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The map label for this gene is nuoB

Identifier: 94309873

GI number: 94309873

Start: 1012913

End: 1013395

Strand: Direct

Name: nuoB

Synonym: Rmet_0928

Alternate gene names: 94309873

Gene position: 1012913-1013395 (Clockwise)

Preceding gene: 94309872

Following gene: 94309874

Centisome position: 25.79

GC content: 61.49

Gene sequence:

>483_bases
ATGGCAATCGAAGGCGTTCTCAACGAAGGCTTTGTCACGACGACCGCTGACAAGCTGATCAATTGGACCCGTACGGGTTC
CCTGTGGCCCATGACGTTCGGTCTGGCCTGCTGTGCAGTGGAAATGATGCACGCCGGCGCCGCGCGTTACGACATGGACC
GTTTCGGTGTGATTTTCCGCCCGTCGCCGCGTCAGTCCGACGTGATGATCGTGGCAGGCACGCTGTGCAACAAGATGGCC
CCCGCGCTGCGCAAGGTGTACGACCAGATGGCCGAACCGCGCTGGGTGATCTCGATGGGGTCGTGCGCCAACGGCGGCGG
CTACTATCACTACTCATATTCGGTGGTGCGTGGCTGCGACCGCATCGTGCCGGTCGACATCTATGTTCCGGGCTGCCCGC
CGACGGCGGAAGCGCTGATCTACGGCGTGATCCAGCTCCAGAACAAGATCAAGCGTACCAATACTATCGCGCGCAAGGGC
TGA

Upstream 100 bases:

>100_bases
TTGGCCGGGATTCATCGCCATGGGCGTGTTTCTGCTGGAATTCATCGTGGGCTTCGTCTACATCTGGAAAAAGGGCGCGC
TCGATTGGGAGTGATGTGAA

Downstream 100 bases:

>100_bases
TATGGCGAACCTGGATACCCTCAAGGCTGCGCTCGAAAAGGTCCTCGGCAAGCGCGTGCAGAACCTGATCGAGGCCACCG
GCGAACTGACGCTGATCGTC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG

Sequences:

>Translated_160_residues
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
>Mature_159_residues
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=80.8219178082192, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=49.0066225165563, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=36.6906474820144, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.0243902439024, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=136, Percent_Identity=75.7352941176471, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=82.8571428571429, Blast_Score=262, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=82.8571428571429, Blast_Score=262, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=82.1428571428571, Blast_Score=256, Evalue=6e-69,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_CUPNH (Q0KCS9)

Other databases:

- EMBL:   AM260479
- RefSeq:   YP_725560.1
- ProteinModelPortal:   Q0KCS9
- SMR:   Q0KCS9
- STRING:   Q0KCS9
- GeneID:   4250212
- GenomeReviews:   AM260479_GR
- KEGG:   reh:H16_A1051
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q0KCS9
- ProtClustDB:   PRK06411
- BioCyc:   REUT381666:H16_A1051-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17635; Mature: 17504

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFR
CCCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFR
CCCCCCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA