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The map label for this gene is grpE

Identifier: 94309949

GI number: 94309949

Start: 1095267

End: 1095809

Strand: Direct

Name: grpE

Synonym: Rmet_1004

Alternate gene names: 94309949

Gene position: 1095267-1095809 (Clockwise)

Preceding gene: 94309947

Following gene: 94309950

Centisome position: 27.88

GC content: 66.67

Gene sequence:

>543_bases
ATGGAAGAACAGAACCAGACGCCAACCCCCAATACGCAGGCAGCCGAGGACGCCGCCCGCACGCCGGAAGCCGGTGCCGA
CGAGGTGGGCCGCCTGACGCAACAGGTGGCCGAACTGGAAGCCAAGGCGAAGGAACACTACGACATGTTCCTGCGCGCCA
CGGCGGAGGGCGAGAACATCCGCCGCCGTTCGCAGGACGAAGTGGCCAAGGCCCACAAGTTCGCCATCGAGAGCTTTGCC
GATAACCTCGTGCCCGTGATGGACAGCCTGCAGGCCGCGCTGGCCGACGGTACCGGTGACCTCGGCAAGCTGCGCGAAGG
TGTGGAACTGACCGCACGCCAGCTGGCCGCCGCGTTCGAACGTGGTCGCATCGTCGAAGTGAACCCGGTGGGCGAGAAGT
TCGATCCGCATCGCCACCAGGCCATCTCGATGGTGCCGTCCGAGCAGGAACCGAACACCGTGGTCAACGTGCTGCAACGC
GGCTACATGATTGCCGACCGCGTGCTGCGCCCGGCGCTGGTGACCGTCTCGGCGCCGCGCTGA

Upstream 100 bases:

>100_bases
GACTGACACCAACTGCGGCTAGACTTGCCGGTTTGACACCCGACTCACCCGATGGCCTGCCGCCAGAGCGGGCCGTGCTT
TTGTTTTGATACGAATCGAC

Downstream 100 bases:

>100_bases
GCGCGAGTCGTCCGCATACGGCAGTACGAAACGCCCGGGCCATTGGCGCCGGGCGTTGTCGTTTCAGGAGCGATCGAAAT
GTCTGAAGAAAACCAGCCGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MEEQNQTPTPNTQAAEDAARTPEAGADEVGRLTQQVAELEAKAKEHYDMFLRATAEGENIRRRSQDEVAKAHKFAIESFA
DNLVPVMDSLQAALADGTGDLGKLREGVELTARQLAAAFERGRIVEVNPVGEKFDPHRHQAISMVPSEQEPNTVVNVLQR
GYMIADRVLRPALVTVSAPR

Sequences:

>Translated_180_residues
MEEQNQTPTPNTQAAEDAARTPEAGADEVGRLTQQVAELEAKAKEHYDMFLRATAEGENIRRRSQDEVAKAHKFAIESFA
DNLVPVMDSLQAALADGTGDLGKLREGVELTARQLAAAFERGRIVEVNPVGEKFDPHRHQAISMVPSEQEPNTVVNVLQR
GYMIADRVLRPALVTVSAPR
>Mature_180_residues
MEEQNQTPTPNTQAAEDAARTPEAGADEVGRLTQQVAELEAKAKEHYDMFLRATAEGENIRRRSQDEVAKAHKFAIESFA
DNLVPVMDSLQAALADGTGDLGKLREGVELTARQLAAAFERGRIVEVNPVGEKFDPHRHQAISMVPSEQEPNTVVNVLQR
GYMIADRVLRPALVTVSAPR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=154, Percent_Identity=37.6623376623377, Blast_Score=100, Evalue=6e-22,
Organism=Homo sapiens, GI40255109, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1788967, Length=186, Percent_Identity=38.1720430107527, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=174, Percent_Identity=34.4827586206897, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=157, Percent_Identity=33.1210191082803, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=37.3417721518987, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_RALME (Q1LPN6)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583159.1
- ProteinModelPortal:   Q1LPN6
- SMR:   Q1LPN6
- STRING:   Q1LPN6
- GeneID:   4037801
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1004
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q1LPN6
- ProtClustDB:   PRK14139
- BioCyc:   RMET266264:RMET_1004-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19755; Mature: 19755

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQNQTPTPNTQAAEDAARTPEAGADEVGRLTQQVAELEAKAKEHYDMFLRATAEGENI
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRRSQDEVAKAHKFAIESFADNLVPVMDSLQAALADGTGDLGKLREGVELTARQLAAAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RGRIVEVNPVGEKFDPHRHQAISMVPSEQEPNTVVNVLQRGYMIADRVLRPALVTVSAPR
CCCEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MEEQNQTPTPNTQAAEDAARTPEAGADEVGRLTQQVAELEAKAKEHYDMFLRATAEGENI
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRRSQDEVAKAHKFAIESFADNLVPVMDSLQAALADGTGDLGKLREGVELTARQLAAAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RGRIVEVNPVGEKFDPHRHQAISMVPSEQEPNTVVNVLQRGYMIADRVLRPALVTVSAPR
CCCEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA