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The map label for this gene is tnpB [H]

Identifier: 94310207

GI number: 94310207

Start: 1384634

End: 1385440

Strand: Reverse

Name: tnpB [H]

Synonym: Rmet_1263

Alternate gene names: 94310207

Gene position: 1385440-1384634 (Counterclockwise)

Preceding gene: 94310208

Following gene: 94310206

Centisome position: 35.27

GC content: 58.61

Gene sequence:

>807_bases
ATGGACATCCAGCACGAACGTATCCTCGAAGCCTGTCGACAACTGCGGATCGACACGATGGGCGAGCAGTACAGCATGTT
GGCAGCTCGCGCGGCAGCGAACCAACTCACGTTTGCCGACTTCTTCGAGGCGCTGTTGAAATCTGCGATGGACGCGAAGC
ACACGCGGACCAAATCCATGTTGCTGCGCACGGCCGGCTTCCCTGTGCTGAAGACGCTGGAGGAGTTCGACTTTACGTTT
GCTCACGGCGTTGCCAAGAAATCGGTGCTGGAGCTGTCGTCGATGGCCTTCGTCGAGCGCGCCGAGAACATCGTGTTGCT
CGGGCCGAGCGGCCTTGGCAAAACGCATCTGGCGATCGCACTCGGTTACATCGCAACGCAGATGGGGATCAAGACGCGCT
TCATCTCTGCGGCGGATCTCGTCATTGCCTTGGCCGCCGCTTCCCGGCAGGATCGATTGGCCGACTTCCTGAAGCGCAAC
ATCATGAACCCACGGCTGTTGATCATTGATGAGGTCGGCTATCTGCCGCTCGGCCGGGACGATGCCAATCTGTTCTTCCA
GGTGATCGCCAAGCGGTATGAAAAGGGTTCCGTGATCGTAACGAGCAACTTGCCGTTCGGGCAATGGGACCAGACGTTCG
CCGGAGATCAGACGCTCACGGCCGCGTTGCTCGACCGCCTCCTGCATCACAGCCATGTTCTACAGATCAAAGGCGAAAGC
TACCGACTGAAGGACAAGCGAAAAGCCGGCGTGATCCGATCCCGTGCATCCGAAAGTGCTGAACAGCAACTGGAGATGCA
CGCGTAA

Upstream 100 bases:

>100_bases
CAGCTTCCGTGCCGACGATGCCACCGCCGTCGCGGTTCGATGATACGCCGCTGCAGCATCAGCTGTCAGTGTATCAAGCC
CTGCTGACGGAGGCACGTTG

Downstream 100 bases:

>100_bases
GTGATGATCAGCAACATCGTTTGACCCTGGGTCAGGCGAAACCGATGGCCAGCAACTGACGCTGACCCAGGGTCACGCAA
CATGCTGGGCATCAACGGGA

Product: transposase/IS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSMLLRTAGFPVLKTLEEFDFTF
AHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRN
IMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGES
YRLKDKRKAGVIRSRASESAEQQLEMHA

Sequences:

>Translated_268_residues
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSMLLRTAGFPVLKTLEEFDFTF
AHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRN
IMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGES
YRLKDKRKAGVIRSRASESAEQQLEMHA
>Mature_268_residues
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSMLLRTAGFPVLKTLEEFDFTF
AHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRN
IMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGES
YRLKDKRKAGVIRSRASESAEQQLEMHA

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29893; Mature: 29893

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLRTAGFPVLKTLEEFDFTFAHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
LGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRNIMNPRLLIIDEVGYLPLGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
DANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGES
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCC
YRLKDKRKAGVIRSRASESAEQQLEMHA
EECCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLRTAGFPVLKTLEEFDFTFAHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
LGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRNIMNPRLLIIDEVGYLPLGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
DANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGES
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCC
YRLKDKRKAGVIRSRASESAEQQLEMHA
EECCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]