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The map label for this gene is hemN [H]

Identifier: 94309795

GI number: 94309795

Start: 930787

End: 932046

Strand: Reverse

Name: hemN [H]

Synonym: Rmet_0850

Alternate gene names: 94309795

Gene position: 932046-930787 (Counterclockwise)

Preceding gene: 94309796

Following gene: 94309793

Centisome position: 23.73

GC content: 63.65

Gene sequence:

>1260_bases
ATGATCCCGATCGTCCCTGTCGGCGGTGGCACGCCGGCCACATCCTCGCCGCTATCCCCTGCCGACAACCAGAAGCTTTG
GCTCAAGCCGGGCCAGATCGCCCTGCCCGGCTCGCCGCCGCTTTCTCTCTACGTGCACATCCCATGGTGCGTGCGCAAAT
GCCCGTACTGTGATTTCAACTCGCACGCGGCACCGGGAAAGCCGGATAGCCACGAGATTCCCGAGGACGCCTACCTCGAC
GCGCTACGCGCAGATCTTGAGCAATCGCTGCCACTGGTATGGGGACGTCCCGTGCATACGGTGTTTATCGGCGGCGGCAC
ACCGAGCCTGCTGTCGGCCGCTGGCATGGACCGCCTGCTATCGGACATCCGCGCGCTGCTACCGCTCGATGCCGATGCCG
AGATTACGATGGAAGCCAACCCCGGCACCTTCGAAGCCGAACGGTTCGCAAGCTATCGCGCCAGCGGCATCAACCGGCTG
TCGATCGGCATCCAGAGTTTCAACGACGCCCACTTGAAAGCGCTTGGCCGGATCCACGGCGAGCGCGAGGCACGCGCGGC
CATCGACATCGCGCTGAAGCACTTCGACAACGTCAATCTGGACTTGATGTATGCGCTGCCGGGGCAGACCCTGGACGAAT
GCGCGCGCGACCTGGACGCAGCGTTGTCGTACGGCACCACGCACCTGTCGCTCTATCACCTGACGCTGGAACCGAACACG
CTGTTTGCGAAGTTTCCGCCCGCGCTGCCGGACGAGGACACCGCCTACGAGATGCAGGACATCGTCGAGGCCCGTACGGC
TCAGGCTGGGTATCGCCACTACGAGACATCGGCCTATGCGAAGCCGCATCGCGAGGCGCGCCACAACCTCAACTACTGGC
GCTTCGGCGACTACCTCGGCATCGGCGCCGGCGCCCACGGCAAGCTTTCGTTTCCAAACCGGATCCTGCGGCAGATGCGA
CACAAGCACCCGGCCACTTATATGGAGCAGGCCCTGGCTGGCAACGCCGTGCAGGAAGCGCGCGAAGTCGGCGCTGACGA
ACTCCCGTTCGAATTCATGCTCAACGCGCTGCGTCTGACGGACGGCGTGCCGGCATCGAGTTTCCACGACTACACCGGCC
TGCCATTGCACATGATCGGCAAGCAGCTTGCCGCAGCCGAGAAGAAGGGACTGCTCGACGCCGACCCTACCGTGATCAAG
CCGACTGAACTCGGCCGACGCTTCCTCAACGACCTGCAGGAGATGTTCCTGAAGGATTGA

Upstream 100 bases:

>100_bases
CCGAGCTGAGCGCCGAAGAGAAGAACGCGATCAGCCATCGTGCCCTGGCACTGCAGGAACTGGTGGCCCGCTTGCAGACC
GCCGGCATCAAGAGCTGCGC

Downstream 100 bases:

>100_bases
GGTTGCCCACACGGACGACGCAACGAGAGCGTCGAGCCAGAATGAAAAACGCCACGCGATTGCCGCGTGGCGTTTTCTTT
TGCCGATGTATCGAGATATC

Product: coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFNSHAAPGKPDSHEIPEDAYLD
ALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLLSDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRL
SIGIQSFNDAHLKALGRIHGEREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNT
LFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLGIGAGAHGKLSFPNRILRQMR
HKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLTDGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIK
PTELGRRFLNDLQEMFLKD

Sequences:

>Translated_419_residues
MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFNSHAAPGKPDSHEIPEDAYLD
ALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLLSDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRL
SIGIQSFNDAHLKALGRIHGEREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNT
LFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLGIGAGAHGKLSFPNRILRQMR
HKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLTDGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIK
PTELGRRFLNDLQEMFLKD
>Mature_419_residues
MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFNSHAAPGKPDSHEIPEDAYLD
ALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLLSDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRL
SIGIQSFNDAHLKALGRIHGEREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNT
LFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLGIGAGAHGKLSFPNRILRQMR
HKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLTDGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIK
PTELGRRFLNDLQEMFLKD

Specific function: Unknown

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=274, Percent_Identity=38.6861313868613, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1789325, Length=386, Percent_Identity=51.5544041450777, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI87082341, Length=401, Percent_Identity=28.927680798005, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 46105; Mature: 46105

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFN
CCEEEECCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCCEEEEEECCHHHHHCCCCCCC
SHAAPGKPDSHEIPEDAYLDALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHHH
SDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRLSIGIQSFNDAHLKALGRIHG
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
EREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNT
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCE
LFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLG
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCEEECCEEE
IGAGAHGKLSFPNRILRQMRHKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLT
ECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHC
DGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIKPTELGRRFLNDLQEMFLKD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFN
CCEEEECCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCCEEEEEECCHHHHHCCCCCCC
SHAAPGKPDSHEIPEDAYLDALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHHH
SDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRLSIGIQSFNDAHLKALGRIHG
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
EREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNT
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCE
LFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLG
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCEEECCEEE
IGAGAHGKLSFPNRILRQMRHKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLT
ECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHC
DGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIKPTELGRRFLNDLQEMFLKD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]