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The map label for this gene is ppiB [H]

Identifier: 94310027

GI number: 94310027

Start: 1174506

End: 1175006

Strand: Reverse

Name: ppiB [H]

Synonym: Rmet_1082

Alternate gene names: 94310027

Gene position: 1175006-1174506 (Counterclockwise)

Preceding gene: 94310028

Following gene: 94310026

Centisome position: 29.91

GC content: 60.88

Gene sequence:

>501_bases
ATGAGCAAGGTACAACTCCAAACCACGCAAGGCGCCATCGTCATCGAACTGGACGCCGAGAAGGCCCCGAAGTCGGTCGA
GAACTTCCTGTCGTACGTGCGCAAGGGCCACTATGACAACACGATCTTCCACCGCGTCATCAAGAACTTCATGATCCAGG
GCGGCGGCTTCGAGCCGGGCATGAAGCAGAAGGACACCGATGCGCCGATCGAGAACGAAGCCGGCAACGGCCTGAAGAAC
GACCGCTACACGGTTGCCATGGCCCGCACCAACGCGCCGCACTCGGCCACGGCCCAGTTCTTCATCAACGTGGTGGACAA
CGACTTCCTGAACTTCAGCTCGCCCACGCCGCAAGGCTTCGGCTATGCGGTGTTCGGCAAGGTCGTGGAAGGCACCGACG
TGATCGACGCCATCAAAGGCGTGCGCACCGGCAGCTCGGGCTTCCATCAGGACGTGCCGCTCGAGGACGTCGTGATCGAG
AAAGCCACGATTGTCGAGTAA

Upstream 100 bases:

>100_bases
TGCCGCGCCGATCGTGATCGAATCGGCCACCGTGGTCACCAAGTGACCCCAGGCAACCCCGAATACAACCGTCATCGCAT
CATCGAACAGAGGTAACCCT

Downstream 100 bases:

>100_bases
TCGCGCTTAACGTCGATTCGAACGACAGGCGGCATGCCGCTCGTGGTGAATACAGACATCCGGACCGCATGACCGCTCCC
ACCCGCACGCCGGTGGCCGC

Product: peptidyl-prolyl cis-trans isomerase B

Products: NA

Alternate protein names: PPIase cyp18; SanCyp18; Rotamase cyp18 [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSKVQLQTTQGAIVIELDAEKAPKSVENFLSYVRKGHYDNTIFHRVIKNFMIQGGGFEPGMKQKDTDAPIENEAGNGLKN
DRYTVAMARTNAPHSATAQFFINVVDNDFLNFSSPTPQGFGYAVFGKVVEGTDVIDAIKGVRTGSSGFHQDVPLEDVVIE
KATIVE

Sequences:

>Translated_166_residues
MSKVQLQTTQGAIVIELDAEKAPKSVENFLSYVRKGHYDNTIFHRVIKNFMIQGGGFEPGMKQKDTDAPIENEAGNGLKN
DRYTVAMARTNAPHSATAQFFINVVDNDFLNFSSPTPQGFGYAVFGKVVEGTDVIDAIKGVRTGSSGFHQDVPLEDVVIE
KATIVE
>Mature_165_residues
SKVQLQTTQGAIVIELDAEKAPKSVENFLSYVRKGHYDNTIFHRVIKNFMIQGGGFEPGMKQKDTDAPIENEAGNGLKND
RYTVAMARTNAPHSATAQFFINVVDNDFLNFSSPTPQGFGYAVFGKVVEGTDVIDAIKGVRTGSSGFHQDVPLEDVVIEK
ATIVE

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7706339, Length=163, Percent_Identity=34.3558282208589, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI19557636, Length=166, Percent_Identity=34.3373493975904, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI24308049, Length=164, Percent_Identity=34.7560975609756, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI22547215, Length=164, Percent_Identity=32.3170731707317, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI22547212, Length=164, Percent_Identity=32.3170731707317, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI7657473, Length=164, Percent_Identity=32.3170731707317, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI64276486, Length=131, Percent_Identity=38.9312977099237, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI4758950, Length=162, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=68.7116564417178, Blast_Score=231, Evalue=2e-62,
Organism=Escherichia coli, GI1789763, Length=162, Percent_Identity=56.1728395061728, Blast_Score=169, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71980590, Length=144, Percent_Identity=38.1944444444444, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71980594, Length=144, Percent_Identity=38.1944444444444, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17532641, Length=164, Percent_Identity=33.5365853658537, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17506311, Length=143, Percent_Identity=34.2657342657343, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552780, Length=158, Percent_Identity=34.1772151898734, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539496, Length=162, Percent_Identity=30.8641975308642, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320510, Length=156, Percent_Identity=33.974358974359, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17986117, Length=166, Percent_Identity=31.3253012048193, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922376, Length=170, Percent_Identity=28.8235294117647, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922912, Length=160, Percent_Identity=35, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652460, Length=164, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18155; Mature: 18023

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVQLQTTQGAIVIELDAEKAPKSVENFLSYVRKGHYDNTIFHRVIKNFMIQGGGFEPG
CCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
MKQKDTDAPIENEAGNGLKNDRYTVAMARTNAPHSATAQFFINVVDNDFLNFSSPTPQGF
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEEHHCCCCCCCCCCCCCCC
GYAVFGKVVEGTDVIDAIKGVRTGSSGFHQDVPLEDVVIEKATIVE
CHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCC
>Mature Secondary Structure 
SKVQLQTTQGAIVIELDAEKAPKSVENFLSYVRKGHYDNTIFHRVIKNFMIQGGGFEPG
CCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
MKQKDTDAPIENEAGNGLKNDRYTVAMARTNAPHSATAQFFINVVDNDFLNFSSPTPQGF
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHEEHHCCCCCCCCCCCCCCC
GYAVFGKVVEGTDVIDAIKGVRTGSSGFHQDVPLEDVVIEKATIVE
CHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10400660 [H]