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The map label for this gene is nuoI [H]

Identifier: 94309880

GI number: 94309880

Start: 1020809

End: 1021300

Strand: Direct

Name: nuoI [H]

Synonym: Rmet_0935

Alternate gene names: 94309880

Gene position: 1020809-1021300 (Clockwise)

Preceding gene: 94309879

Following gene: 94309881

Centisome position: 25.99

GC content: 60.16

Gene sequence:

>492_bases
ATGCTGCTCGCCATCAAGGACTTCTTCAACAGCCTGCTCCTGAAGGAACTCTTCAAGGGTATGGCTTTGACCGGCCGCTA
TCTCTTCGCGCGCAAGATCACAGTCCAGTTCCCGGAAGAGAAGACGCCGATGTCGCCGCGTTTCCGCGGCCTGCACGCAC
TGCGCCGCTATCCCAATGGGGAAGAGCGTTGCATCGCCTGCAAGCTGTGTGAAGCGGTGTGCCCGGCATTGGCTATCTCG
ATCGAATCGGACGTGCGTAACGACGGCACCCGCCGCACCACGCGCTACGACATCGACCTGACCAAGTGCATTTTCTGCGG
CTTCTGCGAAGAAGCTTGCCCGGTTGACGCGATCGTCGAGACGCACATTCTCGAGTATCACGGCGAAAAGCGCGGTGACC
TGTACTTCACCAAGGACATGCTGCTGGCCGTTGGCGACCGCTACGAGCCGCAGATCGCCGCAGCCAAGGCTGCGGACGCG
AAGTACCGTTAA

Upstream 100 bases:

>100_bases
TCAAGTCGCCGTGGAACATCTGGCACTAAGAGGCCAGGCGATGGTGCGTCACTGCCAGAGGCAGGGCGCGCCGCGCGGAA
ATACTTAGGAAGCAATCGTC

Downstream 100 bases:

>100_bases
GCGTATCGACGCAGGGCGGGAGCAGGGCGCTTGAGGCGCCGGCTCCCGAAAGCGAAATTGAATCGGTCCCGCAAGGGGCC
AACAGAAGGATGCCGCGAGC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MLLAIKDFFNSLLLKELFKGMALTGRYLFARKITVQFPEEKTPMSPRFRGLHALRRYPNGEERCIACKLCEAVCPALAIS
IESDVRNDGTRRTTRYDIDLTKCIFCGFCEEACPVDAIVETHILEYHGEKRGDLYFTKDMLLAVGDRYEPQIAAAKAADA
KYR

Sequences:

>Translated_163_residues
MLLAIKDFFNSLLLKELFKGMALTGRYLFARKITVQFPEEKTPMSPRFRGLHALRRYPNGEERCIACKLCEAVCPALAIS
IESDVRNDGTRRTTRYDIDLTKCIFCGFCEEACPVDAIVETHILEYHGEKRGDLYFTKDMLLAVGDRYEPQIAAAKAADA
KYR
>Mature_163_residues
MLLAIKDFFNSLLLKELFKGMALTGRYLFARKITVQFPEEKTPMSPRFRGLHALRRYPNGEERCIACKLCEAVCPALAIS
IESDVRNDGTRRTTRYDIDLTKCIFCGFCEEACPVDAIVETHILEYHGEKRGDLYFTKDMLLAVGDRYEPQIAAAKAADA
KYR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=153, Percent_Identity=61.437908496732, Blast_Score=201, Evalue=3e-52,
Organism=Escherichia coli, GI1788617, Length=161, Percent_Identity=39.1304347826087, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17555194, Length=155, Percent_Identity=56.1290322580645, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17864306, Length=154, Percent_Identity=57.7922077922078, Blast_Score=194, Evalue=3e-50,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18581; Mature: 18581

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAIKDFFNSLLLKELFKGMALTGRYLFARKITVQFPEEKTPMSPRFRGLHALRRYPNG
CEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCC
EERCIACKLCEAVCPALAISIESDVRNDGTRRTTRYDIDLTKCIFCGFCEEACPVDAIVE
CHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHH
THILEYHGEKRGDLYFTKDMLLAVGDRYEPQIAAAKAADAKYR
HHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MLLAIKDFFNSLLLKELFKGMALTGRYLFARKITVQFPEEKTPMSPRFRGLHALRRYPNG
CEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCC
EERCIACKLCEAVCPALAISIESDVRNDGTRRTTRYDIDLTKCIFCGFCEEACPVDAIVE
CHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHH
THILEYHGEKRGDLYFTKDMLLAVGDRYEPQIAAAKAADAKYR
HHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA