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The map label for this gene is trmJ [H]

Identifier: 94310024

GI number: 94310024

Start: 1171656

End: 1172525

Strand: Direct

Name: trmJ [H]

Synonym: Rmet_1079

Alternate gene names: 94310024

Gene position: 1171656-1172525 (Clockwise)

Preceding gene: 94310018

Following gene: 94310025

Centisome position: 29.83

GC content: 66.9

Gene sequence:

>870_bases
ATGACCGAAGTGTTGAACTCTCAGATGGATCCGATCCCGCGCGGCGCGGCCGCAAGTGATCTTTCGGACATTTTTGCGCA
TGTGCGTGTGGTGCTGGTGGAAACCAGCCACCCCGGCAACGTGGGCTCGGTCGCGCGGGCGATCAAGACGATGGGCTTCG
GGCACGTGCCGGGGAGCCTGGTGCTGGTCTCGCCGCGCGAGCCGGGCGTCGTGTCGCATCCCGATGCCGTCGCGATGGCC
AGTGGTGCCGACGATGTGCTGGCCAGCGCCACGATCGTCGACAGCATCGAGGCCGCGCTGGCTGGCACGGCCTTTACCGT
CGCCATGACCGCCCGGCAGCGCGAATTCGGGCCGCCGAGGCTGCTACCGCGCGCCGCCGTGGCCAAGGCGCGGTCGGTGC
TGGAGGCGGACCCCGCTACCACGGTCGCCTTCGTATTCGGCAATGAACGCTTCGGCTTGCCGAACGACGCGGTATTGCGC
TGTTCGGCGGTGACGCATATTCCTGCCAATCCGGTTTACGCCTCTCTCAACCTGGCCCAGGCCGTGCAGCTCATTGCCTA
TGAGATGCGGCTGGCCCTGCTCGAGGCGCAGGAAACGGCCGGCGGGCTCCCGGACGTCGCTGGCGATATCGGCTATGCTG
GTGACCCCGCCACCACCGAGCAGATCGAGGCGATGTTTGGCCACCTTCAGGCCGGGCTTGCGGCGATCGGCTTTCTGGAC
CCGGCGAATCCGCGCAAGCTGATGCCGCGCCTGCGCCGGTTGTTCGCGCGGACGGGCCTGGAGCGCGAGGAAGTGAACAT
CCTGCGTGGCATCGCCAAGCGCATGCTGCTGACGGGCGATGCAAAGCACGACGACAACAAGGAAGACTAG

Upstream 100 bases:

>100_bases
CATGTGAGGCGCCGCACCGCAATTGGAAGGCAGCATTGTATAGGAACCTGGCCTCTCGCACGAGTCGACAACCTCGATCG
AGACGGCCGGGACGCTCCGG

Downstream 100 bases:

>100_bases
TTCCGTGGCGATTTTCCCGGCGTACATCATGCAAGACCTCATCCGTGGCGCGGTGTCCGGTCGCTCGGTGGTGAGGCATG
CGTGCGCGCATTTGCCGGAA

Product: putative RNA methyltransferase (TrmH family)

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSLVLVSPREPGVVSHPDAVAMA
SGADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPRLLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLR
CSAVTHIPANPVYASLNLAQAVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLD
PANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED

Sequences:

>Translated_289_residues
MTEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSLVLVSPREPGVVSHPDAVAMA
SGADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPRLLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLR
CSAVTHIPANPVYASLNLAQAVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLD
PANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED
>Mature_288_residues
TEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSLVLVSPREPGVVSHPDAVAMAS
GADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPRLLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLRC
SAVTHIPANPVYASLNLAQAVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLDP
ANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=251, Percent_Identity=45.0199203187251, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1790865, Length=171, Percent_Identity=30.4093567251462, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30442; Mature: 30311

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSL
CCCHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCE
VLVSPREPGVVSHPDAVAMASGADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPR
EEECCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
LLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLRCSAVTHIPANPVYASLNLAQ
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEHEEECCCCCCEEHHHHHH
AVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLD
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSL
CCHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCE
VLVSPREPGVVSHPDAVAMASGADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPR
EEECCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
LLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLRCSAVTHIPANPVYASLNLAQ
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEHEEECCCCCCEEHHHHHH
AVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLD
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]