAGTTCAAGCCCGGGCCCCAGTACCAGAGCGACGAGGAGCTGGCGCGCCTGGCCGGCGAC
ATCGCCAGCACCATTTTCCACCCGGTGGGCACGACGAAGATGGGGCGCGACGGTGATCCGATGGCGGTGGTCGACAGCCA
CCTGCGGGTGCGCGGCGTGGCCGGCCTGCGGGTGGTGGATGCGGGCGTCATGCCCACCATCACCAGCGGCAACACCAACA
GCCCGACGCTGATGATCGCGGAGAAGGCGGCGCGCTGGATCCTGACCGGGGCCTGA

Upstream 100 bases:

>100_bases
TGACCTCTCGGGCCAAGAGTAAACCACGGCGTGAACCAATTAACCAATCATCAGGGACACGACACTGCAGGACCACCTGC
AGATCCGCTCGGTCTACAAG

Downstream 100 bases:

>100_bases
AGTGCTGCGGCCGGTTCAGCGCAGCCGACCGATGTAGCGGCTGGCCAGGGCCGCCTGTGCGCTTGAAAGCGGGCCTTCTT
CGGCCGGACCACTGGCGGGC

Product: choline dehydrogenase (CDH) (partial)(belongs to CMGI-2)

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQGAKTLNTLASSLWGKALIGLEYALRRSGPMSMAPSQLGAFTKSDPSQPHANIEYHVQPLSLDAFGEPLHPFPAFTASV
CNLNPGSRGHVRIRSPRCEDAPAIAPNYLSTDEDRKVAADSLRVTRHIVGQPAMAKYRPEEFKPGPQYQSDEELARLAGD
IASTIFHPVGTTKMGRDGDPMAVVDSHLRVRGVAGLRVVDAGVMPTITSGNTNSPTLMIAEKAARWILTGA

Sequences:

>Translated_231_residues
MQGAKTLNTLASSLWGKALIGLEYALRRSGPMSMAPSQLGAFTKSDPSQPHANIEYHVQPLSLDAFGEPLHPFPAFTASV
CNLNPGSRGHVRIRSPRCEDAPAIAPNYLSTDEDRKVAADSLRVTRHIVGQPAMAKYRPEEFKPGPQYQSDEELARLAGD
IASTIFHPVGTTKMGRDGDPMAVVDSHLRVRGVAGLRVVDAGVMPTITSGNTNSPTLMIAEKAARWILTGA
>Mature_231_residues
MQGAKTLNTLASSLWGKALIGLEYALRRSGPMSMAPSQLGAFTKSDPSQPHANIEYHVQPLSLDAFGEPLHPFPAFTASV
CNLNPGSRGHVRIRSPRCEDAPAIAPNYLSTDEDRKVAADSLRVTRHIVGQPAMAKYRPEEFKPGPQYQSDEELARLAGD
IASTIFHPVGTTKMGRDGDPMAVVDSHLRVRGVAGLRVVDAGVMPTITSGNTNSPTLMIAEKAARWILTGA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=208, Percent_Identity=40.8653846153846, Blast_Score=157, Evalue=6e-39,
Organism=Escherichia coli, GI1786503, Length=217, Percent_Identity=40.0921658986175, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17532301, Length=206, Percent_Identity=34.9514563106796, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45551458, Length=236, Percent_Identity=32.6271186440678, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45549471, Length=236, Percent_Identity=32.6271186440678, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24642042, Length=237, Percent_Identity=35.0210970464135, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24642055, Length=173, Percent_Identity=39.3063583815029, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24642059, Length=235, Percent_Identity=33.1914893617021, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859993, Length=158, Percent_Identity=36.7088607594937, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24642051, Length=235, Percent_Identity=28.5106382978723, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24642048, Length=170, Percent_Identity=34.7058823529412, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18859995, Length=151, Percent_Identity=36.4238410596026, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137792, Length=231, Percent_Identity=29.8701298701299, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24642035, Length=160, Percent_Identity=31.875, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645930, Length=169, Percent_Identity=35.5029585798817, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24642039, Length=203, Percent_Identity=35.9605911330049, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24650267, Length=151, Percent_Identity=32.4503311258278, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24642057, Length=168, Percent_Identity=36.3095238095238, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642037, Length=158, Percent_Identity=30.379746835443, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 24796; Mature: 24796

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGAKTLNTLASSLWGKALIGLEYALRRSGPMSMAPSQLGAFTKSDPSQPHANIEYHVQP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEE
LSLDAFGEPLHPFPAFTASVCNLNPGSRGHVRIRSPRCEDAPAIAPNYLSTDEDRKVAAD
EEHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
SLRVTRHIVGQPAMAKYRPEEFKPGPQYQSDEELARLAGDIASTIFHPVGTTKMGRDGDP
HHHHHHHHHCCCHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
MAVVDSHLRVRGVAGLRVVDAGVMPTITSGNTNSPTLMIAEKAARWILTGA
HHHHHCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEECCCCEEECCC
>Mature Secondary Structure
MQGAKTLNTLASSLWGKALIGLEYALRRSGPMSMAPSQLGAFTKSDPSQPHANIEYHVQP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEE
LSLDAFGEPLHPFPAFTASVCNLNPGSRGHVRIRSPRCEDAPAIAPNYLSTDEDRKVAAD
EEHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
SLRVTRHIVGQPAMAKYRPEEFKPGPQYQSDEELARLAGDIASTIFHPVGTTKMGRDGDP
HHHHHHHHHCCCHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
MAVVDSHLRVRGVAGLRVVDAGVMPTITSGNTNSPTLMIAEKAARWILTGA
HHHHHCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]