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The map label for this gene is eno

Identifier: 94310121

GI number: 94310121

Start: 1287368

End: 1288645

Strand: Direct

Name: eno

Synonym: Rmet_1176

Alternate gene names: 94310121

Gene position: 1287368-1288645 (Clockwise)

Preceding gene: 94310119

Following gene: 94310123

Centisome position: 32.77

GC content: 64.63

Gene sequence:

>1278_bases
ATGAGCAAGGTGGCTGAGATCCGGGGGCTGGAGGTGCTCGATAGTCGGGGGAATCCGACGGTGGAGGTGGAGGTCGTGCT
CGACGACGGTGCGGTAGGCCGTGCCATTGTGCCGTCGGGGGCATCGACCGGCGCGCGCGAGGCCGTGGAGTTGCGCGATG
CCGATCCGCGACGCTATTTGGGGCGAGGCGTGTTGCGGGCGGTTCAGGCCGTCAACACCGAGTTGTGTGAGGCACTGCTC
GGGCGTGATGCCGGTGACCAACCCGAGATCGACACGATCATGATCGACCTCGACGGCACCCCGGATAAGCGTCGGCTTGG
CGCCAATGCGTTGCTCGGCGTGTCCCTGGCCGTGGCTCACGCGGCTGCGATGTCGAATGGTCTGCCGCTGTTCGCCTATC
TTGGCGGAGAGCGCGCTTCGTTGCTGCCCGTGCCGCTGATCAACGTGATCAATGGTGGAGCCCATGCCGACAATGCGCTC
GATTTTCAGGAATTCATGATCGTGCCTGCCGGGGCACCGACCTTTACCGAGGCGGTACGTGCCAGCGCGGAGGTTTTCCA
TACGCTACGCGCCATGCTGAAGGCTGCCGGCTACACCACCAACGTGGGCGACGAGGGTGGCTTCGCGCCCGAATTTCACG
CTGCCGAGGAGGCGTTGGACATTCTGGTCGCAGCCATCGCCAAGGCAGGCTATCGCCCCGGCGAGGACATCGCGCTGGCC
ATCGACCCTGCCGCCAGCGAGCTATATGTCAATGGCAGCTATGTCTATCAGGGCGAGGGCGTCGAGCGCTCGCGCGAGGA
GCAGGTGGCCTATCTGGTACGCCTCGCGGACCGCTATCCGATTGTTTCGATCGAGGATGGCATGGCGGAAGACGATGCGA
TGGGCTGGCAATTGCTGACGCGGCAGCTCGGCAAGCGGTGCCAGCTTGTTGGCGACGATGTGTTCTGCACACATCCGACC
TTGCTGAGGCAGGGGGTAGAGCAGGGCATGGCCAACGCCATCCTGGTCAAGGCCAACCAGATCGGCACGCTGAGCGAGAT
GCGCGAGACGGTTCGCGTGGCCCATGGTTACGCGTATTCGGCCGTGATGTCGCATCGTTCGGGTGAAACCGAGGATGTGA
CGATCGCCGACCTTGCTGTGGCGCTGCGATGCGGGCAGATCAAGACGGGGTCGATGTCGCGCGCGGATCGCACTGCCAAG
TACAACCGCCTGCTGCGCATCGAGCGCGAGCTTGGGTCGCGTGCCGAGTACGCGGGCGCATTGCTGCGGCGCCGCTAG

Upstream 100 bases:

>100_bases
AGAAAAGCTGACGAATTGAGGAAAATAACGCGATATTCAGCTTGGTCAAAGCCTTTTAGGATCGACGAATCTTCATTTTT
CTCACAAAGGGAGTGCGCGA

Downstream 100 bases:

>100_bases
AGCACTAGAGCAGCGCGAAGCGCGCGGCCTGTGGCCCGATCACGGCGGGGCGGCTGACCGGGTAGCGATGGTGGTCGCCG
CCATCGCTGAACGAGAGGTA

Product: enolase (2-phosphoglycerate dehydratase 2) (2-phospho- D-glycerate hydro-lyase 2)

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYLGRGVLRAVQAVNTELCEALL
GRDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAHAAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNAL
DFQEFMIVPAGAPTFTEAVRASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALA
IDPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLTRQLGKRCQLVGDDVFCTHPT
LLRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYSAVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAK
YNRLLRIERELGSRAEYAGALLRRR

Sequences:

>Translated_425_residues
MSKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYLGRGVLRAVQAVNTELCEALL
GRDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAHAAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNAL
DFQEFMIVPAGAPTFTEAVRASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALA
IDPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLTRQLGKRCQLVGDDVFCTHPT
LLRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYSAVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAK
YNRLLRIERELGSRAEYAGALLRRR
>Mature_424_residues
SKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYLGRGVLRAVQAVNTELCEALLG
RDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAHAAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNALD
FQEFMIVPAGAPTFTEAVRASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALAI
DPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLTRQLGKRCQLVGDDVFCTHPTL
LRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYSAVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAKY
NRLLRIERELGSRAEYAGALLRRR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=46.189376443418, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=47.3441108545035, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=47.5638051044084, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=47.5638051044084, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=43.3566433566434, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI310129182, Length=121, Percent_Identity=33.0578512396694, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI310110045, Length=121, Percent_Identity=33.0578512396694, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI310120572, Length=121, Percent_Identity=33.0578512396694, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=55.6603773584906, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=46.8965517241379, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=46.8965517241379, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=40.7216494845361, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=45.852534562212, Blast_Score=356, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=47.0997679814385, Blast_Score=355, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=45.6221198156682, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=45.6221198156682, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=46.5277777777778, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=46.4367816091954, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO2_RALME (Q1LP64)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583331.1
- ProteinModelPortal:   Q1LP64
- SMR:   Q1LP64
- STRING:   Q1LP64
- GeneID:   4037976
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1176
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   TANGGIQ
- BioCyc:   RMET266264:RMET_1176-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45532; Mature: 45401

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYL
CCCCHHHCCCCEECCCCCCEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHH
GRGVLRAVQAVNTELCEALLGRDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNALDFQEFMIVPAGAPTFTEAVR
HHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
ASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IDPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLT
ECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
RQLGKRCQLVGDDVFCTHPTLLRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYS
HHHCCHHEECCCCEEECCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHH
AVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAKYNRLLRIERELGSRAEYAGA
HHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLRRR
HHHCC
>Mature Secondary Structure 
SKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYL
CCCHHHCCCCEECCCCCCEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHH
GRGVLRAVQAVNTELCEALLGRDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNALDFQEFMIVPAGAPTFTEAVR
HHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
ASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IDPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLT
ECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
RQLGKRCQLVGDDVFCTHPTLLRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYS
HHHCCHHEECCCCEEECCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHH
AVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAKYNRLLRIERELGSRAEYAGA
HHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLRRR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA