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The map label for this gene is nth [H]

Identifier: 94309959

GI number: 94309959

Start: 1103987

End: 1104631

Strand: Reverse

Name: nth [H]

Synonym: Rmet_1014

Alternate gene names: 94309959

Gene position: 1104631-1103987 (Counterclockwise)

Preceding gene: 94309960

Following gene: 94309957

Centisome position: 28.12

GC content: 62.79

Gene sequence:

>645_bases
ATGAACTCCGCCAAGTGCCGCGCCATCTTCGAAACCCTGCGTGAGGTCAATCCGACGCCGACCACGGAGCTGGAGTACAG
CTCGCCGTTCGAATTGCTGATCGCGGTGCTGCTCTCCGCGCAGGCCACCGATGTCGGCGTCAACAAGGCCACGCGCCTGC
TGTTCCCGGTTGCGCATTCGCCGCAGCAGATCCTGGCGCTTGGCGAGGCGGGCCTGATCGACTACATCAAGACGATCGGC
CTCTACAAGACCAAGGCCAAGCACGTAATGGAAACGTGCCGCATTCTTGTGGAGAAGTACGACGGCAAGGTGCCGCCGGT
GCGCGAGGCGCTGGAGTCGCTGCCCGGCGTCGGCCGCAAGACGGCAAACGTGGTGCTCAACGTTGCGTTCGGCCAGCCGA
CGATCGCCGTGGACACGCACATTTTCCGCGTATCGAACCGTACTGGCCTGGCGCCCGGCAAGAATCCCGATGCAGTGGAG
CAGAAGCTGCTGAAGGTCGTGCCGAAAGAATTCCTGCACGATGCGCACCACTGGCTGATCCTGCATGGTCGCTACGTGTG
CAAGGCGCGCAAGCCCGAGTGCTGGCACTGCGCGATCGAACCCCTCTGCGAGTTCAAGGAAAAAACGCCCGCGCCCAAGC
AGTAA

Upstream 100 bases:

>100_bases
CCGAAGCACGGCGGGCAAAGCTTGGCATGACCACGCACGAACCGGCCGGCGAGCAGGGCAACAAACAGGAAAACAAGCAG
GACAACAACAACCCTTCGAC

Downstream 100 bases:

>100_bases
TCGGTATCCAGTCGCCGGTCGTCAGGAACGCTGACGATCCTTCAGCACCAGCGCCAGCTGCAGCGCCGTCGCCGCAACGG
TATCGACATCGGGCGCATAG

Product: DNA glycosylase and apyrimidinic (AP) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHSPQQILALGEAGLIDYIKTIG
LYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRKTANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVE
QKLLKVVPKEFLHDAHHWLILHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ

Sequences:

>Translated_214_residues
MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHSPQQILALGEAGLIDYIKTIG
LYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRKTANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVE
QKLLKVVPKEFLHDAHHWLILHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ
>Mature_214_residues
MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHSPQQILALGEAGLIDYIKTIG
LYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRKTANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVE
QKLLKVVPKEFLHDAHHWLILHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI115298648, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI190358497, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI6912520, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI115298654, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI115298652, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI115298650, Length=138, Percent_Identity=32.6086956521739, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=69.7115384615385, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=33.8983050847458, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=28.1609195402299, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.668449197861, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=32.0855614973262, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23767; Mature: 23767

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHEECCCCC
PQQILALGEAGLIDYIKTIGLYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVEQKLLKVVPKEFLHDAHHWLI
CEEEEEEEECCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEE
LHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ
EECEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHEECCCCC
PQQILALGEAGLIDYIKTIGLYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVEQKLLKVVPKEFLHDAHHWLI
CEEEEEEEECCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEE
LHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ
EECEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]