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The map label for this gene is pyrG [H]

Identifier: 94309997

GI number: 94309997

Start: 1145343

End: 1146992

Strand: Direct

Name: pyrG [H]

Synonym: Rmet_1052

Alternate gene names: 94309997

Gene position: 1145343-1146992 (Clockwise)

Preceding gene: 94309996

Following gene: 94309998

Centisome position: 29.16

GC content: 62.85

Gene sequence:

>1650_bases
ATGACCAAATTCGTCTTCGTCACCGGTGGCGTCGTCTCTTCTCTCGGCAAGGGCATCGCTGCTGCTTCGCTCGCGGCTAT
TCTCGAGTCGCGCGGCCTCAAAGTCACCCTCCTCAAGCTCGATCCCTACATCAACGTCGATCCCGGCACGATGAGCCCCT
TCCAGCATGGCGAGGTGTTCGTGACCGAAGATGGCGCGGAAACCGACCTCGACCTGGGGCACTACGAGCGGTTCGTGTCG
GCCAAGATGCGGAAGTCGAACAACTTCACGACTGGCCAGATCTACGAATCGGTGATCCGCAAGGAACGCCGCGGTGAGTA
CCTCGGCAAGACCGTTCAGGTCATTCCCCATATCACGAACGAAATCCAGGCTTTCGTCGAGCGTGGCGCTGCCGCGTCGC
ACGACGGCAAGGCCGACGTGGCACTGGTGGAGATCGGCGGCACGGTGGGTGACATCGAGTCGCTGCCGTTCCTGGAAGCC
GCGCGCCAGATGAGCCTGCGCCTTGGCCGCAATCACTGCGCGTTCGTGCACCTGACGCTGGTGCCGTTCATCGCCAGCGC
CGGCGAGCTCAAGACCAAGCCGACCCAGCACTCGGTGCAGAAGCTCCGCGAAATCGGCATTTCGCCGACCGCGCTGCTGT
GCCGCGCCGACCGCCCGATTCCGGACGACGAGCGGGCCAAGATTTCGCTGTTCGCCAACATCCCGCAGGAAGCCGTGATC
TCGGTCTGGGATGCCGACAGCATCTACAAGATTCCGCAGATGCTCAACGAGCAGGGCCTCGACCGCCTGATCTGCGAGGA
ACTGCGTCTGGACCCGAAGCCGGCTGACCTGTCGATGTGGCAGAAGCTGGTCAAGGCACAGGAAAACCCCGAGCACGAGA
TCACGATCGGCATGGTTGGCAAGTACGTCGACCTGACCGAGTCGTACAAGTCACTGATCGAGGCGCTGCGCCACGCTGGC
ATGCATACCGCCACGCGCGTGAACATCGAGTACATCGATTCGGAAGAACTCGAGTCGGGTCACCTCGAAGTGCTGCAGCC
GCTGGATGCCATCCTGGTACCGGGCGGTTTCGGCAAGCGCGGTACCGAAGGCAAGATCCGCGCGATCCAGTATGCCCGCG
AGAACAAGGTGCCGTACCTGGGCATCTGCCTGGGTATGCAGCTCGCCGTGATCGAATTCGCGCGTCACGTTGCCAGCATG
GGCGACGCCAATTCGACCGAATTCAACCTCGACACGGAACACCCGGTAGTCGCGCTGATCACCGAGTGGGTCGATCGCGA
CGGCAAGGTGGAACAGCGTTCGGCAGACTCCGATCTCGGCGGCACGATGCGCCTGGGCGCCCAGCGGGTACCCATCAAGA
CCGGCACCAAGGCCTCGCAGATCTATGGCGCCGAAGTCAACGAGCGTCATCGTCACCGCTACGAAGTCAACAACCACTAC
GTGCCGGAGCTGGAAAAGGCTGGCATGGTGATCTCGGCCCGCACGCCGACCGAGAACCTGCCGGAGATGATGGAACTGCC
GGAATCGATGCACCCGTGGTTCGTCGGCGTTCAGTTCCACCCGGAATTCACCTCGACGCCGCGTGATGGCCACCCGCTGT
TCAAGGCCTACGTGGAAGCCGCGCTCGCCGCGCAACGCCAAGGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCATTGCGTGCCGGGCCGACACCTCGATTTCACAAATTCGGCGTCGGCGGGGATCGGGCTCTCATGTATAATCCCGAT
TTCCCCGGCAAGCTCGGTTT

Downstream 100 bases:

>100_bases
GGCGCTGGTGGGGAAGGAAACTGCCATGAAACTGTGTGGATTCGACGTCGGCCTCGACAAGCCGTTTTTCCTGATCGCTG
GTCCGTGCGTGATCGAGTCC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFVERGAAASHDGKADVALVEIGGTVGDIESLPFLEA
ARQMSLRLGRNHCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQEAVI
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVKAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAG
MHTATRVNIEYIDSEELESGHLEVLQPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVASM
GDANSTEFNLDTEHPVVALITEWVDRDGKVEQRSADSDLGGTMRLGAQRVPIKTGTKASQIYGAEVNERHRHRYEVNNHY
VPELEKAGMVISARTPTENLPEMMELPESMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAAQRQGA

Sequences:

>Translated_549_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFVERGAAASHDGKADVALVEIGGTVGDIESLPFLEA
ARQMSLRLGRNHCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQEAVI
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVKAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAG
MHTATRVNIEYIDSEELESGHLEVLQPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVASM
GDANSTEFNLDTEHPVVALITEWVDRDGKVEQRSADSDLGGTMRLGAQRVPIKTGTKASQIYGAEVNERHRHRYEVNNHY
VPELEKAGMVISARTPTENLPEMMELPESMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAAQRQGA
>Mature_548_residues
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVSA
KMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFVERGAAASHDGKADVALVEIGGTVGDIESLPFLEAA
RQMSLRLGRNHCAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQEAVIS
VWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVKAQENPEHEITIGMVGKYVDLTESYKSLIEALRHAGM
HTATRVNIEYIDSEELESGHLEVLQPLDAILVPGGFGKRGTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVASMG
DANSTEFNLDTEHPVVALITEWVDRDGKVEQRSADSDLGGTMRLGAQRVPIKTGTKASQIYGAEVNERHRHRYEVNNHYV
PELEKAGMVISARTPTENLPEMMELPESMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAAQRQGA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=564, Percent_Identity=45.5673758865248, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=44.3649373881932, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=44.3649373881932, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=44.3649373881932, Blast_Score=472, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=549, Percent_Identity=62.4772313296903, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=616, Percent_Identity=38.6363636363636, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.2280701754386, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322563, Length=574, Percent_Identity=41.2891986062718, Blast_Score=433, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=45, Blast_Score=470, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=43.7623762376238, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60705; Mature: 60574

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFVERGAAASHDGKADVALVEIGGTVGDIESLPFLEAARQMSLRLGRNHCAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHH
VPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQEAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHE
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVKAQENPEHEITIGMVG
EECCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGMHTATRVNIEYIDSEELESGHLEVLQPLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCCCCHHHHCCHHHEEECCCCCCC
GTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVASMGDANSTEFNLDTEHPVVALI
CCCCCCCEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
TEWVDRDGKVEQRSADSDLGGTMRLGAQRVPIKTGTKASQIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCHHCCCCCCCCCHHHCCCEECCCCCCCCHHHEECCCCCHHHHHHCCCCCCC
VPELEKAGMVISARTPTENLPEMMELPESMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCHHHCCEEEEECCCCCCCHHHHHCCHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAAQRQGA
HHHHHHCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKSNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFVERGAAASHDGKADVALVEIGGTVGDIESLPFLEAARQMSLRLGRNHCAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHH
VPFIASAGELKTKPTQHSVQKLREIGISPTALLCRADRPIPDDERAKISLFANIPQEAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHE
SVWDADSIYKIPQMLNEQGLDRLICEELRLDPKPADLSMWQKLVKAQENPEHEITIGMVG
EECCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGMHTATRVNIEYIDSEELESGHLEVLQPLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCCCCHHHHCCHHHEEECCCCCCC
GTEGKIRAIQYARENKVPYLGICLGMQLAVIEFARHVASMGDANSTEFNLDTEHPVVALI
CCCCCCCEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
TEWVDRDGKVEQRSADSDLGGTMRLGAQRVPIKTGTKASQIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCHHCCCCCCCCCHHHCCCEECCCCCCCCHHHEECCCCCHHHHHHCCCCCCC
VPELEKAGMVISARTPTENLPEMMELPESMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCHHHCCEEEEECCCCCCCHHHHHCCHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAAQRQGA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]