Click here to switch to the map view.

The map label for this gene is yhcK [H]

Identifier: 94310036

GI number: 94310036

Start: 1185361

End: 1187322

Strand: Reverse

Name: yhcK [H]

Synonym: Rmet_1091

Alternate gene names: 94310036

Gene position: 1187322-1185361 (Counterclockwise)

Preceding gene: 94310042

Following gene: 94310035

Centisome position: 30.23

GC content: 59.38

Gene sequence:

>1962_bases
ATGCTGACTTCGCGCGACAAATCGCCTTTGGCATGGGGGAAGATCCGCCACGTCCAGGTGAGTGAGCAAGTCATTTCTGA
CCAATCAGTAGACGTTTCATCAGCGGGAATCATGGGCCATTCACAGAGTTCAGCTACTTACTGGTCGATGGATCGGCGCA
TGAAGAAGTCGTGGCTTCGGCGGGCGTTACCTATTCTGGCCCTGCTGGTGGCCATAGATATCCTGGTGAATGTATGGCAG
TCCTACTATGACTTTCGTGCAATCAACGCCCTGGCCGCCCGGCTACTGGATGTGCAATCTTCCCTGATGGCCGGACGTAT
TGCAGATCAGTTTCGCGACGTAACTGCGTTAACGAGCGGAACGGAGCGTCTGCTCGATACGGGCACACTCACGGACGCCA
CACTTGTCAATCTCACCGAGGCTCTGGATAACCCATTGAGACGCGCGCTAATCGGGGTGTTCGATCCGCAGGGTGAGCAT
CTGCTTGCCAGTTCCCGTCCAAGCTTCATGAACGAAGTGCCGCCGCTTGACCAGATTTTGGCAAGAGTGCGAGCCAATCC
GTCAAACGCCGTCATACTCCCCGTCGCGTGGGGCGAGCGTGGCGCGGTCGTGGTCGCCAAAGGGCATCAGAACCGGGCGA
TCGACCTTGATGCGGTGGTCGTCATCATCCTGACCTACGAACGCCTTGTGCTTGACGGCGTGAACCTGGCACCCGGCACC
TCCGTTCTGCTATTGGATAGCGAACACCGCCTGGTGATGCGTGATCCGGAGCATCCGACACTAGCCGTTGGACAGGTCCT
GCGTGAAGATCGCGGGCGGGCCGGACCAACAACCGCAACGCAATATGCTTCCTCGCAAATCGACGGTATCGAGCGTTTGG
TGTCCACACGAAGCATTGCGACACGGCTGACCCCCGACCACTGGACGGTGGCGGTCGGATATTCGGTCAGCGATTTCCGG
GAGGGCTTCTGGCGGAGCACCTACATCAATGCGGCCGGCGCCGCTGTCATGCTTCTCATGTTGGTGAGTGGCATTCTGCT
GATCCTCCACGAGCGTCGTCTGCAGGATCGGGTCGAGAGATTCGCGAGCATGGTGTCAACCGTCGTCAAGAACATGCCGA
CGCCCGTTGCCGTAGTCGACCATGACACGGAACAGATCCTCCTGACTAACGAAGCCCTACTCGCTGACTTCGGCGCTGTG
GCCGGAGCGGGGCAATCATTCTCCCGGCTCTTTGCGAACGCCGCAGACTGGTCGCAGGTATGTACGACCCGTATGGATGA
AGCCATTCCCATGCTCACGCGCGACGGAACGCGATACATGCTGGTGCATTGTGCTCAGCTTGAGTTTGGAGGTGAATCAG
TCGCGTCAAAAGCGGTGCTTGTCACACTGGTCGACGTCAACAGCCAGCAACAGATGCTCAAGGAGTTGCGAACCCAAGCC
GACCTCGATGCACTGACCGGGCTCGCCAACCGCCGCTATTTCGAAAACGCAGCCCAGAAGGCAGTCATGCACGCGCGCAA
GCAAGGCAGTGCCTTGTCGATACTCGCGCTCGATCTTGATCTATTCAAGCGGGTGAATGATACCTACGGCCATGCCGCTG
GCGATCGCGTTTTACAGGTGGTGGCGCGGCAATTCGAGGGCGCATTGCGCAGCACTGACCTTGGCGCGCGAATTGGCGGA
GAAGAGTTCACCGCAATCCTGTTCGATACAGCGCTGGGACAGGCGCAGGTGATCGCCGAGCGCATCCGCGCTGCCATCCA
GAACACGCCGATCATTCTTGAGTCCGGAGAAGCAATCACGCAGACCGTGAGTATTGGCATCGCGGCGTACCGCGAGGACG
AATCCGACCTCGCGGCAGCGCAGGAACGGGCGGACGCCGCCCTCTACAGTGCCAAGGCAGCGGGGCGAAACCGCGTTCAT
GTGTATGTGCCCGAGACGTCTGCGCCCCAGGATGCCGGCTAG

Upstream 100 bases:

>100_bases
CCGCGTGGACATATGATTGATTGGCGCGCCGGCTGGTGAGAGTCAATATGCACGTGGGATCGGGCATCCACGCGTGCCGC
AAGCAGCACCTTGCCTACAT

Downstream 100 bases:

>100_bases
GTACGGAATATCGGTTTGACCATTGCAAACGGCCGCCTGGCCTGGGTGGCTCTGGATACGCATGGGGTGCGGACGACAAA
TGTAGGCAGAGAGCCTCACC

Product: putative diguanylate cyclase (GGDEF domain)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MLTSRDKSPLAWGKIRHVQVSEQVISDQSVDVSSAGIMGHSQSSATYWSMDRRMKKSWLRRALPILALLVAIDILVNVWQ
SYYDFRAINALAARLLDVQSSLMAGRIADQFRDVTALTSGTERLLDTGTLTDATLVNLTEALDNPLRRALIGVFDPQGEH
LLASSRPSFMNEVPPLDQILARVRANPSNAVILPVAWGERGAVVVAKGHQNRAIDLDAVVVIILTYERLVLDGVNLAPGT
SVLLLDSEHRLVMRDPEHPTLAVGQVLREDRGRAGPTTATQYASSQIDGIERLVSTRSIATRLTPDHWTVAVGYSVSDFR
EGFWRSTYINAAGAAVMLLMLVSGILLILHERRLQDRVERFASMVSTVVKNMPTPVAVVDHDTEQILLTNEALLADFGAV
AGAGQSFSRLFANAADWSQVCTTRMDEAIPMLTRDGTRYMLVHCAQLEFGGESVASKAVLVTLVDVNSQQQMLKELRTQA
DLDALTGLANRRYFENAAQKAVMHARKQGSALSILALDLDLFKRVNDTYGHAAGDRVLQVVARQFEGALRSTDLGARIGG
EEFTAILFDTALGQAQVIAERIRAAIQNTPIILESGEAITQTVSIGIAAYREDESDLAAAQERADAALYSAKAAGRNRVH
VYVPETSAPQDAG

Sequences:

>Translated_653_residues
MLTSRDKSPLAWGKIRHVQVSEQVISDQSVDVSSAGIMGHSQSSATYWSMDRRMKKSWLRRALPILALLVAIDILVNVWQ
SYYDFRAINALAARLLDVQSSLMAGRIADQFRDVTALTSGTERLLDTGTLTDATLVNLTEALDNPLRRALIGVFDPQGEH
LLASSRPSFMNEVPPLDQILARVRANPSNAVILPVAWGERGAVVVAKGHQNRAIDLDAVVVIILTYERLVLDGVNLAPGT
SVLLLDSEHRLVMRDPEHPTLAVGQVLREDRGRAGPTTATQYASSQIDGIERLVSTRSIATRLTPDHWTVAVGYSVSDFR
EGFWRSTYINAAGAAVMLLMLVSGILLILHERRLQDRVERFASMVSTVVKNMPTPVAVVDHDTEQILLTNEALLADFGAV
AGAGQSFSRLFANAADWSQVCTTRMDEAIPMLTRDGTRYMLVHCAQLEFGGESVASKAVLVTLVDVNSQQQMLKELRTQA
DLDALTGLANRRYFENAAQKAVMHARKQGSALSILALDLDLFKRVNDTYGHAAGDRVLQVVARQFEGALRSTDLGARIGG
EEFTAILFDTALGQAQVIAERIRAAIQNTPIILESGEAITQTVSIGIAAYREDESDLAAAQERADAALYSAKAAGRNRVH
VYVPETSAPQDAG
>Mature_653_residues
MLTSRDKSPLAWGKIRHVQVSEQVISDQSVDVSSAGIMGHSQSSATYWSMDRRMKKSWLRRALPILALLVAIDILVNVWQ
SYYDFRAINALAARLLDVQSSLMAGRIADQFRDVTALTSGTERLLDTGTLTDATLVNLTEALDNPLRRALIGVFDPQGEH
LLASSRPSFMNEVPPLDQILARVRANPSNAVILPVAWGERGAVVVAKGHQNRAIDLDAVVVIILTYERLVLDGVNLAPGT
SVLLLDSEHRLVMRDPEHPTLAVGQVLREDRGRAGPTTATQYASSQIDGIERLVSTRSIATRLTPDHWTVAVGYSVSDFR
EGFWRSTYINAAGAAVMLLMLVSGILLILHERRLQDRVERFASMVSTVVKNMPTPVAVVDHDTEQILLTNEALLADFGAV
AGAGQSFSRLFANAADWSQVCTTRMDEAIPMLTRDGTRYMLVHCAQLEFGGESVASKAVLVTLVDVNSQQQMLKELRTQA
DLDALTGLANRRYFENAAQKAVMHARKQGSALSILALDLDLFKRVNDTYGHAAGDRVLQVVARQFEGALRSTDLGARIGG
EEFTAILFDTALGQAQVIAERIRAAIQNTPIILESGEAITQTVSIGIAAYREDESDLAAAQERADAALYSAKAAGRNRVH
VYVPETSAPQDAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=37.5, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081881, Length=206, Percent_Identity=38.8349514563107, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=38.0434782608696, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=38.2857142857143, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=38.1818181818182, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=242, Percent_Identity=28.099173553719, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787541, Length=189, Percent_Identity=32.8042328042328, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788085, Length=196, Percent_Identity=30.1020408163265, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=39.3548387096774, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=32.0512820512821, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=35.1515151515151, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 71154; Mature: 71154

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSRDKSPLAWGKIRHVQVSEQVISDQSVDVSSAGIMGHSQSSATYWSMDRRMKKSWLR
CCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RALPILALLVAIDILVNVWQSYYDFRAINALAARLLDVQSSLMAGRIADQFRDVTALTSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TERLLDTGTLTDATLVNLTEALDNPLRRALIGVFDPQGEHLLASSRPSFMNEVPPLDQIL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCCCHHHHH
ARVRANPSNAVILPVAWGERGAVVVAKGHQNRAIDLDAVVVIILTYERLVLDGVNLAPGT
HHHHCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SVLLLDSEHRLVMRDPEHPTLAVGQVLREDRGRAGPTTATQYASSQIDGIERLVSTRSIA
EEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRLTPDHWTVAVGYSVSDFREGFWRSTYINAAGAAVMLLMLVSGILLILHERRLQDRVER
HCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FASMVSTVVKNMPTPVAVVDHDTEQILLTNEALLADFGAVAGAGQSFSRLFANAADWSQV
HHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
CTTRMDEAIPMLTRDGTRYMLVHCAQLEFGGESVASKAVLVTLVDVNSQQQMLKELRTQA
HHHHHHHHCCHHCCCCCEEEEEEEEHHCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHH
DLDALTGLANRRYFENAAQKAVMHARKQGSALSILALDLDLFKRVNDTYGHAAGDRVLQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHH
VARQFEGALRSTDLGARIGGEEFTAILFDTALGQAQVIAERIRAAIQNTPIILESGEAIT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCHHH
QTVSIGIAAYREDESDLAAAQERADAALYSAKAAGRNRVHVYVPETSAPQDAG
HHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MLTSRDKSPLAWGKIRHVQVSEQVISDQSVDVSSAGIMGHSQSSATYWSMDRRMKKSWLR
CCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RALPILALLVAIDILVNVWQSYYDFRAINALAARLLDVQSSLMAGRIADQFRDVTALTSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TERLLDTGTLTDATLVNLTEALDNPLRRALIGVFDPQGEHLLASSRPSFMNEVPPLDQIL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCCCHHHHH
ARVRANPSNAVILPVAWGERGAVVVAKGHQNRAIDLDAVVVIILTYERLVLDGVNLAPGT
HHHHCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SVLLLDSEHRLVMRDPEHPTLAVGQVLREDRGRAGPTTATQYASSQIDGIERLVSTRSIA
EEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRLTPDHWTVAVGYSVSDFREGFWRSTYINAAGAAVMLLMLVSGILLILHERRLQDRVER
HCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FASMVSTVVKNMPTPVAVVDHDTEQILLTNEALLADFGAVAGAGQSFSRLFANAADWSQV
HHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
CTTRMDEAIPMLTRDGTRYMLVHCAQLEFGGESVASKAVLVTLVDVNSQQQMLKELRTQA
HHHHHHHHCCHHCCCCCEEEEEEEEHHCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHH
DLDALTGLANRRYFENAAQKAVMHARKQGSALSILALDLDLFKRVNDTYGHAAGDRVLQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHH
VARQFEGALRSTDLGARIGGEEFTAILFDTALGQAQVIAERIRAAIQNTPIILESGEAIT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCHHH
QTVSIGIAAYREDESDLAAAQERADAALYSAKAAGRNRVHVYVPETSAPQDAG
HHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]